[PDF] Top 20 Serotyping Streptococcus pneumoniae by Multiplex PCR
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Serotyping Streptococcus pneumoniae by Multiplex PCR
... by multiplex PCR. Capsular types identified by the PCR method invariably produced results concordant with the conventional serotyping ...the PCR data can guide the experimenter when ... See full document
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Identification of Less Common Streptococcus pneumoniae Serotypes by a Multiplex PCR Based Reverse Line Blot Hybridization Assay
... The exception was an isolate from China, which was identi- fied as serotype 33B/33D by mPCR/RLB and as serotype 33C by conventional serotyping. Both methods were repeated and the initial (discrepant) results ... See full document
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Development of an Automated and Multiplexed Serotyping Assay for Streptococcus pneumoniae
... the PCR amplicons, 20 l of the 1:100-diluted PCR mixture was transferred to a 96-well PCR plate and was heated for 10 min at ...the PCR was thawed and washed with 1⫻ tetramethylammonium ... See full document
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Sequential Multiplex PCR Approach for Determining Capsular Serotypes of Streptococcus pneumoniae Isolates
... conventional serotyping (three non- typeable and one type 9V rendered nontypeable on repeat capsular ...sequential multiplex PCR system were found to be highly specifically associated with no ...a ... See full document
8
Standardisation and evaluation of a quantitative multiplex real-time PCR assay for the rapid identification of Streptococcus pneumoniae
... +"Q!%'-!*$ E= $¶ " / "! <j #S! S. pneumoniae 8 +#S# ! - ! * $ E= $¶ " / [# "! >J X!### ![ #8 Z # S. pseudopneumoniaeX*"8 I!( W /[#"!j=!!"*}# M-E$¶"S. ... See full document
10
Evaluation of Semiautomated Multiplex PCR Assay for Determination of Streptococcus pneumoniae Serotypes and Serogroups
... the multiplex PCR assay results for four clinical isolates (isolates PN1010, PN1008, PN1001, and ...are multiplex PCRs: 3M (serotypes 3, 14, and 23F), 19M (serotypes 1 and 19F), GPM (serogroups 6, ... See full document
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Multiplex Urinary Antigen Detection for 13 Streptococcus pneumoniae Serotypes Improves Diagnosis of Pneumococcal Pneumonia in South African HIV Infected Adults
... In this study, the UAD assay was successfully adapted to be used in a cohort of HIV-infected South African adults with pneumonia and proved to be a diagnostic test of high accuracy. There was good correlation with ... See full document
11
From Quellung to Multiplex PCR, and Back When Needed, in Pneumococcal Serotyping
... against Streptococcus pneumoniae are based on selections of the over 90 different serotypes, which underlines the importance of serotyping for surveillance and vaccine efficacy ...a PCR-based ... See full document
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The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniaeand comparison with four other species specific PCR assays
... 5 PCR assays tested, which did not mis- identify ...pseudo- pneumoniae and a real-time, multiplex assay for rapid discrimination of the most important viridans strepto- cocci in bacterial cultures or ... See full document
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Multiplex PCR Based Reverse Line Blot Hybridization Assay To Identify 23 Streptococcus pneumoniae Polysaccharide Vaccine Serotypes
... S. pneumoniae-specific probes, was based on two for each of the serotypes represented in the 11-valent conjugate vaccine (serotypes 1, 3, 4, 5, 6B, 7F, 9V, 14, 18C, 19F, and 23F [shown in the top half of the ... See full document
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Simultaneous Detection of Streptococcus pneumoniae, S mitis, and S oralis by a Novel Multiplex PCR Assay Targeting the gyrB Gene
... S. pneumoniae strains, 20 ...other Streptococcus and 26 other coccus strains were obtained from the Culture Collection of the University of Gothenburg (CCUG; Gothenburg, Sweden), the Belgian Coordinated ... See full document
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Serotyping of Streptococcus pneumoniae isolated from Tehran by Multiplex PCR: Are serotypes of clinical and carrier isolates identical?
... for serotyping of ...of PCR-based serotyping systems ...as multiplex PCR are faster and simpler as well as have higher sensitivity and lower cost than the cultural ...Furthermore, ... See full document
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Sequetyping: Serotyping Streptococcus pneumoniae by a Single PCR Sequencing Strategy
... to Streptococcus pneumoniae, with no amplification observed for other species, including ...single PCR primer pair is cost-effective and sim- ... See full document
9
Use of multiplex PCR for simultaneous detection of four bacterial species in middle ear effusions
... the multiplex PCR for the study organisms was verified by performing the PCR with lysates from different ...S. pneumoniae were found in the GenBank. These are of Streptococcus mitis and ... See full document
5
Direct Streptococcus pneumoniae real-time PCR serotyping from pediatric parapneumonic effusions
... by PCR (assuming the serotype 7 detected was indeed 7 F and not 7A) were all among the six serotypes added to PCV13 that were not present in the original ...S. pneumoniae PCR-positive pleural fluid ... See full document
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Serotyping of Streptococcus pneumoniae Isolates from Nasopharyngeal Samples: Use of an Algorithm Combining Microbiologic, Serologic, and Sequential Multiplex PCR Techniques
... the correct sizes of the unknown bands. Fortunately, the se- quential MP-PCR algorithm is flexible and can be easily up- dated to reflect organizational changes or serotype fluctuations (8, 17, 20). As part of ... See full document
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Loop Mediated Isothermal Amplification Method Targeting the lytA Gene for Detection of Streptococcus pneumoniae
... S. pneumoniae type ...S. pneumoniae, a more specific and sufficiently reliable molecular diagnosis of ...S. pneumoniae in- fection should be ...S. pneumoniae strains and nine other ... See full document
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A Systematic Review and Meta analysis of the Diagnostic Accuracy of Nucleic Acid Amplification Tests for Tuberculous Meningitis
... Diagnostic method Conventional PCR Nested PCR Nested PCR Conventional PCR Conventional PCR Multiplex PCR Multiplex PCR Multiplex PCR Nested PCR Nested PCR Conventional PCR Conventional P[r] ... See full document
16
Reduction of Streptococcus pneumoniae Colonization and Dissemination by a Nonopsonic Capsular Polysaccharide Antibody
... ABSTRACT Streptococcus pneumoniae colonization of the nasopharynx (NP) is a prerequisite for invasive pneumococcal disease (IPD). The marked reduction in IPD that followed the routine use of pneumococcal ... See full document
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Rapidly Increasing Prevalence of Penicillin Resistant Streptococcus pneumoniae in Middle Tennessee: a 10 Year Clinical and Molecular Analysis
... primer PCR assay described in the present study was highly sensitive (99%) and specific (100%) for the simultaneous de- tection of ...S. pneumoniae and high-level penicillin ...S. pneumoniae in ... See full document
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