[PDF] Top 20 Specific effect of interferon on the herpes simplex virus type 1 transactivation event.

... In order to determine the in vivo effect of IFNs on HSV-1 replication, three different cell lines, HEp-2 human epithelial fibroblasts, Vero African green monkey kidney, and WISH human am[r] ... See full document

... inhibitory effect of Us11 on IFN production is PACT dependent or ...by interferon regulatory factor 3 (IRF3)-binding enhancer elements (IRF3-Luc), but not by ␬B el- ements ( ␬ B-Luc) ...Fig. 1, while ... See full document

... cellular interferon-stimulated genes (ISGs) after infection with herpes simplex virus type 1 (HSV-1) or human cytomegalovirus (HCMV) was ...either virus in the ... See full document

... Demand for insights into the protective immunologic mechanisms elicited by novel vaccines is at an all-time high. We have recently shown that an experimental prophy- lactic live-attenuated HSV-1 vaccine generates ... See full document

... of interferon genes (STING), and the HSV-1 gene product glycoprotein G-1 (gG1) in microglia, astrocytes, and whole brain samples were performed as described previously by our laboratory ... See full document

... The level of gene expression of late and early genes were determined by RT-PCR. For this purpose, vero cells were infected with HSV-1 in 24 well plates at 37°C for 1 hr, then cells were overlaid with medium ... See full document

... and effect of IFN- g on clearance of HSV from the nervous ...ble 1 and ...(CD4 1 and CD8 1 ) together with low levels of cell-associated IFN- g in ganglia but not in brain stems (Table ... See full document

... ABSTRACT Type I interferons (IFNs), as major components of the innate immune system, play a vital role in host resistance to a variety of ...by type I IFNs activates the Janus kinase-signal transducer and ... See full document

... IFN effect is on biological latency) ...dampening effect of IFN-␥ maintaining biological latency in most of the 129/Sv//Ev ...genital herpes disease in females, but not in males, is consistent with ... See full document

... The alpha protein ICP27 has been suggested to play an essential role in HSV-1 gene activation because viral mutants containing ts lesions in the ICP27 gene fail to accumulate gamma 2 and[r] ... See full document

... infectious virus in a plaque ...(Fig. 1). HSV-1 was reac- tivated from latency in 100% of these cultures by day 6 after culture initiation, as assessed by the presence of infectious virus in ... See full document

... detectable effect on expression of the gD gene in HSV-infected cells ...a transactivation domain primarily in the context of promoters with upstream ICP4 binding ... See full document

... including herpes simplex virus type 1 ...␣ 1, - ␣ 4, - ␣ 5, - ␣ 6, - ␣ 9, and - ␤ , against HSV-1 ...inhibitory effect in viral titers recovered from the ... See full document

... the effect of strain using an F test produced a result of F 5 3, P 5 ...neal virus shedding at the acute stage of infection has been reported in GKO mice (4), and we too have noted this in GKO and RGKO mice ... See full document

... the specific cell ...signaling event in all cell types, yet results in different apoptotic outcomes due to differences in the subsequent signaling ... See full document

... oral herpes lesion. TU1-1 was confirmed as HSV-1 by immunocytochemical staining of plaques with a monoclonal antibody that is specific for HSV-1 gG, whereas no staining was observed ... See full document

... (Fig. 1), and quantitated by phosphorimaging. Values from the virus dilutions were then compared with the protein standard curve to obtain the amount of protein (in nanograms) per ...each virus, and ... See full document

... cells. Herpes simplex virus type 1 (HSV-1) replication is somewhat reduced in tissue culture in the presence of IFN, presumably due to decreased viral ...wild-type ... See full document

... 2187 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest BALB/c mice infected on the scarified cornea with herpes simplex virus type 1 strain 35 [HSV-135] rarely developed [r] ... See full document

... Analysis of the potential coding sequences within the 2.9-kb Sall DNA fragment of HSV-2 revealed an open reading frame of 479 amino acids which began with the methionine encoded at posit[r] ... See full document