[PDF] Top 20 RNA Structure Modulates Splicing Efficiency at the Human Immunodeficiency Virus Type 1 Major Splice Donor
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RNA Structure Modulates Splicing Efficiency at the Human Immunodeficiency Virus Type 1 Major Splice Donor
... The splicing event was con- firmed by cDNA sequencing, indicating that the HIV-1 5 ⬘ SD is joined to a cryptic 3 ⬘ SA at position 570 in the luciferase mRNA ...that splicing occurs on the luciferase ... See full document
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SRp40 and SRp55 Promote the Translation of Unspliced Human Immunodeficiency Virus Type 1 RNA
... the human SR proteins that we tested increased intracellular Gag abundance and su- pernatant VLP ...(CMV) major immediate-early promoter start site was inserted into the pcDNA ...The splice ... See full document
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Control of Human Immunodeficiency Virus Type 1 RNA Metabolism: Role of Splice Sites and Intron Sequences in Unspliced Viral RNA Subcellular Distribution
... exon splicing regulatory elements does not alter nuclear retention of HIV-1 unspliced ...suboptimal splicing (9) is responsible for the nuclear retention of HIV-1 unspliced env mRNA led us to ... See full document
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cis-acting sequences involved in human immunodeficiency virus type 1 RNA packaging.
... Retroviral RNA packaging is a specific process involving interactions among cis-acting sequences in the RNA genome and viral structural ...In human immunodeficiency virus type ... See full document
5
Repair of a Rev-Minus Human Immunodeficiency Virus Type 1 Mutant by Activation of a Cryptic Splice Site
... sarcoma virus (env and src) are both suboptimal because of nonconsensus polypyrimidine tracts or branch point sequences (15, ...of splicing (3, ...leader RNA, containing the major 5⬘ ss, and ... See full document
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RNA Structure and Packaging Signals in the 5′ Leader Region of the Human Immunodeficiency Virus Type 1 Genome
... FIG. 1. Mutant HIV-1 RNAs characterized in this ...HIV-1 RNA from strain HXB2, with the gag initiation codon shown with open lettering and the major 5⬘ splice donor (SD) ... See full document
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Acetylated Tat Regulates Human Immunodeficiency Virus Type 1 Splicing through Its Interaction with the Splicing Regulator p32
... HCMV major IE promoter, and its polyadenylation is directed by the simian virus 40 early PA signal ...(87). Splicing of LTR-Target and Target 3 can be studied by RT-PCR using primers 182 and 183 ... See full document
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A Hairpin Structure in the R Region of the Human Immunodeficiency Virus Type 1 RNA Genome Is Instrumental in Polyadenylation Site Selection
... viral RNA production, total cellular RNA was isolated from transfected C33A cells and analyzed by RNAse protection ...HIV-1 RNA was hybridized to a riboprobe complementary to the U3, R, and U5 ... See full document
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RNA Splicing at Human Immunodeficiency Virus Type 1 3′ Splice Site A2 Is Regulated by Binding of hnRNP A/B Proteins to an Exonic Splicing Silencer Element
... of human immunodeficiency virus type 1 (HIV-1) mRNAs is a complex process by which more than 30 different mRNA species are produced by alternative splicing of a single ... See full document
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Identification of a Domain in Human Immunodeficiency Virus Type 1 Rev That Is Required for Functional Activity and Modulates Association with Subnuclear Compartments Containing Splicing Factor SC35
... of human immunodeficiency virus Rev as a regulator of viral mRNA expression is tightly linked to its ability to shuttle between the nucleus and cytoplasm; these properties are conferred by a ... See full document
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Regulation of vif mRNA Splicing by Human Immunodeficiency Virus Type 1 Requires 5′ Splice Site D2 and an Exonic Splicing Enhancer To Counteract Cellular Restriction Factor APOBEC3G
... The human immunodeficiency virus type 1 (HIV-1) accessory protein Vif is encoded by an incompletely spliced mRNA resulting from splicing of the major splice ... See full document
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The human immunodeficiency virus type 1 packaging signal and major splice donor region have a conserved stable secondary structure.
... Comparison with other published human immunodeficiency virus type 1 sequences shows almost absolute nucleotide conservation in base-paired regions required to maintain this structure.. P[r] ... See full document
10
The Secondary Structure of the Human Immunodeficiency Virus Type 1 Transcript Modulates Viral Splicing and Infectivity
... the RNA substrates utilized for the RNA affinity chromatography (RAC) assays and the enzymatic probing of the RNA structures were generated by cloning into pBluescript II KS (Stratagene) a fragment ... See full document
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Sequences Downstream of the 5′ Splice Donor Site Are Required for both Packaging and Dimerization of Human Immunodeficiency Virus Type 1 RNA
... GA-rich RNA sequences at nt 325 to 336 sig- nificantly reduce levels of both viral RNA dimerization and ...GA-rich RNA sequence downstream of the SL3 structure participates in viral RNA ... See full document
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Determinants of human immunodeficiency virus type 1 envelope glycoprotein oligomeric structure.
... influenza virus hemagglutinin transmembrane protein HA2 ...trimeric structure of hemagglutinin is stabilized primarily by hydropho- bic intermonomer interactions in the N-terminal half of the extended ... See full document
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Role of the major homology region of human immunodeficiency virus type 1 in virion morphogenesis.
... The matrix protein of human immunodeficiency virus type 1 is required for incorporation of viral envelope protein into mature virions.. Mutations in the N-terminal region of human immuno[r] ... See full document
10
Use of Multiple Competitors for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma
... multiple RNA competitors differed in size but con- tained the same primer annealing sequences as those of the wild-type ...the RNA competitors also consisted of a poly(A) stretch, various ... See full document
7
Novel Ring Structure in the gp41 Trimer of Human Immunodeficiency Virus Type 1 That Modulates Sensitivity and Resistance to Broadly Neutralizing Antibodies
... the structure and function of gp41. Using the structure of Chan et ...trimeric structure is thus stabilized by a ring of amino acids Q655-Q553-V551 in ... See full document
11
Efficiency of Cell-Free and Cell-Associated Virus in Mucosal Transmission of Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus
... sealed human colon explants ...FIG 1 Colon explant ...Infected donor cells or cell-free virus was added to the medium to infect the sealed ...free virus particles did not penetrate ... See full document
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Effect of different donor cells on human immunodeficiency virus type 1 replication and selection in vitro.
... RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum, penicillin (250 U/ml), streptomycin (250 m g/ml), 10 mM HEPES (N-2-hydroxy- ethylpiperazine-N 9 -2-ethanesulfonic acid) buffer, and interleukin-2 ... See full document
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