[PDF] Top 20 Transcription of the Genomes of Type 1 and Type 3 Reoviruses
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Transcription of the Genomes of Type 1 and Type 3 Reoviruses
... To look into this question, BHK21 cells were infected with type 1 virus and the ssRNA formed between 10 and 12 hr was labeled, extracted, and analyzed.. The results Fig.[r] ... See full document
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Human T-Lymphotropic Virus Type 1 Transcription and Chromatin-Remodeling Complexes
... Lane 1, input histones used for reconstitution arrays (2.5 g); lanes 2 and 3, reconstituted DNA molecules before and after exposure to the glycerol ... See full document
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Bovine Papillomavirus Type 1 Genomes and the E2 Transactivator Protein Are Closely Associated with Mitotic Chromatin
... viral genomes are attached to mitotic chroma- tin indirectly via the E2 protein and E2 DNA binding ...viral genomes are segregated to daughter cells. Viral genomes that replicate as extrachromosomal ... See full document
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Characterization of Intracellular Reverse Transcription Complexes of Human Immunodeficiency Virus Type 1
... isolated 1, 4, and 7 h after acute infection were competent for DNA synthesis in ...(2, 3, 4, ...about 1% of the MA content of an intact virion), or to the inability of the monoclonal antibody to ... See full document
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Permissive Sense and Antisense Transcription from the 5′ and 3′ Long Terminal Repeats of Human T-Cell Leukemia Virus Type 1
... antisense transcription in that complex events occurring during infection and involving multiple HTLV-1 genes likely influence the abundance of viral RNA (43– ...sense transcription in all ...(lane ... See full document
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SIRT1 Suppresses Human T-Cell Leukemia Virus Type 1 Transcription
... HTLV-1 transcription by ...groups 1 and 4 received dimethyl sulfoxide (DMSO) ...groups 3 and 4 is statistically significant by two-tailed Student’s t test, with a P value equal to ...(groups ... See full document
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The importance of the A-rich loop in human immunodeficiency virus type 1 reverse transcription and infectivity.
... collected 3 and 8 h later, ...and 1 mg of protease K per ...(Fig. 1), which generated a 155-bp (2)ssDNA product that was analyzed on 6% nondenaturing acrylamide ... See full document
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Restoration of Wild-Type Infectivity to Human Immunodeficiency Virus Type 1 Strains Lacking nef by Intravirion Reverse Transcription
... Proviral DNA was detected by traditional PCR using the following primer set to detect strong-stop DNA: forward primer 5⬘-GGC TAA CTA GGG AAC CCA CTG CTT and reverse primer 5⬘-CTG CTA GAG ATT TTC CAC ACT GAC, which ... See full document
7
Human T-Cell Leukemia Virus Type 1 (HTLV-1) bZIP Factor Requires Cellular Transcription Factor JunD To Upregulate HTLV-1 Antisense Transcription from the 3′ Long Terminal Repeat
... virus type 1 (HTLV-1) results in a variety of diseases including adult T-cell leukemia (ATL), a fatal malignancy characterized by the uncontrolled proliferation of virally infected CD4 ⴙ T ...the ... See full document
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Human T-Cell Leukemia Virus Type 1 (HTLV-1) bZIP Protein Interacts with the Cellular Transcription Factor CREB To Inhibit HTLV-1 Transcription
... virus type 1 (HTLV-1) retrovirus encodes several proteins that are unique to the virus within its 3 ⴕ -end ...HTLV-1 transcription through its ability to interact with specific ... See full document
11
YY1 represses human papillomavirus type 16 transcription by quenching AP-1 activity.
... positions 1 16 to 1 80, while the two constructs with the prefix p103 contain the HPV promoter sequence from genomic positions 1 16 to 1 103, which includes YY1 site ...of 1, and the ... See full document
11
Repression of host RNA polymerase II transcription by herpes simplex virus type 1.
... approximately 3 h ...cellular transcription factors such as Sp1 (re- viewed in reference ...why transcription occurs efficiently on viral DE and L gene promoters and not on cellular gene promoters ... See full document
10
Cell Cycle-Regulated Transcription by the Human Immunodeficiency Virus Type 1 Tat Transactivator
... vitro transcription reaction mixture containing 25 g of ...every 3 h, and the cells were used to make whole-cell extracts for in vitro transcription or Western blot ...G 1 ; 0, 3, 6, ... See full document
9
Phosphorylation of Transcription Factor Sp1 during Herpes Simplex Virus Type 1 Infection
... for 1 h at 37°C with ...of 3 liters, and the infection was allow to proceed for a further 12 ...general transcription factors and RNA ...of transcription factors were optimized to produce ... See full document
7
Cell Factors Stimulate Human Immunodeficiency Virus Type 1 Reverse Transcription In Vitro
... on a 14%-to-28% OptiPrep velocity gradient, and reverse tran- scription products were detected in the fractions. When lysate was mixed with detergent-treated virions, a complex associated with strong-stop reverse ... See full document
13
Targeting Tat Inhibitors in the Assembly of Human Immunodeficiency Virus Type 1 Transcription Complexes
... with 1% formaldehyde for 5 min at room temperature, and the reaction was stopped by the addition of glycine (150 ...immunoprecipitation, 1 mg of protein extract was precleared for 2 h with 40 l of a ... See full document
13
Identification of Carbohydrate-Binding Domains in the Attachment Proteins of Type 1 and Type 3 Reoviruses
... 1, is responsible for strain-specific patterns of viral tropism in the murine central nervous system and receptor binding on cultured ... 1 protein consists of a fibrous tail domain proximal to the ... See full document
8
Sequences in the 5′ and 3′ R Elements of Human Immunodeficiency Virus Type 1 Critical for Efficient Reverse Transcription
... reverse transcription was determined using an endogenous reverse transcriptase assay in which nu- cleotides and small amounts of detergent are incubated with sucrose-purified virion particles ...or 3 ⴕ R ... See full document
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The integrase interactor 1 (INI1) proteins facilitate Tat mediated human immunodeficiency virus type 1 transcription
... INI1 is dispensable for HIV-1 transduction. a. Inhibition of ini1 expression by shRNA-producing lentiviral vector. Ethidium bromide-stained agarose gel analysis of RT-PCR amplification products of cytoplasmic RNA ... See full document
6
Role of Vif in human immunodeficiency virus type 1 reverse transcription.
... U3( 1 ) probe detects initial plus-strand DNA products imme- diately adjacent to the 3 9 ...gag/pol( 1 ) probe detects plus-strand DNA products synthesized after the second tem- plate switch or ... See full document
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