[PDF] Top 20 Structural analysis of human immunodeficiency virus type 1 Gag protein interactions, using cysteine-specific reagents.

... NaCl, 1 mM EDTA, ...NaCl, 1 mM EDTA, 0.1% sodium dodecyl sulfate [SDS], 0.5% sodium deoxycholate, 1% Triton X-100, ...in 1 ml of PBS for each 10-cm-diameter ...in 1 ml of IPB plus ... See full document

... the Gag structural protein. For human immunodeficiency virus type 1 (HIV-1), this association is mediated in part by a myristate fatty acid ...ionic ... See full document

... the cysteine-specific cross-linkers BMH and bis-maleimidoethane or with the cysteine oxidation reagent ...In using any cross-linking agent, a natural concern is that putative assembly products ... See full document

... of Gag-processing intermediates, notably p41 and p38, which are not normally found in WT ...of Gag and Pol precursor polyproteins in oncogenic type C retroviruses, which do not carry a vif-like gene, ... See full document

... Nef protein containing the SDYQRL sequence was studied because it bound well to ␮ 1 in the yeast two-hybrid assay yet was devoid of biologic ...fusion protein, Nef-SDYQRL bound inef- ficiently to ... See full document

... Immunoblot analysis. Immunoblot analysis of viral proteins was performed as previously described ...Briefly, virus produced by transient transfection of 293T cells was pelleted by ultracentrifugation ... See full document

... WT Gag. The immunofluorescence analysis pre- sented above suggested that the LL189,190AA mutant may be defective for Gag ...mutant Gag to be rescued upon coexpression with WT ...assay, ... See full document

... of human ARF1, a critical host factor involved in HIV assembly and ...the specific interactions and consequences of such co-infections in different compartments of the body are not well ... See full document

... MA protein is targeted to the nucleus, and some evidence suggests that it contributes to the transport of the viral preintegration complex from the cytoplasm to the nucleus of nondividing cells (4, ...the ... See full document

... the protein subunit is required prior to continued assembly, such as is seen in the polymerization of bacterial flagellin ...on protein concentration suggests that several mole- cules of CA, rather than ... See full document

... Our analysis of viral DNA elongation shows that for the H12A, H16A, F185A, and D22 IN mutant viruses, the minus-strand strong-stop DNA product was produced in amounts similar to those of the intermediate and late ... See full document

... The protein sequences used to replace NC were UPRT and HXGPRT enzymes from ...chimeric Gag proteins which have the HIV-1 NC regions replaced by UPRT and HXGPRT were expressed well in transfected Cos7 ... See full document

... The individual proximal cysteine-histidine box mutations were exchanged for the wild-type sequences in the Pr55gag expression construct to determine whether binding to HIV-1 RNA in vitro[r] ... See full document

... during virus matu- ration, when the dimeric genomic RNA undergoes a stabiliza- tion event (19, 22, 23, ...the protein at sites where its chaperone activity is required; conversely, the chaperone ac- tivity ... See full document

... HeLa CD4 cells were transfected with pHXB2D, the cysteine substitution mutants, or a noninfectious mutant defective in the integrase gene, which HIV-1 ENVELOPE CYSTEINE MUTANTS... TSCHAC[r] ... See full document

... Recently, a gel mobility shift assay has been used to measure in vitro binding of purified bovine leukemia virus BLV gag proteins to BLV genomic RNA segments; in this report, NC was show[r] ... See full document

... Mutant infectivity and replication. The ability of the HIV-1 mutants examined above to carry out a single round of infec- tion was tested by using JC53bl-13 cells in an LTR-lacZ Tat complementation assay ... See full document

... The two regulatory protein coding regions for tat and rev that are essential for virus replication are intact, and transient expression of HIVgpt in COS7 cells results in assembly and re[r] ... See full document

... FACS analysis using a monoclonal antibody directed against the AU1 antigenic ...support virus binding could be directly compared to that of wt ...facilitate protein folding and subse- quent ... See full document

... Because of its structural similarity to the membrane protein M2 encoded by influenza virus and the previously reported 4- to 10-fold reduction in progeny virion production associated wit[r] ... See full document