[PDF] Top 20 Temporal Regulation of Herpes Simplex Virus Type 2 VP22 Expression and Phosphorylation

... of VP22 (Fig. 8A, lanes 5, 6, and 7). This result indicates that VP22 is very stable because it could be detected for at least 24 h after it was ...labeled VP22 in the D and C isoforms, and only ... See full document

... of phosphorylation sites in UL13 in HSV-2-infected ...physiological phosphorylation site(s) in UL13 in HSV-2-infected cells, we carried out a large-scale phosphoproteomic analysis of titanium ... See full document

... the temporal kinetics of ex- pression and on the respective dependencies on viral DNA ...Fig. 2, 3, and 6, UL24 was first detected at the same time as or later than the gB protein, the product of a ... See full document

... of VP22 on MTs observed during tran- sient expression, a time course of infection was carried out in Vero ...and VP22. Under these conditions VP22 was initially detected in the cell at 4 h ... See full document

... and VP22 from the wt virion tegu- ment in vitro was significantly enhanced by the combined pres- ence of Mg and ...(MW) VP22 in both the pellet and the soluble fraction of samples incubated with ATP and Mg ... See full document

... Herpes simplex virus 1 (HSV-1) protein ICP27 is a multifunctional regulatory protein that is posttransla- tionally modified by phosphorylation during viral ...kinase 2 (CK2) sites, and ... See full document

... line 2-2, which contains the wild-type ICP27 gene, were grown as described previously (14, 46, ...gene expression was driven by the HCMV pro- ... See full document

... (Fig. 2). The pattern of Sp1 phosphorylation in ...and 2) in that there was partial conversion to the phosphorylated ...gene expression profile of cells infected with wt HSV in the presence of ... See full document

... the phosphorylation state of ICP0: cellu- lar cyclin-dependent kinases (cdks) and the viral kinase, ...the expression of a late gene in HSV-in- fected cells; however, the phosphorylation state of ... See full document

... immunofluorescence was carried out with an antibody against ICP0. At early times in infection (2 h), there was little differ- ence in the characteristic nuclear localization patterns of ICP0 in either cell ... See full document

... of VP22 that appears to be af- fected by its phosphorylation status is its association with cel- lular MTs, a relationship that we initially demonstrated in cells transiently expressing VP22 ...of ... See full document

... and VP22, suggesting that VP22 itself may play a specific role in the accumulation of VHS protein in the cell ...⌬22 virus infections (21, 22), TABLE 1 Coding changes in the genome of our s17 ⌬ 22 ... See full document

... 1.5 expression is conserved among many strains of HSV and is not an artifact of genome rearrangement specific to strain ...mutant virus d22:GFP, in which the ICP22 gene is re- placed by a GFP ...and ... See full document

... for expression and secretion in Chinese hamster ovary cells. The expression vector contained a truncated form of HSV gB2 ending in Thr-696 of the predicted amino acid sequence (45) plus the sequence ... See full document

... The rate of formation of dCMP in cell extracts prepared from uninfected KB cells and KB cells infected with HSV-1 or HSV-2 was linear for at least 25 min and was proportional to extract [r] ... See full document

... Endo F converted the 74K peptide present in the monensin-treated cells into a 66K peptide, while the 110K to 116K peptide was only partially susceptible, as faster-migrating, diffused-re[r] ... See full document

... Several human trials were done with the subunit vaccines. A vaccine prepared from purified envelope glycoproteins (Merck, Sharp & Dohme) was studied in sexual partners. In a phase IIa trial the numbers of individuals ... See full document

... into the genome or caused by selection pressure on specific, functionally important amino acids. While conflicting signals caused by selection pressure are usually found accumulated at specific sites or epitopes, the ... See full document

... containing VP22/E7 protein or whether in- stead there is uptake of VP22/E7 protein when it is released after apoptosis or secreted from transduced ... See full document

... protein VP22 has been characterized with multiple traits including micro- tubule reorganization, nuclear localization, and nonclassical intercellular ...using herpes simplex virus type ... See full document