[PDF] Top 20 Use of a Genus and Species Specific Multiplex PCR for Identification of Enterococci
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Use of a Genus and Species Specific Multiplex PCR for Identification of Enterococci
... and identification of enterococci by using the traditional phenotypic differentiation can be a tedious pro- cess requiring numerous ...20 species can be identified using these methods, tests are ... See full document
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A Simple and Efficient Multiplex PCR Assay for the Identification of Mycobacterium Genus and Mycobacterium tuberculosis Complex to the Species Level
... template. PCR am- plification was performed in an automated thermal cycler (PTC-100 Programmable Thermal Controller, MJ Research, ...of PCR products was performed by electrophoresis in ethidium bromide ... See full document
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Multiplex PCR Method for Species Identification of Coagulase Positive Staphylococci
... CoPS species in veterinary clinical ...staphylococcal species and devised a multiplex-PCR (M-PCR) method for species identification of CoPS by targeting the nuc gene ... See full document
5
Detection and Identification of Mycobacteria by Amplification of the Internal Transcribed Spacer Regions with Genus and Species Specific PCR Primers
... of PCR assays that target the internal transcribed spacer (ITS) region for identifying mycobacteria at the species ...and species-specific ITS sequences of 33 species of mycobacteria ... See full document
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A multiplex PCR for rapid identification of Brassica species in the triangle of U
... genome specific markers, C1 (157 bp, located on chromosome C01 of ...genome specific markers were successfully ...the use of two A genome markers derived from separate A chromo- ...diverse ... See full document
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Use of tuf Sequences for Genus Specific PCR Detection and Phylogenetic Analysis of 28 Streptococcal Species
... streptococcal species was obtained by sequencing amplicons generated using broad-range PCR ...Streptococcus-specific PCR primers and to perform phylogenetic ...the PCR assay was ... See full document
10
Development of a PCR Assay for Identification of Staphylococci at Genus and Species Levels
... API identification systems (bioMe´rieux Vitek) (1, 21) and automated systems such as the MicroScan system (Dade Behring ...of PCR-based methods for the species-specific detection of ... See full document
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Simultaneous Detection of Helicobacter Genus and Helicobacter pylori Species using a Multiplex PCR Method
... Helicobacter genus members are fastidious and very slow growing microorganisms ...and specific PCR assays has alleviated problems typically associated with identification of microorganisms ... See full document
5
Multiplex PCR assay for immediate identification of the infecting species in patients with mycobacterial disease
... mycobacterial species in immunosuppressed patients are increasingly being ...mycobacterial species can usually be identified only after culture of the or- ganism, a time-consuming ...infecting ... See full document
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Identification of Mycobacterium tuberculosis clinical isolates in Bangladesh by a species distinguishable multiplex PCR
... multiple PCR reactions were needed) [2,3], result interpretations seemed to be compli- cated ...additional PCR for the detection of RD1 can distinguish BCG from clinical ...MTC species is ... See full document
7
Primer Design for the Identification of Ten Oral Actinomyces Species Using Multiplex PCR
... for species identification using PCR [16]. Moreover, multiplex-PCR is a rapid tool that allows for the simultaneous amplification of more than one sequence of target DNA in a single ... See full document
6
Rapid Detection and Identification of Human Adenovirus Species by Adenoplex, a Multiplex PCR Enzyme Hybridization Assay
... the PCR-based assays recently described in the ...described species- specific multiplex assay they had poor amplification of sero- types 11, 21, 34, and 35 of species B, yet their assay ... See full document
5
Rapid and Accurate Identification of Human Associated Staphylococci by Use of Multiplex PCR
... definitive species identifica- ...simple multiplex-PCR (M-PCR) for species identification of human-associated staphylococci, which were as follows: Staphylococcus aureus, ...the ... See full document
5
Species Specific Identification of Human Adenoviruses by a Multiplex PCR Assay
... type-specific identification of Ads, a provisional group- ing is usually first obtained by determining the agglutination patterns of the isolate with human and animal erythrocytes (19, ...cases, ... See full document
8
Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group
... To see if a more recent melt-curve approach could more accurately identify the small difference in T M between these three species an assay based on HRM was developed. Like MCA the running cost of HRM is low (US ... See full document
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Development and Validation of a Multiplex PCR for Detection of Scedosporium spp in Respiratory Tract Specimens from Patients with Cystic Fibrosis
... to PCR am- plification, due to an insufficient amount of ...study. Species identification is further important, as species-specific clinical associations and antifun- gal ... See full document
5
Evaluation of a Multiplex PCR Test for Simultaneous Identification and Serotyping of Actinobacillus pleuropneumoniae Serotypes 2, 5, and 6
... the species and the serotype. Various PCR tests have been developed for species identification of ...these PCR tests is that the primers used for amplification are intended to be ... See full document
6
A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand
... six species available in GenBank (Table 2); the ITS2 sequence of ...the PCR products and respective melt- ing temperatures (Tm) are presented in Table ...some species having similar individual size ... See full document
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Picky eaters are rare: DNA based blood meal analysis of Culicoides (Diptera: Ceratopogonidae) species from the United States
... Morphological identification of the species within the C. variipennis species complex is not always straightfor- ward as there are few, if any, discrete synapomorphies that define the ...each ... See full document
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Development of a Multiplex PCR for the Detection of asa1, gelE, cylA, esp, and hyl Genes in Enterococci and Survey for Virulence Determinants among European Hospital Isolates of Enterococcus faecium
... areth, Belgium). After cell wall and protein digestion, the plugs were digested overnight with 30 U of SmaI (MBI Fermentas, St. Leon-Rot, Germany) at 25°C. PFGE was performed with a 1% agarose gel by using a CHEF Mapper ... See full document
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