[PDF] Top 20 Vesiculovirus Neutralization by Natural IgM and Complement

... ABSTRACT Because of its very low human seroprevalence, vesicular stomatitis virus (VSV) has promise as a systemic oncolytic agent for hu- man cancer therapy. However, as demonstrated in this report, the VSV infectious ... See full document

... C2 IgM mAbs that we utilize in this study have been previously described: B4 recognizes a post-translational modification of annexin IV [9] and C2 recognizes a subset of phospholipids ...of IgM Abs of B4 ... See full document

... that natural IgM antibodies bind to HSV-1 and HSV-2 raises interesting ...coded IgM antibodies may have evolved to control HSV, al- lowing natural antibodies to function with the ... See full document

... 2. Xu Z, Tian J, Smith JS, Byrnes AP. 2008. Clearance of adenovirus by Kupffer cells is mediated by scavenger receptors, natural antibodies and complement. J Virol 82:11705–11713. ... See full document

... of natural IgM was observed by Zola et ...abundant complement components to be present at the mucosal surface of the nasopharynx, thereby pro- viding selective ... See full document

... the complement system reduces the efficacy of systemic applications of virothera- pies (24, ...by natural IgM antibodies present in human ...classical complement pathway, which in turn resulted ... See full document

... and complement. Although natural an- tibodies tend to have low affinity and low abundance, they can have profound biological effects in inhibiting both viral infec- tion and viral gene ...Polyreactive ... See full document

... In studies of natural infection, the CF test, ELISA, and neutralization assay have all been sensitive in detecting seroresponses to rotavirus.. The CF test detected antibody rises in 75 [r] ... See full document

... PIV5, natural variants that differ in fusion activity have been de- scribed ...for neutralization as described in the legend to ...in neutralization of WT and G3A viruses that were derived from A549 ... See full document

... The complement system is a critical component of the innate immune response that all animal viruses must face during natural ...of complement C3-derived polypeptides on virion ...of complement ... See full document

... with a number of recombinant HCV proteins. However, inves- tigation into the functional role of the antibodies has not been convenient due to the lack of a dependable HCV replication system. Disease-specific mimotopes ... See full document

... Ihle and Lazar (14) have attempted to improve the neutralization test for mouse antibodies against the endogenous ecotropic virus of AKR mice by using freshly harvested, unfrozen virus w[r] ... See full document

... Complement-Mediated Neutralization of Dengue Virus Requires Mannose-Binding Lectin Panisadee Avirutnan, a,b Richard E. Hauhart, a Mary A. Marovich, c Peter Garred, d John P. Atkinson, a,e,f and Michael S. ... See full document

... of complement-fixing sites per erythrocyte formed by antibody was determined for each of the various preparations by the Cla fixation and transfer ...either IgM or IgG antibodies, and dose- response curves ... See full document

... ment, if activated by antibody, can neutralize viruses by at least two mechanisms, i.e., direct lysis and physical inter- ference with infection by deposition of complement proteins on t[r] ... See full document

... and vesiculovirus genera, has recently emerged as human pathogen that is associated with a number of outbreaks in different parts of ...prototype vesiculovirus, Vesicular Stomatitis Virus, it could be ... See full document

... of complement caused a >50% loss of anti-B5 IgG2a protection, directly establishing the importance of complement for protection against the EV ...by complement-fixing anti-B5 ...via complement ... See full document

... vitro complement-mediated neutral- ...inhibit complement-mediated neutralization of vaccinia vi- rus virions may result in the complement system helping the host regain control of a vaccinia ... See full document

... light beam arrays in the horizontal x and y axes. The hardware detects beams broken by animal movements to determine the location within the cage. In all cages, mice were exposed to identical environmental condi- tions: ... See full document

... every IgM anti-erythrocyte antibody molecule in an antibody preparation was shown to fix ...60 IgM complement-fixing sites per erythrocyte were required for the immune clearance of ... See full document