[PDF] Top 20 Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein.

... 1-8 Copyright C 1977 American Society for Microbiology Solubilization ofthe Epstein-Barr Virus-Determined Nuclear Antigen and Its Characterization as a DNA-Binding Protein JANoS LUKA,* W[r] ... See full document

... to DNA) and EBNA-2 (which interacts indirectly with ...hepatocyte nuclear factor 1 (8, ...box- binding protein, TFIIB, and various TATA box-binding pro- tein-associated factors (10, 21, ... See full document

... to its well-defined role as a repressor of EBNA-2-mediated activation of transcription (via interac- tion with J␬), can also activate gene ...to its amino terminus and a glutamine-proline-rich domain in ... See full document

... Whole-cell DNA was extracted, and quantitative PCR with primers to either DS or ␤ -actin was used to determine the relative ratio of EBV genome to cellular ...top protein schematic represents Flag-tagged ... See full document

... The Epstein-Barr Virus (EBV) Nuclear Antigen 1 (EBNA1) protein is required for the establishment of EBV latent infection in proliferating ...multifunctional ... See full document

... large protein ( ...fusion protein sterically blocking the ...GAL4- binding sites positioned either very far upstream of the tran- scription start site at position 2 700 (pG5LTR-HIV; ... See full document

... and nuclear extracts from various cells lines, including at least four of the BJAB clones expressing EBNA 3A, 3B, or 3C, Raji, and EBV-transformed LCLs ...expected, nuclear extracts from EBV- infected BL ... See full document

... viral DNA inhibitor PAA on BFLF2 ...BFLF2 protein expression in different cell lines, either uninduced or following activation with TPA and sodium butyrate ...capsid antigen (BLRF2) was ... See full document

... Presence and relative amount of the 53K component in different cell types Relative amt of 53K' EBNA Origin Cell Undetectable Normal B-lymphocytes Undetectable Protein A-stimulated Blymph[r] ... See full document

... Epstein-Barr virus (EBV) is a DNA tumor virus that is associated with the pathogenesis of endemic Burkitt’s lym- phoma, Hodgkin’s lymphoma, and posttransplant ...EBV nuclear ... See full document

... FIG 7 EBNALP enhancer sites are important for their direct target gene expression. (A) (Left) LCL ChIP-seq tracks for EBNA2, EBNALP, EP300, EBF1, SPI1, BATF, SP1, and RNA Pol II ChIA-PET linkages at the EP300 locus are ... See full document

... DNA filter binding assays and DNase I footprinting experiments showed that the intact Raji EBNA-1 protein recognized the two binding site loci in oriP and the BamHI-Q locus and no other [r] ... See full document

... GAL4 DNA binding domain (amino acids 1 to ...GAL4 DNA binding domain and a hem- agglutinin (HA) epitope were constructed by inserting the EBNA1 fragment between the NdeI and BamHI sites (for ... See full document

... by Epstein-Barr virus (EBV) in vitro results in their immortalization into lymphoblastoid cell lines (LCLs); this latency program is controlled by the EBNA2 viral transcriptional activator, which ... See full document

... SMN protein and, conversely, of EBNA2wt and SMN⌬Tudor to determine whether the deletion of the contact domain for SMN on EBNA2 and vice versa resulted in a different subnuclear localization and/or association with ... See full document

... that its solubility under low-salt conditions was dramati- cally increased, a property that might reflect the ability of these proteins to act as chaperones for EBNA1 in ...Recovered DNA fragments were ... See full document

... In addition to the two induction systems used to study transcription of EBERs and EBNA mRNAs, we also looked at EBNA abundance in Raji cells which had been induced to make early antigens[r] ... See full document

... Viral capsid antigen production was stimulated with a phorbol ester, and the Epstein-Barr virus nuclear antigen cells were fixed in suspension to eliminate loss of antigen during the dry[r] ... See full document

... Thus, the findings that there is no inverted repeat within 10 min of lambda exonuclease digestion, the size 10 x 106 daltons of the terminus of the long of most EBV DNA molecules was bet[r] ... See full document

... Rta binding to the EBV genome during early replication, our results yielded important insights regarding late gene promoter regula- ...is determined primarily by sequences near the TATA box and appears to ... See full document