[PDF] Top 20 Simian virus 40 mutants carrying two temperature-sensitive mutations.
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Simian virus 40 mutants carrying two temperature-sensitive mutations.
... Double mutants of A:B, A:C, and A:BC complementation groups, like their A parent, were defective in viral DNA replication, in the induction of host DNA synthesis, and in the transformati[r] ... See full document
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DNA infectivity and the induction of host DNA synthesis with temperature-sensitive mutants of simian virus 40.
... This, and the fact that mutants of the A group induce host DNA synthesis in CV-1 cells although they do not replicate viral DNA at 40 C 5, 18 suggested either that an early function othe[r] ... See full document
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Biological and biochemical studies of cells transformed by simian virus 40 temperature-sensitive gene A mutants and A mutant revertants.
... Virus recovered from both of the tsA30-transformed cell lines was temperature sensitive for viral DNA synthesis in BSC-1 cells, as is characteristic of SV40 A mutants Table 2.. That reco[r] ... See full document
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Temperature-sensitive BC mutants of simian virus 40: block in virion assembly and accumulation of capsid-chromatin complexes.
... In addition, we have tested the state of the VP1 protein produced in cells infected with selected mutants of the BC group tsBC216, tsBC223, or tsB248 and found that a stable form of this[r] ... See full document
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Temperature dependency for maintenance of transformation in mouse cells transformed by simian virus 40 tsA mutants.
... The great majority of mouse embryo fibroblast transformants generated with tsA mutant virus were temperature sensitive for transformation in all three assays, regardless of the input mul[r] ... See full document
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Temperature-sensitive mutants identify crucial structural regions of simian virus 40 large T antigen.
... SCHWEDES,' AND PETER TEGTMEYER1* Department of Microbiology, State University of New York, Stony Brook, New York 11794-8621,' and Department of Microbiology, Specialized Cancer Research [r] ... See full document
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Characterization of simian cells tranformed by temperature-sensitive mutants of simian virus 40.
... In addition, the role of the gene A product if any in the maintenance of the transformed 1106 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Seven lines derived from p[r] ... See full document
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Separation of lytic and transforming functions of the simian virus 40 A region: two mutants which are temperature sensitive for lytic functions have opposite effects on transformation.
... 518-528 0022-538X/81/050518-11$02.00/0 Separation of Lytic and Transforming Functions of the Simian Virus 40 A Region: Two Mutants Which Are Temperature Sensitive for Lytic Functions Hav[r] ... See full document
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Viral DNA Synthesis in Cells Infected by Temperature-Sensitive Mutants of Simian Virus 40
... For the initial screening of mutants data in Table 1 the 24 well plates of Linbro Chemical Co., New 116 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Temperature-sens[r] ... See full document
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Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.
... Purified T antigens T ag were either not exposed - or exposed + to a heat shock for 10 min at 39°C, incubated in replication buffer containing plasmids with the SV40 origin of replicatio[r] ... See full document
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Temperature-Sensitive Mutants of Simian Virus 40: Infection of Permissive Cells
... Plaque characteristics of wild-type and mutant simian virus 40 SV40 stocks propagated at 32 C Virus Efficiency of plating39 C/32 C Time of plaque appearance in days d Infected cultures w[r] ... See full document
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Products of complementation between temperature-sensitive mutants of simian virus 40.
... These results imply that i the D product may be synthesized late in infection; ii therefore, any defect exhibited by D virions early in infection probably results from the introduction o[r] ... See full document
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Temperature-sensitive mutants of simian virus 40 selected by transforming ability.
... Therefore, to analyze further the mechanism of transformation, we have tried to isolate mutants of SV40 i which are temperature sensitive in their ability to transform nonpermissive rat [r] ... See full document
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Temperature-sensitive mutants of Japanese encephalitis virus.
... Monolayers were infected with virus at a multiplicity of 1 PFU per cell in M199 containing 7.5% bovine serum and 0.2 jLg of Act D per ml and incubated at the appropriate temperature.. At[r] ... See full document
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Construction and analysis of viable deletion mutants of simian virus 40.
... The SV40 map location and size of the deletions were determined by the Si nuclease mapping procedure 18 and from the change in mobility of fragments produced by Hind II+III endonuclease [r] ... See full document
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Characterization of temperature-sensitive mutants of Pichinde virus.
... In cells infected with ts2, all proteins were detectable; however, the amounts of NP, GPC, and L proteins detectable were reduced in cells incubated at the nonpermissive temperature as c[r] ... See full document
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Nucleotide sequence analysis of two simian virus 40 mutants with deletions in the late region of the genome.
... Partial chemical degradation pattern of the DNA restriction fragment encompassing the deletion of each mutant: a fragment AtuI1392-HaeIII,218 from dl-1261 and b fragment AtuI1392-HaeIII1[r] ... See full document
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Creation and characterization of temperature-sensitive mutants of the lck tyrosine protein kinase.
... Mutations identical to those responsible for the temperature-sensitive phenotypes of the tsNY68 and tsNY72-4 v-src mutants rendered this activated kck gene temperature sensitive for both[r] ... See full document
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Studies of temperature sensitive mutants of polyoma virus
... This conclusion may, however, · be invalid when the multiplicity of infection is high as in this experiment; the time of the temperature sensitiv:e step may be overestimated if, within t[r] ... See full document
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Temperature-sensitive Mutants of Respiratory Syncytial Virus
... After ts mutants had been selected in BK tissue and stocks of virus prepared in these cells, further characterization was performed in a heteroploid cell line HeLa in which RS virus prod[r] ... See full document
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