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[PDF] Top 20 Serine Phosphorylation of the Hepatitis C Virus NS5A Protein Controls the Establishment of Replication Complexes

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Serine Phosphorylation of the Hepatitis C Virus NS5A Protein Controls the Establishment of Replication Complexes

Serine Phosphorylation of the Hepatitis C Virus NS5A Protein Controls the Establishment of Replication Complexes

... phosphorylated protein comprised of three domains and tethered to membranes by an N-terminal amphi- pathic ...for NS5A to dimerize, while domains II and III are intrinsically disordered but have recently ... See full document

13

Serine phosphorylation of the hepatitis C virus NS5A protein controls the establishment of replication complexes

Serine phosphorylation of the hepatitis C virus NS5A protein controls the establishment of replication complexes

... phosphorylated protein comprised of three domains and tethered to membranes by an N-terminal amphi- pathic ...for NS5A to dimerize, while domains II and III are intrinsically disordered but have recently ... See full document

14

Phosphorylation of serine 225 in hepatitis C virus NS5A regulates protein-protein interactions.

Phosphorylation of serine 225 in hepatitis C virus NS5A regulates protein-protein interactions.

... IMPORTANCE Hepatitis C virus is an important human ...5A protein (NS5A) is the target for new antiviral drugs. NS5A has multiple func- tions during the virus life cycle, ... See full document

18

Phosphorylation of Serine 225 in Hepatitis C Virus NS5A Regulates Protein-Protein Interactions

Phosphorylation of Serine 225 in Hepatitis C Virus NS5A Regulates Protein-Protein Interactions

... IMPORTANCE Hepatitis C virus is an important human ...5A protein (NS5A) is the target for new antiviral drugs. NS5A has multiple func- tions during the virus life cycle, ... See full document

18

A Hepatitis C Virus NS5A Phosphorylation Site That Regulates RNA Replication

A Hepatitis C Virus NS5A Phosphorylation Site That Regulates RNA Replication

... infectious virus production and as this may be linked to phos- phorylation, we harvested supernatants of cells 48 h after the de- livery of our mutant RNAs and determined the titers of the amount of infectious ... See full document

6

Serine 235 Is the Primary NS5A Hyperphosphorylation Site Responsible for Hepatitis C Virus Replication

Serine 235 Is the Primary NS5A Hyperphosphorylation Site Responsible for Hepatitis C Virus Replication

... the hepatitis C virus nonstructural protein 5A (NS5A) undergoes transition between two phosphorylation states: hypo- and ...of serine residues is re- sponsible for ... See full document

14

Hepatitis B Virus DNA Replication Is Coordinated by Core Protein Serine Phosphorylation and HBx Expression

Hepatitis B Virus DNA Replication Is Coordinated by Core Protein Serine Phosphorylation and HBx Expression

... The hepatitis B virus (HBV) core protein forms the capsid of viral particles and is essential for viral genome DNA replication and ...The C terminus of core protein contains ... See full document

11

Y-Box Binding Protein 1 Stabilizes Hepatitis C Virus NS5A via Phosphorylation-Mediated Interaction with NS5A To Regulate Viral Propagation

Y-Box Binding Protein 1 Stabilizes Hepatitis C Virus NS5A via Phosphorylation-Mediated Interaction with NS5A To Regulate Viral Propagation

... ABSTRACT Replication of hepatitis C virus (HCV) is dependent on virus-encoded proteins and numerous cellular ...DDX3-interacting protein, Y-box binding protein 1 (YB-1), ... See full document

19

Phosphorylation of the Core Protein of Hepatitis B Virus by a 46-Kilodalton Serine Kinase

Phosphorylation of the Core Protein of Hepatitis B Virus by a 46-Kilodalton Serine Kinase

... Core protein is the major component of the core particle (nucleocapsid) of human hepatitis B ...the replication cycle of the virus, including RNA packaging, DNA synthesis, and recognition of ... See full document

8

High-Throughput Screening of the Yeast Kinome: Identification of Human Serine/Threonine Protein Kinases That Phosphorylate the Hepatitis C Virus NS5A Protein

High-Throughput Screening of the Yeast Kinome: Identification of Human Serine/Threonine Protein Kinases That Phosphorylate the Hepatitis C Virus NS5A Protein

... The hepatitis C virus NS5A protein plays a critical role in virus replication, conferring interferon resistance to the virus through perturbation of multiple ... See full document

12

Polo-Like Kinase 1 Is Involved in Hepatitis C Virus Replication by Hyperphosphorylating NS5A

Polo-Like Kinase 1 Is Involved in Hepatitis C Virus Replication by Hyperphosphorylating NS5A

... 2010 Hepatitis C virus (HCV) replication involves many viral and host ...a serine-threonine kinase, Polo-like kinase 1 (Plk1), as a potential host factor regulating HCV ...RNA ... See full document

11

Hepatitis C Virus NS5A Disrupts STAT1 Phosphorylation and Suppresses Type I Interferon Signaling

Hepatitis C Virus NS5A Disrupts STAT1 Phosphorylation and Suppresses Type I Interferon Signaling

... different hepatitis C virus (HCV) genotypes ...HCV NS5A proteins from different genotypes on IFN signal- ...GT3 NS5A proteins significantly inhibited IFN-induced IFN-stimulated response ... See full document

11

Multiple functions of capsid protein phosphorylation in duck hepatitis B virus replication.

Multiple functions of capsid protein phosphorylation in duck hepatitis B virus replication.

... The results showed that individual substitution of the various potential phosphor- ylation sites by alanine, including threonine 239, serine 257, and serine 259, failed to affect the lev[r] ... See full document

8

NS4B Self-Interaction through Conserved C-Terminal Elements Is Required for the Establishment of Functional Hepatitis C Virus Replication Complexes

NS4B Self-Interaction through Conserved C-Terminal Elements Is Required for the Establishment of Functional Hepatitis C Virus Replication Complexes

... NS3 protein with the 2E3 antibody, as described elsewhere (24). Virus titers (the 50% tissue culture infective dose [TCID 50 ]/ml) were calculated based on the method of Spearman and Ka ¨rber (23, ... See full document

14

Regulation of hepatitis C virus replication via threonine phosphorylation of the NS5A protein

Regulation of hepatitis C virus replication via threonine phosphorylation of the NS5A protein

... infectious virus, in vitro tran- scripts of either wild-type or mutant full-length mJFH-1 virus RNA were electroporated into Huh7 ...infectious virus, with the only significant reductions being ... See full document

11

Phosphorylation of hepatitis C virus-encoded nonstructural protein NS5A.

Phosphorylation of hepatitis C virus-encoded nonstructural protein NS5A.

... 56-kDa protein (p56) and a 58-kDa protein (p58), are produced from the hepatitis C virus (HCV) nonstructural region 5A ...at serine residues and that p58 is a hyperphosphorylated ... See full document

7

Phosphorylation of the hepatitis C virus NS5A protein in vitro and in vivo: properties of the NS5A-associated kinase.

Phosphorylation of the hepatitis C virus NS5A protein in vitro and in vivo: properties of the NS5A-associated kinase.

... For PPM, excised strips containing the phosphoprotein of interest were wetted briefly in methanol followed by water, incubated in 0.5% polyvinylpyrrolidone- 360–100 mM acetic acid for 30 min at 37°C to block ... See full document

11

Identification of a novel phosphorylation site in hepatitis C virus NS5A

Identification of a novel phosphorylation site in hepatitis C virus NS5A

... of NS5A is depicted, showing the location of the ...and NS5A was purified by using protein G beads and a polyclonal sheep anti- NS5A serum (Macdonald et ... See full document

6

Insights into the Complexity and Functionality of Hepatitis C Virus NS5A Phosphorylation

Insights into the Complexity and Functionality of Hepatitis C Virus NS5A Phosphorylation

... subsequent phosphorylation at S41, S37, and S33 by GSK-3 ...for NS5A, and intriguingly, S222 and S225 are predicted to constitute GSK3 phosphorylation ...mimicking phosphorylation, the S238D ... See full document

12

Insights into the complexity and functionality of hepatitis C virus NS5A phosphorylation

Insights into the complexity and functionality of hepatitis C virus NS5A phosphorylation

... subsequent phosphorylation at S41, S37, and S33 by GSK-3 ...for NS5A, and intriguingly, S222 and S225 are predicted to constitute GSK3 phosphorylation ...mimicking phosphorylation, the S238D ... See full document

13

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