[PDF] Top 20 Specific Sindbis virus-coded function for minus-strand RNA synthesis.
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Specific Sindbis virus-coded function for minus-strand RNA synthesis.
... In cultures infected with tsll of complementation group B, the synthesis of viral minusstrand RNA ceased, whereas the synthesis of 42S and 26S plus-strand RNAs continued for at least 5 h[r] ... See full document
11
Minimal Template Requirements for Initiation of Minus-Strand Synthesis In Vitro by the RNA-Dependent RNA Polymerase of Turnip Yellow Mosaic Virus
... mosaic virus (TYMV) RNA and use of nonstructured C-rich RNA templates, we conclude that the main determinant in the tRNA-like structure of TYMV RNA for initiation of minus-strand ... See full document
8
Modification of Asn374 of nsP1 Suppresses a Sindbis Virus nsP4 Minus-Strand Polymerase Mutant
... amplifying specific regions of the viral genome by RT-PCR and digesting the resulting cDNAs with appropriate restriction ...cDNA, RNA transcripts were made and trans- fected into CEF cells, and the ... See full document
9
Cis-acting Elements Important for Potato Virus X Minus-strand RNA Synthesis In Vitro and In Vivo
... 1999), sindbis virus (Lemm et ...for RNA synthesis or template specificity (for review, see Lai, ...for minus-strand RNA synthesis, such as TCV (Wang & Simon, ... See full document
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The role of the pseudoknot at the 3' end of turnip yellow mosaic virus RNA in minus-strand synthesis by the viral RNA-dependent RNA polymerase.
... positive-stranded RNA genome of turnip yellow mosaic virus (TYMV) is folded into a “tRNA-like struc- ture” ...viral RNA such as adenylation by ATP:tRNA nucleotidyltrans- ferase (9), aminoacylation ... See full document
7
Role for Conserved Residues of Sindbis Virus Nonstructural Protein 2 Methyltransferase-Like Domain in Regulation of Minus-Strand Synthesis and Development of Cytopathic Infection
... Plus-strand RNA synthesis early in virus replication depends on the ability of a functional minus-strand replicase to utilize the incoming RNA (due to virus ... See full document
14
Intracellular Localization of Poliovirus Plus- and Minus-Strand RNA Visualized by Strand-Specific Fluorescent In Situ Hybridization
... the virus-induced vesicles. The onset of viral plus-strand RNA synthesis in peripheral, separated clusters of vesicles led us to investigate whether these early clusters originated from ... See full document
8
Alphavirus Minus-Strand RNA Synthesis: Identification of a Role for Arg183 of the nsP4 Polymerase
... of RNA and DNA polymerases indicate that they share common structural features (13, 15, 25) and a common catalytic mechanism ...a specific role(s) cannot be assigned yet to this re- ...to function ... See full document
9
Role of the 3′ tRNA-Like Structure in Tobacco Mosaic Virus Minus-Strand RNA Synthesis by the Viral RNA-Dependent RNA Polymerase In Vitro
... for minus-strand synthesis in vitro was the ACCA end (10, 39, ...(13). Specific elements of the TLS are required for efficient replication in vivo (21, ...confer specific promoter ... See full document
10
Requirements at the 3′ End of the Sindbis Virus Genome for Efficient Synthesis of Minus-Strand RNA
... for minus-strand ...genomic RNA has previously been proposed as the initiation site for minus-strand RNA synthesis, giving rise to minus-strand RNA ... See full document
10
Initiation of minus-strand RNA synthesis by the brome mosaicvirus RNA-dependent RNA polymerase: use of oligoribonucleotide primers.
... mosaic virus (BMV) is a minor pathogen of cereal crops which is studied as a model system for RNA replication ...RNAs: RNA 1 (3.2 kb), RNA 2 (2.8 kb), and RNA 3 ...the RNA ... See full document
5
Secondary Structure of the 3′ Terminus of Hepatitis C Virus Minus-Strand RNA
... minimal RNA frag- ment required for efficient replication of the (⫺) strand spans nt ⫺239 to ⫺1 (43), which corresponds to the domain I iden- tified in the present ...of RNA synthesis (24). In ... See full document
11
A Pseudoknot in a Preactive Form of a Viral RNA Is Part of a Structural Switch Activating Minus-Strand Synthesis
... of minus-strand synthesis in newly infected ...mosaic virus, coat protein binding to specific 3 ⬘ -end sequences may compact the genomic RNA into its preactive structure, which ... See full document
11
Fate of Minus-Strand Templates and Replication Complexes Produced by a P23-Cleavage-Defective Mutant of Sindbis Virus
... synthesized minus-strand templates and both species of plus strands, and they contained the ex- pected RIa and RIb template structures that yielded normal- size RNase-resistant RF ...because ... See full document
12
In Vitro Synthesis of Minus-Strand RNA by an Isolated Cereal Yellow Dwarf Virus RNA-Dependent RNA Polymerase Requires VPg and a Stem-Loop Structure at the 3′ End of the Virus RNA
... dwarf virus (CYDV) RNA has a 5 ⴕ -terminal genome-linked protein ...template-dependent RNA-dependent RNA polymerase (RdRp) has been produced from a CYDV membrane-bound RNA polymerase by ... See full document
9
Capsid Protein Synthesis from Replicating RNA Directs Specific Packaging of the Genome of a Multipartite, Positive-Strand RNA Virus
... house virus (FHV), a member of the Nodaviridae fam- ily, is a nonenveloped, icosahedral insect virus whose genome consists of two positive-strand RNA molecules (3, ...the RNA- dependent ... See full document
10
Specific cessation of minus-strand RNA accumulation at an early stage of tobacco mosaic virus infection.
... To understand the replication mechanism of TMV RNA and also the function of the 130K and 180K proteins in replication, we examined the time course of accumulation of viral plus- and minu[r] ... See full document
8
Specific detection of hepatitis C virus minus strand RNA in hematopoietic cells
... a specific signal even when the primer had been omitted from the cDNA synthesis reaction in both serum (lane 5) and liver samples Figure ...(2 strand) or pos- itive (1 strand) strand ... See full document
8
Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis.
... 1874-1885 0022-538X/94/$04.00+0 Copyright © 1994, American Society for Microbiology Regulation of Sindbis Virus RNA Replication: Uncleaved P123 and nsP4 Function in Minus-Strand RNA Synt[r] ... See full document
12
Genetic analysis of the nsP3 region of Sindbis virus: evidence for roles in minus-strand and subgenomic RNA synthesis.
... These data showed that increased replication of the CR3.36 and CR3.39 genomes occurred during complementation and indicate that the ts defect is most likely a defect in nsP3 or nsP3-cont[r] ... See full document
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