[PDF] Top 20 Sensitive and Specific Method for Rapid Identification of Streptococcus pneumoniae Using Real Time Fluorescence PCR
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Sensitive and Specific Method for Rapid Identification of Streptococcus pneumoniae Using Real Time Fluorescence PCR
... our method can be used as a simple confirmatory assay due to increased sensitivity and specificity over standardized methods but is not practical for routine testing due to existing standard biological assays for ... See full document
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Rapid Identification of Penicillin and Macrolide Resistance Genes and Simultaneous Quantification of Streptococcus pneumoniae in Purulent Sputum Samples by Use of a Novel Real Time Multiplex PCR Assay
... by using Light- Cycler Software ...calculation method. After amplification, 10 l of the PCR product was separated by electrophoresis on a 3% agarose gel (Cambrex BioScience/Rockland, ...multiplex ... See full document
5
Quantitative Detection of Streptococcus pneumoniae in Nasopharyngeal Secretions by Real Time PCR
... S. pneumoniae (17). For the diagnosis of CAP due to S. pneumoniae, detection of antibodies to pneumolysin in serum (3, 7), detection of pneumolysin antigen in sputum or NPSs (3), and detection of the ... See full document
6
Development of a Real Time Fluorescence PCR Assay for Rapid Detection of the Diphtheria Toxin Gene
... the real-time PCR assay enabled de- tection of the tox gene in clinical specimens when standard PCR proved ...inadequate. Real-time PCR is sensitive, ... See full document
7
Rapid Identification of Methicillin Resistant Staphylococcus aureus Using Real Time PCR
... laboratories using conventional methods is time ...of real time PCR for the detection of MRSA. The PCR assay was evaluated in clinical isolates of MRSA (n = 45) and methicil- lin ... See full document
6
Rapid Identification of Methicillin ResistantStaphylococcus aureus and Simultaneous Species Confirmation Using Real Time Fluorescence PCR
... Staphylococcus aureus represents one of the most significant pathogens causing nosocomial and community-acquired infec- tions. Beta-lactam antibiotics are the preferred drugs for serious S. aureus infections (11). Since ... See full document
5
Rapid Diagnosis of Bacterial Meningitis by Real Time PCR and Fluorescence In Situ Hybridization
... the rapid diagnosis of meningitis that combines FISH and PCR based on the Gram ...the method of choice for samples in which multiple bacteria are detected by Gram stain- ...the identification ... See full document
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The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniaeand comparison with four other species specific PCR assays
... 5 PCR assays tested, which did not mis- identify ...S. pneumoniae. The approach using representative sequences rather than unfiltered data from Genbank enabled us to select the correct sites for ... See full document
8
Detection and Quantification of Infectious Hypodermal and Hematopoietic Necrosis Virus and White Spot Virus in Shrimp Using Real Time Quantitative PCR and SYBR Green Chemistry
... bioassay using indicator hosts, monitoring clinical signs, histopathology, dot blot and in situ hybridizations using a virus-specific gene probe, and PCR ...conventional PCR is the most ... See full document
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Evaluation of a Rapid Immunochromatographic ODK0501 Assay for Detecting Streptococcus pneumoniae Antigen in Sputum Samples from Patients with Lower Respiratory Tract Infection
... novel, rapid, and noninvasive test (ODK0501) to detect Streptococcus pneumoniae antigen was evaluated in a Japanese multicenter ...S. pneumoniae from sputum samples by an immunochromatographic ... See full document
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Rapid and Accurate Identification of Coagulase Negative Staphylococci by Real Time PCR
... phenotypic identification, but these are not always reliable and results are often operator dependent ...molecular identification of CNS has improved the accuracy of results and allows the ... See full document
5
Allele specific real time PCR testing for minor macrolide resistant Mycoplasma Pneumoniae
... SimpleProbe PCR coupled to melting curve analysis on respiratory ...other method in previous study, such as Chan et ...highly sensitive, accurate, time-saving and high throughput method ... See full document
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Application of pbp1A PCR in Identification of Penicillin Resistant Streptococcus pneumoniae
... by using PCR (6, 11, 20). The PCR is a rapid, specific, and sensitive method, and since it does not depend on the presence of viable organisms, it may be applicable in ... See full document
5
A highly sensitive, non invasive qPCR based strategy for direct quantification of Yersinia ruckeri in fish faeces
... Carson, J. & Wilson, T. (2003) Development of selective enrichment culture-polymerase chain reaction (SEC-PCR) for the detection of bacterial pathogens in covertly infected farmed salmonid fish, Deakin West, ... See full document
20
Evaluation of FRET real time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
... ensure rapid treatment administration and proper follow-up of the malaria attacks by the ...species identification in the same sample used for detec- tion of the parasite is also a greatest attribute of ... See full document
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Development of a simple and rapid sipA-directed PCR assay for serovar-independent specific identification of Salmonella enterica bacteria
... A total of 65 Salmonella human clinical isolates (representing 53 different serovars); in addition to 14 non-Salmonella isolates from 11 different bacterial genera were included in this study to check the specificity of ... See full document
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Detection and identification of aquatic birnaviruses by PCR assay
... the PCR assay with primer set PrB was effective in identifying all of the viruses tested in infected cell cultures (Table 1), this primer set was not effective in detecting virus in the clinical fish samples used ... See full document
5
Streptococcus pneumoniae Transmission Is Blocked by Type Specific Immunity in an Infant Mouse Model
... (ELISA) using whole bacteria (in which the plates were coated with the same ...anti-S. pneumoniae serum IgA titer in these ...Anti-S. pneumoniae titers did not impact pneumococcal colonization, as T4 ... See full document
12
Specific and Sensitive Detection of Neisseria gonorrhoeae in Clinical Specimens by Real Time PCR
... a real-time PCR assay for detection of ...highly specific and very ...generated PCR products that were not de- tected by the opa-1 ...these PCR products revealed the same newly ... See full document
7
Real-Time PCR with Serum Samples Is Indispensable for Early Diagnosis of Acute Q Fever
... it sensitive as a diagnostic ...burnetii real-time PCR positivity percentages in acute-phase and follow-up convalescent-phase serum samples from 65 patients with acute Q ...assay. PCR ... See full document
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