[PDF] Top 20 A Sensitive, Quantitative Assay for Human Immunodeficiency Virus Type 1 Integration

... one assay) are listed in Table 1. In this particular assay, as few as 5 proviruses in a 50-␮l sample were ...cells, 1 provirus in 10,000 cells could be reliably measured in this ...measure ... See full document

... Measuring human immunodeficiency virus type 1 (HIV-1) RNA in plasma has enabled the pathophysiology of the infec- tion to be studied, and this parameter, which directly reflects ... See full document

... The limit of detection for the ultrasensitive method was 50 viral RNA copies/ml of plasma. While the method was also able to detect samples with lower titers, a single measurement cannot accurately assess viral burden ... See full document

... CD4 1 cell number of 549. HIV-1- suppressive activity of CD8 1 T cells was comparable to that of mixtures of endogenously infected autologous CD4 1 T cells and CD4 1 cells separated in ... See full document

... TM contributes density to the bilayer region. The internal regions of the immature virion and the VLP were indistin- guishable; however, subtle differences were seen in the region of the membrane. The membrane ... See full document

... of virus pellets, were centrifuged at 23,500 3 g for 1 h in a refrigerated microcentrifuge (centrifuge ...the virus pellets either were processed immediately for the ES bDNA assay or were ... See full document

... receptor CXCR4, as well as with soluble ICAM-1, anti-LFA-1, anti-ICAM-1, and anti- ICAM-2 blocking antibodies, indicating the requirement for the LFA-1/ICAM interaction. Treatment of periph- ... See full document

... by human immunodeficiency virus type 1 (HIV-1) is usually assessed by counting multinucleated giant cells (syncytia) visualized by light ... See full document

... of 1% sodium dodecyl sulfate and 100 ␮g/ml proteinase K and extracted with phenol-chloroform ...DNA, 1 to 2 mg genomic DNA was electrophoresed on a ...the integration site, was isolated from the ... See full document

... in permissive feline cells renders them able to restrict HIV-1, and this antiviral effect is partially relieved by treatment with CSA (Fig. 3). An incomplete rescue of HIV-1 infectivity by CSA in feline ... See full document

... Yeast transformation and reverse transcription assay. BsrGI- and PvuII- linearized, gel-purified pH⌬RT1 (100 to 200 ng) and the PCR product contain- ing the HIV-1 RT domain (200 to 500 ng) were ... See full document

... Total cellular RNA purified with RNeasy silica columns was added to the Amplicor test to quantitate HIV-1 RNA from PBMCs and tonsil biopsy specimens. In contrast to the extrac- tion of plasma, the extraction ... See full document

... to quantitative PCR to assess proviral burden and by flow cytom- etry to assess reporter gene ...Table 1 and ...(Table 1 and ...reporter virus (Table 1 and ... See full document

... SI and NSI sequences can be distinguished by an HTA directed to the V3 coding region. HTA is able to distinguish between heteroduplexes based on the number of mismatches between the probe sequence and the test sequence ... See full document

... 2, quantitative and qualitative changes in HIV-1 envelope-specific serum antibody were evident during the first several months postinfection in both groups of SHIV- infected ...by 1 month ... See full document

... 3593 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Using purified integration protein IN from human immunodeficiency virus HIV type 1 and oligonucleotide mimics of vir[r] ... See full document

... in human immunodeficiency virus (HIV) infec- tion, since the extremely high mutation rate results in rapid swarm development and the dominance of certain quasispecies is strongly associated with ... See full document

... rapid-turnover assay was performed every third day ...mutant virus ligated to the 5⬘ long terminal repeat (LTR) of Lai and was named pRap1 (to designate the rapid ...mutant virus that conferred the ... See full document

... of virus as measured by EGFP fluorescence from these cells steadily declined ...some virus declined in a much more gradual manner ...ceasing virus expression, a continual and synchronous decline in ... See full document

... of human immunodeficiency virus type 1 (HIV-1) disease pro- gression (3, 4, 27); however, their effects appear to be of lim- ited duration (2, ...the virus remains ... See full document