[PDF] Top 20 Structure-Based Mutational Analysis of the NS3 Helicase from Dengue Virus
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Structure-Based Mutational Analysis of the NS3 Helicase from Dengue Virus
... The dengue virus NS3 protein contains protease, RNA helicase, 5 ⬘ -nucleoside triphosphatase (NTPase), and RNA 5 ⬘ - triphosphatase (RTPase) activities (1, 2, 5, 15, 20, ...21). NS3 ... See full document
5
Structure-Based Mutational Analysis of the Hepatitis C Virus NS3 Helicase
... RNA helicase activity and the ...wild-type helicase) still exhibited sufficiently high RNA helicase activity (for example, H293A and ...previous mutational studies (21, ...The structure ... See full document
9
Mapping the Interactions between the NS4B and NS3 Proteins of Dengue Virus
... the structure, function, and inter- actions of NS4B is required to support further antiviral develop- ...NS4B structure was proposed with two N-terminal amphipathic helices, three transmembrane heli- ces, ... See full document
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Structure of the Dengue Virus Helicase/Nucleoside Triphosphatase Catalytic Domain at a Resolution of 2.4 Å
... The NS3 protein from Dengue virus is a multifunc- tional protein of 69 kDa, endowed with protease, helicase, and nucleoside 5 ⴕ -triphosphatase (NTPase) activ- ...Thus, NS3 plays ... See full document
11
Identification of Inhibitor against Zika Virus NS3 Helicase
... Zika virus belongs to falvivirus family, which is an ...Zika virus have had instances in past years, but it has recently been declared as a health emergency by World Health ...Zika virus share the ... See full document
9
X-Ray Structure of the Pestivirus NS3 Helicase and Its Conformation in Solution
... and NS3 have enzymatic activities. NS3 has a molecular mass of about 76 kDa and features two main functional domains, each with different enzymatic ...of NS3, is a helicase belonging to super- ... See full document
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Mutagenesis of the Dengue Virus Type 2 NS3 Protein within and outside Helicase Motifs: Effects on Enzyme Activity and Virus Replication
... The helicase of the flavivirus Dengue virus type 2 (DEN-2) is a member of su- perfamily 2, which includes the helicases of the pestivirus Bo- vine viral diarrhea virus (BVDV) and the ... See full document
11
Crystal Structure of the NS3 Protease-Helicase from Dengue Virus
... (NS3 helicase domain [NS3hel]) (44– 46), the NS5 methyltransferase (10), and the RNA-dependent RNA polymerase catalytic domain (27, 49, ...complete NS3 and NS5 polypeptide chains are still lacking. ... See full document
11
Uncoupling of Protease trans-Cleavage and Helicase Activities in Pestivirus NS3
... full-length NS3 crystal ...the structure; residue numbers are indicated where ...reported structure exhibit relatively “open” statuses with limited intramolecular protease-helicase ... See full document
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Structurally Conserved Amino Acid W501 Is Required for RNA Helicase Activity but Is Not Essential for DNA Helicase Activity of Hepatitis C Virus NS3 Protein
... detailed structure of wild-type and mutant HCV helicase complexed with an ssRNA or ssDNA substrate by using molecular modeling ...this analysis, we iden- tified a putative -facial interaction ... See full document
12
Structure-guided mutagenesis of active site residues in the dengue virus two-component protease NS2B-NS3
... the NS3 protease and the peptide substrate were largely consistent with data obtained from substrate pro- filing studies ...Nile Virus (WNV) and dengue virus in complex with a partial ... See full document
8
Dengue Virus NS2B/NS3 Protease Inhibitors Exploiting the Prime Side
... detailed analysis of the specificity for dengue virus protease for P= side substrates to design the scaffold for specific ...pocket from the S3 to S4= position ...loop structure and ... See full document
10
Human Norovirus NS3 Has RNA Helicase and Chaperoning Activities
... vRNAs from vRNA ...RNA structure required for one vRNA function, like translation or encapsidation, may need disruption and refolding to facilitate another vRNA function, like vRNA replication, and vice ... See full document
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Autocatalytic Cleavage within Classical Swine Fever Virus NS3 Leads to a Functional Separation of Protease and Helicase
... of NS3 is ...HCV NS3-4A constructs (11), a single-chain CSFV NS4A3 protease was designed ...BVDV NS3 pro- tease, a minimal essential NS4A peptide has been determined for amino acids 2293 to 2329 ... See full document
12
In vitro processing of dengue virus type 2 nonstructural proteins NS2A, NS2B, and NS3.
... In order to confirm that the cleavage events observed in vitro occur at the same sites as those utilized in vivo and to confirm the identities of the proteins produced in vitro, we compa[r] ... See full document
11
Mutational analysis of the octapeptide sequence motif at the NS1-NS2A cleavage junction of dengue type 4 virus.
... To test this cleavage model in vivo, mutant constructs of DEN 4 virus NS1-NS2A cDNA were engineered to encode a single amino acid substitution at one of these eight positions or at the P[r] ... See full document
7
The Serine Protease and RNA-Stimulated Nucleoside Triphosphatase and RNA Helicase Functional Domains of Dengue Virus Type 2 NS3 Converge within a Region of 20 Amino Acids
... of dengue virus type 2 has a serine protease domain within the N-terminal 180 ...for NS3 to form an active protease involved in processing of the viral polyprotein ...RNA helicase activities ... See full document
9
NS3 from Hepatitis C Virus Strain JFH-1 Is an Unusually Robust Helicase That Is Primed To Bind and Unwind Viral RNA
... the NS3 helicase from the HCV JFH-1 strain contains sequence changes that enable it to bind rapidly and productively to a diversity of RNA unwinding targets, thereby transforming a relatively poor ... See full document
16
The NS4A Protein of Hepatitis C Virus Promotes RNA-Coupled ATP Hydrolysis by the NS3 Helicase
... in NS3 helicase assay buffer before being loaded onto a membrane sandwich, which consisted of a nitrocel- lulose membrane top layer ...ice-cold NS3 helicase assay ... See full document
8
The Hepatitis C Virus NS3 Protein: A Model RNA Helicase and Potential Drug Target
... HCV helicase is that, unlike other helicases, the protein binds RNA and DNA in a sequence specific ...HCV helicase has a distinctive nucleic acid stimulation profile (Suzich et ...HCV helicase ... See full document
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