[PDF] Top 20 Selectivity Mechanism of the Voltage-gated Proton Channel, H V

... The voltage-gated proton channel, H V 1, has been implicated in numerous biological functions in humans 1 : charge compensation during the respiratory burst of phagocytes killing ... See full document

... of voltage gating by DpH is distinct from permeation. Pathognomonic of H V 1 is a strict correlation between the g H –V rela- tionship and V rev , in which V threshold ... See full document

... allosteric mechanism becomes indistinguishable from the HH ...single channel current amplitude in H V 1. Unitary H þ currents are 10 3 smaller than those of most ion channels and ... See full document

... of voltage- gated proton channels in 1972 in bioluminescent dinoflagellates, where they were thought to trigger the fl ash by activating ...luciferase. Proton channel genes were ... See full document

... preserved proton specificity, whereas Ser, Ala, or His substitution for Asp at this position re- sulted in anion permeability in both species, strongly suggesting that the selectivity mechanism is ... See full document

... exhibit proton currents, single mutations were intro- duced into H V 1 based on corresponding residues in the nonconducting ...the selectivity mechanism of the proton ...into ... See full document

... g H value for the increased pH i would lessen the apparent saturation of the curve, making a Boltzmann fit even less ...of H + current turn-on persist during depolarizations sustained for several minutes ... See full document

... a voltage-gated proton channel in humans [21], mice, and ...of proton channel [35], although some of its proponents have abandoned this idea ...are voltage-gated ... See full document

... age-gated proton channels has led to the conclusion that their fundamental function is acid extrusion from cells ...[14]. Proton channels are perfectly selective for ...[1,2,14]. ... See full document

... of voltage-gated proton channels (H V 1s) to the voltage-sensing domain (VSD) of other voltage-gated ion channels raises the central question of whether H ... See full document

... inhibited H + currents 80 times more potently than Ca 2+ currents, making it useful in separating these currents in Helix ...as proton currents were identi fi ed in several new cells and species each year, ... See full document

... g H was calculated from tail current amplitudes instead of I H ...g H –V relationship. For this purpose, we have ad- opted the voltage at which the g H is 10% of its maximal ... See full document

... TE. Voltage-Gated Proton Channels: Molecular Biology, Physiology, and Pathophysiology of the H V ...doi:10.1152/physrev.00011.2012.—Voltage-gated proton channels ... See full document

... Conclusion Voltage-gated proton channels are widely expressed, occur- ring in species ranging from unicellular dinoflagellates and coccolithophores to ...proton selectivity. The protein ... See full document

... including voltage-gated proton channels. H + channels in microglia have properties similar to those in other ...for H + channels has yet been demon- strated explicitly in microglia, it ... See full document

... a proton selective channel? With the basics of proton transfer in mind, let us take up the question how a proton channel may select for ...A proton channel requires two ... See full document

... the voltage-gated proton channel is a separate en- tity from the NADPH oxidase ...a proton channel should be ...a proton chan- ...in H currents. Furthermore, all ... See full document

... E. Voltage-Gated Proton Channels and Other Proton Transfer ...the proton pathway is formed mainly by water molecules present in the protein, but its function is regulated by titratable ... See full document

... about voltage-gated proton channels that remain ...the proton channel, most of the obvious and less obvious structure–function questions are still wide ...the proton ... See full document

... associated with a reciprocal decrease in H + occupancy at another interacting side chain. Although side chain pK a values in folded proteins can be measured using spectroscopic methods such as nuclear magnetic ... See full document