[PDF] Top 20 Site-Specific Integration of an Adeno-Associated Virus Vector Plasmid Mediated by Regulated Expression of Rep Based on Cre-loxP Recombination
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Site-Specific Integration of an Adeno-Associated Virus Vector Plasmid Mediated by Regulated Expression of Rep Based on Cre-loxP Recombination
... of site-specific integration was determined to range from 10 to 40%, which is somewhat lower than efficien- cies reported in earlier ...with Rep expression units contained provirus at ... See full document
8
Herpes Simplex Virus Type 1/Adeno-Associated Virus rep+ Hybrid Amplicon Vector Improves the Stability of Transgene Expression in Human Cells by Site-Specific Integration
... challenged vector development in the field of gene ther- ...transgene expression over time, particularly when nonintegra- tive vectors, such as those based on adenovirus or herpes simplex ... See full document
13
Adeno-Associated Virus Type 2 p5 Promoter: a Rep-Regulated DNA Switch Element Functioning in Transcription, Replication, and Site-Specific Integration
... replication. Rep expression was derived from one of two plasmids: pCMVGFP-pT7Rep78, a low-level Rep-expressing con- struct, or pCMVRep78, a high-level Rep expression con- struct; ... See full document
10
Efficient Site-Specific Integration of Large Transgenes by an Enhanced Herpes Simplex Virus/Adeno-Associated Virus Hybrid Amplicon Vector
... hybrid vector can stably transduce the full-length dystrophin cDNA to human cells efficiently through site-specific genomic ...a vector containing a GFP-fused full-length human dystrophin cDNA ... See full document
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Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: a molecular switch for control of gene expression.
... be regulated by Cre-catalyzed recombi- nation, we designed an expression cassette in which the Luc cDNA and the HCMV promoter were separated by a spacer region flanked by loxP sites that would ... See full document
7
Reduction of MBS85 gene expression after the targeted integration of a transgene into the AAVS1 site using adeno-associated virus integration machinery
... transgene plasmid and the AAVS1 sequences in two ...annealing site (figure 3A). Recently integration of AAV into AAVS1 has been reported to accompany partial duplication of the MBS85 gene and can ... See full document
7
Efficient Integration of Recombinant Adeno-Associated Virus DNA Vectors Requires a p5-rep Sequence in cis
... of adeno-associated virus (AAV) and adenovirus (Ad) gene therapy vectors to generate an Ad-AAV hybrid vector allowing efficient site-specific integration with Ad ...of ... See full document
11
Conditional Site-Specific Integration into Human Chromosome 19 by Using a Ligand-Dependent Chimeric Adeno-Associated Virus/Rep Protein
... of plasmid p5/LUC and 50 ng of the expression plasmids pCMV/Rep68, pCMV/Rep1–491/P, and ...ITR/Hook-Neo plasmid and 10 g of the expression plasmid pCMV/Rep68 (lane 6), pCMV/Rep1–491/P ... See full document
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Role for highly regulated rep gene expression in adeno-associated virus vector production.
... Recombinant adeno-associated virus (rAAV) has many fea- tures of interest in the field of gene therapy (6, 14, 34, ...The vector is based on a defective, nonpathogenic human parvovi- ... See full document
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Roles of Adeno-Associated Virus Rep Protein and Human Chromosome 19 in Site-Specific Recombination
... Though site-specific integration is dependent upon either of the two large Rep proteins, the AAV ITRs are the only cis elements required for integration (34, 44, ...of Rep ... See full document
14
A Helper-Dependent Capsid-Modified Adenovirus Vector Expressing Adeno-Associated Virus Rep78 Mediates Site-Specific Integration of a 27-Kilobase Transgene Cassette
... helper virus (with E1/E3 deleted) in HD-Ad ...helper virus replicates in infected cells, which results on one hand in the production of viral proteins and on the other hand in coreplication of HD-Ad ...that ... See full document
11
Adeno-Associated Virus Rep Represses the Human Integration Site Promoter by Two Pathways That Are Similar to Those Required for the Regulation of the Viral p5 Promoter
... cooperative event that requires the partici- pation of all structural domains. Our model proposes that the repression mechanism is a direct effect of Rep78/Rep68 either blocking the start of transcription and/or binding ... See full document
15
Mutational analysis of adeno-associated virus Rep protein-mediated inhibition of heterologous and homologous promoters.
... large Rep proteins toward these promoters, which may override an indirect inhibitory activity of the indi- vidual Rep ...binding site and the TATA box, and reduced binding of Rep68 was also ... See full document
12
Mutational analysis of the adeno-associated virus rep gene.
... Each of the mutant genes has been expressed in cell culture and assayed for the following functions: i nuclear localization, ii AAV DNA replication, iii trans activation of the AAV capsi[r] ... See full document
12
Identification of an Adeno-Associated Virus Rep Protein Binding Site in the Adenovirus E2a Promoter
... initiation site that was protected by Rep68 (48). Rep protein affinity for the other transcription promoters cited has not been ...start site for transcription is homologous to the A-stem of the AAV ... See full document
11
Adeno-Associated Virus Rep Protein-Mediated Inhibition of Transcription of the Adenovirus Major Late Promoter In Vitro
... binding site. All of the Rep proteins have DNA-stimulated ATPase activity and function as DNA helicases (14, 48, ...between Rep and the template ... See full document
11
Transgenic or Plant Expression Vector-Mediated Recombination of Plum Pox Virus
... pox virus (PPV) were constructed: three mutants with mutations of the assembly motifs RQ and DF in the coat protein gene (CP) and two CP chimeras with exchanges in the CP core region of Zucchini yellow mosaic ... See full document
8
Cre Levels Limit Packaging Signal Excision Efficiency in the Cre/loxP Helper-Dependent Adenoviral Vector System
... helper virus so that novel, rational strategies may be developed to further reduce, if not eliminate, helper virus ...helper virus must have somehow escaped Cre-mediated packaging ... See full document
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Simple Purification of Adeno Associated Virus DJ for Liver Specific Gene Expression
... ed by genome copy number were produced and purified from 150 150-mm dishes. The cell density on the day of transfection was the most important factor in obtaining high yields of virus. The highest yield was ... See full document
5
Kinetics and Frequency of Adeno-Associated Virus Site-Specific Integration into Human Chromosome 19 Monitored by Quantitative Real-Time PCR
... of site-specific integration of AAV-2 into AAVS1 on ...of site-specific inte- gration were performed with the real-time PCR LightCycler ... See full document
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