[PDF] Top 20 Testing the Hypothesis of a Recombinant Origin of Human Immunodeficiency Virus Type 1 Subtype E

... The human immunodeficiency virus type 1 (HIV-1) epidemic in Southeast Asia has been largely due to the emergence of clade E ...a recombinant lineage of ... See full document

... of origin and the year of sample collection were available for most sequences from the Los Alamos ...All subtype C sequences of ⱖ 8 kb were included in the final data ...270 subtype C sequences were ... See full document

... Human immunodeficiency virus type 1 (HIV-1) genetic diversity, due to its high evolutionary rate, has long been identified as a main cause of problems in the development of an ... See full document

... of human immunodeficiency virus type 1 (HIV-1) is a major problem to overcome in the development of an effective vaccine ...HIV-1 subtype B strains will be tested ... See full document

... pseudotyped virus that could enter target cells via GPR1, CCR1, CCR6, CXCR6, or RDC1 although SIVmac env clones could mediate entry via these CoRs ...subtypes E, A/D, A/C, CRF01_AE, or CRF02_AG HIV-1 ... See full document

... of human immunodeficiency virus type 1 (HIV-1) determines several viral properties ...on subtype-B viral strains, prevalent in North America and ...of ... See full document

... plus subtype G, monophyletic clustering of CRF02_AG alone, and monophyletic clustering of subtype G alone (see Materials and Methods for ...of subtype G, with only 10 trees in each run ...our ... See full document

... FIG. 1. HIV-1 sequences in cloning vectors for antiretroviral resistance ...ligase. Recombinant clones are cotransfected with p83-10 (6) into MT-2 cells to produce infectious ...ligase. ... See full document

... (v) Tris buffer-based detection. MTPs were blocked with antibody incubation buffer (100 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1% [wt/vol] blocking reagent [1096176; Roche Diagnostics Belgium]) for 10 min at room ... See full document

... colonies were again white due the inactivation of /3-galactosidase expression by the foreign insert. Plasmid DNA was prepared from suitable colonies by the 'mini-prep' procedure and analysed by restriction enzyme ... See full document

... six subtype B HIV-1 isolates (LAI, SF2, MN, OYI, JRFL, and JRCSF), three subtype D sequences (ELI, NDK, and Z2Z6), two subtype A sequences (UG037 and U455), the sequence of an A/G ... See full document

... “Subtype E” viruses represent A/E intersubtype recombi- ...“subtype E” ...“subtype E” genome, including the gag, pol, tat, rev, vpu, and 5 9 nef regions, is actually ... See full document

... Human immunodeficiency virus type 1 (HIV-1) displays an unusually high degree of genetic variability in ...circulating recombinant form (CRF) of virus has been ... See full document

... between subtype B and subtype C V3 sequences, it is not surprising that the positions that show covariation are among the most variable and discordant positions, which lie in the stem and turn do- mains ... See full document

... the recombinant constructions. The SHIVnef-fusion expresses a SIV–HIV-1 nef chimeric gene ...encephalomyocarditis virus, and HIV-1 nef as previously described ... See full document

... therefore may provide an alternative molecular genetic ap- proach to HIV-1 disease. Overall, this study supports the idea that a modified Cre could provide a reagent for therapy against HIV-1 infection. ... See full document

... Sera from rabbits that had been immunized with either native recombinant gpl60 or differently deglycosylated derivatives, after treatment with neuraminidase, a-mannosidase, or endo F-N g[r] ... See full document

... Although our binding experiments were not performed un- der equilibrium conditions, we have nevertheless estimated the concentrations and stoichiometries of the binding reaction components in an initial effort to define ... See full document

... sensitivity, 1 ml of plasma was ultracentrifuged for 1 h at 16,500 ⫻ g at 4°C, and the pellet was resuspended in 140 ␮l of HIV-negative human plasma before ... See full document

... From virus cultures, complete gag and env genes were PCR amplified and cloned as reported previously (27, 28) but using the Expand Long Template kit (Boehringer Mannheim, Indianapolis, ... See full document