[PDF] Top 20 Sequencing Needs for Viral Diagnostics
Has 10000 "Sequencing Needs for Viral Diagnostics" found on our website. Below are the top 20 most common "Sequencing Needs for Viral Diagnostics".
Sequencing Needs for Viral Diagnostics
... Currently, we have the only fully automated DNA diagnostic signature prediction pipeline, described in detail elsewhere (2–4, 9). This pipeline selects regions of a target genome that are both conserved among strains (to ... See full document
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Universal Influenza B Virus Genomic Amplification Facilitates Sequencing, Diagnostics, and Reverse Genetics
... Generation of recombinant IBVs from genomic amplicons using recombination-based cloning strategy. The generation of recombinant IBVs is critical for basic research and is increasingly important in vaccine seed stock ... See full document
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Next-generation sequencing diagnostics of bacteremia in septic patients
... Raw reads were cleared from potential adapter contam- ination, quality controlled, and, if necessary, trimmed using BBDuk (https://sourceforge.net/projects/bbmap/). To pass the quality filter, read quality needed to ... See full document
13
Monitoring Viral Genetic Variation as a Tool To Improve Molecular Diagnostics for Mumps Virus
... real-time RT-PCR. A change in 3 to 4 cycles is equivalent to a 10-fold increase in sensitivity; therefore, samples at the lower limit of detection could be missed by the CDC* protocol (Fig. 3C and D; further assay ... See full document
9
Diagnostics of Neisseriaceae andMoraxellaceae by Ribosomal DNA Sequencing: Ribosomal Differentiation of Medical Microorganisms
... The logic incorporated in the recently published and now commercially available MicroSeq system (PE Biosystems, For- ster City, Calif.) is comparable to that in the RIDOM system (33). It also uses nonragged 16S rDNA ... See full document
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Sequencing viral genomes from a single isolated plaque
... Sequences were de novo assembled using the Newbler GS De Novo Assembler version 2.6 (Roche Diagnostics Corp., Indianapolis, IN) after the following pre-processing steps: 1) removal of host contamination by mapping ... See full document
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Proficiency Testing of Virus Diagnostics Based on Bioinformatics Analysis of Simulated In Silico High Throughput Sequencing Data Sets
... of viral reads from the HTS data set were shown to have decreasing success as sequences became more divergent from reference strains, as exemplified by MeV, with 82% identity on the nucleotide level to its closest ... See full document
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Viral diagnostics in plants using next generation sequencing : Computational analysis in practice
... The use of web based analytical environments such as Galaxy (Afgan et al., 2016) and Yabi (Hunter et al., 2012) appear to offer a solution, but their real advantage only comes if they are installed locally. Local ... See full document
10
System To Assess Genome Sequencing Needs for Viral Protein Diagnostics and Therapeutics
... SARS and, to a lesser extent, Ebola Zaire are outliers be- cause there was no near-neighbor sequence data in GenBank at the time of our analyses to narrow the list of protein or DNA signature candidates. Most of the ... See full document
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Standards for Sequencing Viral Genomes in the Era of High Throughput Sequencing
... (HT) sequencing technologies, great strides are being made toward the genomic characterization of this diversity ...of viral evolution, disease epi- demiology, surveillance, diagnosis, and countermeasure ... See full document
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Making the Leap from Research Laboratory to Clinic: Challenges and Opportunities for Next Generation Sequencing in Infectious Disease Diagnostics
... bacterial, viral, and fungal pathogens, a main attraction of NGS for clinical diagnosis is the ability to capture rare or un- suspected, yet potentially actionable, ... See full document
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Clinical evaluation of a dedicated next generation sequencing panel for routine glioma diagnostics
... NGS is primarily aiming at mutations and allows sim- ultaneous assessment of copy number alterations or of fusion genes, depending on the used technology. There is an increasing interest in the use of DNA genome wide ... See full document
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Replacing Traditional Diagnostics of Fecal Viral Pathogens by a Comprehensive Panel of Real Time PCRs
... DNA-based diagnostics are increasingly being used for diagnosis of viral ...conventional viral diagnostics consisting of viral culture and/or rotavirus and adenovirus rapid antigen ... See full document
6
Use of Amplification Refractory Mutation System PCR Assay as a Simple and Effective Tool To Detect HIV 1 Drug Resistance Mutations
... assay. Sequencing of HIV-1 reverse transcrip- tase was simultaneously performed for comparison, and discordant samples were tested with a Trugene HIV-1 genotyping ... See full document
10
Role of Extracellular Vesicles in Viral and Bacterial Infections: Pathogenesis, Diagnostics, and Therapeutics
... either viral or bacterial pathogens demonstrate several mechanisms to mediate the immune system, including effects to inhibit host EV effects to promote adaptive immune responses, indicating that better ... See full document
13
Viral Diversity by Deep Sequencing: Approaches to Analyzing Effects of Anti-HIV Treatments
... measured viral replication by assaying p24 in the mouse serum (Figure ...the viral titers between the vector-‐treated and control ...the viral load of patients treated with the clinical envAS vector ... See full document
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Molecular cloning and partial sequencing of hepatitis A viral cDNA.
... Molecular cloning of foot and mouth disease virus genome and Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest the cloned sequences were HAV in origin, not from other seq[r] ... See full document
9
Next-generation sequencing still needs our generation's clinicians
... Sanger sequencing may still be a cost- and time-effective genetic diagnostic testing approach in disorders with distinctive phenotypes associated with minimal genetic ...Whole-exome sequencing (WES) offers ... See full document
5
NGS barcode sequencing in taxonomy and diagnostics, an application in “Candida” pathogenic yeasts with a metagenomic perspective
... the sequencing depth obtainable with amplicon- based NGS could produce information on the real extent of the internal heterogeneity, possibly masked by the peculiarities of Sanger ... See full document
15
Comparison of the Biofire FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVPv1, and Luminex xTAG RVP Fast Multiplex Assays for Detection of Respiratory Viruses
... Respiratory virus testing. All NP swabs were tested with the four respiratory viral panels according to their respective package inserts. Ex- traction, when required, was performed using the bioMérieux EasyMag ... See full document
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