[PDF] Top 20 Structural Analysis of the Hepatitis C Virus RNA Polymerase in Complex with Ribonucleotides
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Structural Analysis of the Hepatitis C Virus RNA Polymerase in Complex with Ribonucleotides
... The complex has been rotated 90° around a vertical axis with respect to the view shown in panel a, so that Arg32 (at the base of helix A) now lies in the ...rUTP complex, overall ...the C and P ... See full document
11
Hydrophobic and Charged Residues in the C-Terminal Arm of Hepatitis C Virus RNA-Dependent RNA Polymerase Regulate Initiation and Elongation
... W550A/UTP/Mn complex (light gray carbons, W550N mutant; dark gray carbons, UTP; green, Mn 2⫹ ...In complex “UTPa,” the phosphates are in close contact with both metal ions, resembling the classical binding ... See full document
12
Regulation of De Novo-Initiated RNA Synthesis in Hepatitis C Virus RNA-Dependent RNA Polymerase by Intermolecular Interactions
... The C terminus of NS5B does not affect intermolecular ...a C-terminal histidine tag to aid in ...DSF analysis of ⌬ 21 and PV mutant as for ... See full document
13
Quantitative Analysis of the Hepatitis C Virus Replication Complex
... replication complex is very similar to a model suggested by Miyanari et ...replication complex is composed of multiple copies of the viral nonstructural ...viral RNA, together with a few copies of ... See full document
12
Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3′ End of the Viral RNA
... viral RNA on the NS5B RdRp activity was not ...minus-strand RNA synthesis depends on an initial recognition and specific binding of the NS5B RNA polymerase or replicative complex to the ... See full document
6
Interaction with a Ubiquitin-Like Protein Enhances the Ubiquitination and Degradation of Hepatitis C Virus RNA-Dependent RNA Polymerase
... Hepatitis C virus (HCV), a member of the Flaviviridae fam- ily, is the major causative agent of non-A, non-B hepatitis ...single-stranded RNA genome en- codes a single polyprotein of ... See full document
11
Selection of RNA Aptamers That Are Specific and High-Affinity Ligands of the Hepatitis C Virus RNA-Dependent RNA Polymerase
... 1B). Analysis of the secondary structure of each sequence by the MFOLD program (21) revealed that the overall structure of the individual aptamers belonging to each class was conserved, with the common sequence ... See full document
9
Characterization of Soluble Hepatitis C Virus RNA-Dependent RNA Polymerase Expressed in Escherichia coli
... primer complex and a ribonucleotide are ...binary complex which then takes up a nucleotide to form the catalytically competent ternary complex (5, ... See full document
6
The Hepatitis C Virus RNA-Dependent RNA Polymerase Membrane Insertion Sequence Is a Transmembrane Segment
... The hepatitis C virus (HCV) RNA-dependent RNA poly- merase (RdRp), represented by nonstructural protein 5B (NS5B), is believed to form a membrane-associated replication complex ... See full document
6
Selection of 3′-Template Bases and Initiating Nucleotides by Hepatitis C Virus NS5B RNA-Dependent RNA Polymerase
... other RNA polymerases, is highly selective for ribonucleotides (NTP) over 2⬘-deoxyribonucleotides (2⬘-de- ...novo RNA synthesis by bovine viral diarrhea virus RdRp (6) and HCV NS5B ...T7 ... See full document
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Conformational Flexibility of the Dengue Virus RNA-Dependent RNA Polymerase Revealed by a Complex with an Inhibitor
... (SPR) analysis to demon- strate the binding of NITD107 to DENV ...SDS-PAGE analysis of purified recombinant DENV-3 ...single-stranded RNA (ssRNA) template entry, rNTP entry (dashed arrow at the back ... See full document
5
Identification of the Hepatitis C Virus RNA Replication Complex in Huh-7 Cells Harboring Subgenomic Replicons
... multiprotein complex. To determine the distribution of viral plus-strand RNA, fluorescence in situ hybridization (FISH) was ...T7 RNA polymerase (Roche Molecular Biochemicals, Mann- heim, ... See full document
6
Structural Determinant of Human La Protein Critical for Internal Initiation of Translation of Hepatitis C Virus RNA
... of hepatitis C virus ...the RNA recognition motif (RRM) encom- passing residues 112 to 184 of La protein [La (112–184)] interacts with the HCV IRES near the initiator AUG ...retains RNA ... See full document
12
The C-Terminal Hydrophobic Domain of Hepatitis C Virus RNA Polymerase NS5B Can Be Replaced with a Heterologous Domain of Poliovirus Protein 3A
... NS5B C-terminal transmembrane domain. The C-terminal hy- drophobic domain of HCV NS5B, encompassing amino acids 570 to 591 ...cis-acting RNA replication element identified previously by You et ... See full document
12
Comparative Analysis of Hepatitis B Virus Polymerase Sequences Required for Viral RNA Binding, RNA Packaging, and Protein Priming
... vitro RNA binding assay with bacterially ex- pressed and chaperone-reconstituted HP ...the C-terminal portion of RT, and the entire RNase H domain are dispensable for Hε binding ...the C terminus of ... See full document
9
Template Requirements for De Novo RNA Synthesis by Hepatitis C Virus Nonstructural Protein 5B Polymerase on the Viral X RNA
... the RNA polymerization reaction, but the quantities were not ...X RNA regulates the initiation specificity of RNA synthesis by ...novo RNA synthesis was selectively initiated, we prepared the ... See full document
13
Mechanism of De Novo Initiation by the Hepatitis C Virus RNA-Dependent RNA Polymerase: Role of Divalent Metals
... initiate RNA synthesis by a number of ...influenza virus, can use a combination of the two strategies, depending on the RNA to be synthesized ...Flaviviridae RNA-dependent RNA ... See full document
13
De Novo Initiation Pocket Mutations Have Multiple Effects on Hepatitis C Virus RNA-Dependent RNA Polymerase Activities
... of RNA synthesis with GTP in the absence of Mn 2⫹ , were capable of efficient RNA synthesis with GU (Table ...robust RNA synthesis with GUAUA, a GTPi analog that could form more stable base pairing ... See full document
11
Modulation of hepatitis C virus RNA Abundance.pdf
... HCV RNA, which occurs by an internal ribosome entry mechanism ...wild-type RNA. However, we were unable to detect full- length viral RNA by Northern analysis at this time ...viral RNA ... See full document
6
De Novo Initiation of RNA Synthesis by Hepatitis C Virus Nonstructural Protein 5B Polymerase
... of RNA synthesis ...tive-strand RNA synthesis and GTP for positive-strand synthe- sis in ...strand RNA viruses in that positive-strand RNA is more abun- dant than negative-strand RNA in ... See full document
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