[PDF] Top 20 Simian virus 40 T-antigen DNA helicase is a hexamer which forms a binary complex during bidirectional unwinding from the viral origin of DNA replication.
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Simian virus 40 T-antigen DNA helicase is a hexamer which forms a binary complex during bidirectional unwinding from the viral origin of DNA replication.
... 66, 1992 ROLE OF T ANTIGEN AS A REPLICATIVE DNA HELICASE the viral origin of DNA replication, a bilobed T-antigen helicase complex was observed operating simultaneously at both forks of [r] ... See full document
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Role of Single-Stranded DNA Binding Activity of T Antigen in Simian Virus 40 DNA Replication
... of DNA is displaced from the channel is not clear, but it probably involves a conformation change of the T-anti- gen ...4 helicase domain may provide an answer. T7 gene 4 helicase ... See full document
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Mutational Analysis of Simian Virus 40 T-Antigen Primosome Activities in Viral DNA Replication
... 5031 T antigens are in fact sufficient to mediate priming by pol-prim on RPA-coated ssDNA but that 5030 and 5031 T antigens differ in their ability to functionally coordinate an RPA–pol-prim ...the ... See full document
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AP1 enhances polyomavirus DNA replication by promoting T-antigen-mediated unwinding of DNA.
... stimulate T-antigen-mediated DNA unwinding. T antigen helps assemble the viral replication complex, and a key step is the T-antigen-mediated ... See full document
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Simian Virus 40 Large T Antigen Can Specifically Unwind the Central Palindrome at the Origin of DNA Replication
... does T antigen specifically unwind the site II sequence? Modeling of the presumptive double hexamer structure over 31-bp DNA using published structure information (3, 20, 25, 28, 29, 35, 36) ... See full document
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Phosphorylation of Simian Virus 40 T Antigen on Thr 124 Selectively Promotes Double-Hexamer Formation on Subfragments of the Viral Core Origin
... SV40 replication in vivo. Upon syn- thesis, T-ag is rapidly transported as monomers from the cy- toplasm to the nucleus ...Since hexamer formation is inde- pendent of the phosphorylation ... See full document
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Two Regions of Simian Virus 40 T Antigen Determine Cooperativity of Double-Hexamer Assembly on the Viral Origin of DNA Replication and Promote Hexamer Interactions during Bidirectional Origin DNA Unwinding
... inhibited unwinding of closed circular duplex origin DNA and viral DNA replication (4–6, 14, ...the origin (the present study and reference ...in origin ... See full document
11
Replication of simian virus 40 origin-containing DNA during infection with a recombinant Autographa californica multiple nuclear polyhedrosis virus expressing large T antigen.
... polyhedrosis virus (AcMNPV) is a baculovirus that has been widely used for the expression of heterologous genes in insect cells (34, 36) and is being investigated for use as a potential viral insecticide ... See full document
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Sequence Requirements for the Assembly of Simian Virus 40 T Antigen and the T-Antigen Origin Binding Domain on the Viral Core Origin of Replication
... establish which sequences, in addition to site II, are re- quired for T-ag assembly events, we analyzed binding to larger subfragments of the core ...for hexamer formation and nucleotide ... See full document
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Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity.
... of DNA replication in eukaryotes have been productively studied by using simian virus 40 (SV40) (reviewed in references 17 and ...SV40 DNA replication requires a single ... See full document
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Analyses of the Interaction between the Origin Binding Domain from Simian Virus 40 T Antigen and Single-Stranded DNA Provide Insights into DNA Unwinding and Initiation of DNA Replication
... the T-ag OBD which are defective for DNA replication; the references to the individual mutations are ...double-stranded DNA substrates (i.e., the core origin [0], site I [I], ... See full document
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Topoisomerase I Associates Specifically with Simian Virus 40 Large-T-Antigen Double Hexamer–Origin Complexes
... this complex based on the optimum amounts of enzyme needed to nick origin DNA dur- ing a DNA-unwinding ...stoichiometry from our present data, we can esti- mate that there are ... See full document
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Role of the Hydrophilic Channels of Simian Virus 40 T-Antigen Helicase in DNA Replication
... 4 helicase, LTag undergoes an open-filament-to-closed-ring transition when assembly of the hexamer with DNA is ...4 helicase domain and ...LTag hexamer was visualized with a clear ... See full document
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The zinc finger region of simian virus 40 large T antigen is needed for hexamer assembly and origin melting.
... The failure of the zinc finger mutant T antigens to form hexamers in response to ATP may account for defects in subsequent steps in the initiation of DNA replication; we propose that hex[r] ... See full document
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Mutation of the cyclin-dependent kinase phosphorylation site in simian virus 40 (SV40) large T antigen specifically blocks SV40 origin DNA unwinding.
... A mutant T antigen carrying alanine in place of Thr-124 T124A also failed to replicate SV40 DNA in vitro and was defective in its ability to initiate replication in vitro Knowledge of eu[r] ... See full document
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Identification of simian virus 40 T-antigen residues important for specific and nonspecific binding to DNA and for helicase activity.
... 4858 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have previously identified three regions called elements in the DNA-binding domain of simian virus 40 large tumo[r] ... See full document
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The T-antigen-binding domain of the simian virus 40 core origin of replication.
... The lesser role of these intervening sequences is probably related to T-antigen binding rather than to cruciform formation because the central base pair at position 5243 would have no ef[r] ... See full document
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cdc2 phosphorylation of threonine 124 activates the origin-unwinding functions of simian virus 40 T antigen.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Binding of[r] ... See full document
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Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication.
... In the presence of RF-A, a eucaryotic single-stranded DNA-binding protein required for origin-specific unwinding and the initiation of DNA replication 23, 81, and a topoisomerase which r[r] ... See full document
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Monoclonal antibodies as probes for a function of large T antigen during the elongation process of simian virus 40 DNA replication.
... 411 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Various monoclonal antibodies specific for simian virus 40 large tumor antigen T antigen inhibit the elongation proc[r] ... See full document
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