[PDF] Top 20 Viral Determinants of Polarized Assembly for the Murine Leukemia Virus

... the murine leukemia virus (MLV) as a model retrovirus, we have previously shown that interaction between viral envelope (Env) and receptor directs viral assembly to cell-cell ... See full document

... Our results suggest that the 5' half of the SU gp70 coding region of the env gene of PVC-211 MuLV contains the determinants responsible for the induction of pathological changes in the C[r] ... See full document

... of virus assembly and virion budding (34, ...for virus budding (21, 22, ...restore viral assembly to an M-MuLV PPPY ...virion assembly. The RSV and M-MuLV L-domains restored the ... See full document

... Gag VLP cores. We found that these particles were disrupted to a significant extent simply by the detergent treatment that was used to expose the capsid to RNase. These findings suggest that the basic region on the ... See full document

... Moloney murine leukemia virus (Mo-MuLV) which package normal levels of genomic RNA but are nevertheless noninfectious ...the viral life cycle in addition to their function in RNA ...released ... See full document

... the viral genomic RNA to the 3⬘ end of the RNA using the two identical R regions at the ends of the viral ...of viral replication, retroviral reverse transcriptases (RTs) have evolved to possess low ... See full document

... hexamer assembly is not known. Since our mutants were defective for viral release, most of the Gag protein in cells expressing them was not proteolytically processed, and the lattice formed by such Gag ... See full document

... The order of addition of the LTR substrate with respect to IN 1–105 or IN 106–404, surprisingly, altered the preference for the two-end concerted products. The majority of the prod- ucts formed after preincubation of the ... See full document

... the viral RNA genome, reverse transcription, and ...infectious viral clone, was based, at least in part, on our previous analysis of HIV-1 assembly and IN ...that viral proteins could be ... See full document

... the viral genes. (B) Western blot of virus lysates from ET cells transfected with the indicated MoMLV ...cell-associated viral proteins are not provided because meaningful quantification of intracel- ... See full document

... virion assembly and release, provided that it is coupled with a defect in the viral PR ...in virus produc- tion observed in other studies (14, 21, 25, 47) may actually be due, at least in part, to ... See full document

... of cell culture supernatant. The use of chronically infected cells precludes reinfection with virion entry and ensures that only late phases of the viral cycle were observed. For cell morphology analysis, the ... See full document

... In vitro CAT activity of extracts derived from HeLa cell cultures that transiently expressed transfected plasmids containing the enhancer-proficient SV40 early region promoter SV2, the e[r] ... See full document

... Cellular ELISA. Antibody directed against MuLV proteins was detected with a cellular enzyme-linked immunosorbent assay (ELISA) developed in our labo- ratory. NIH 3T3 fibroblast cells (American Type Culture Collection, ... See full document

... of virus-like particles (VLP) of a gammaretrovirus, the xenotropic murine leukemia virus (MLV)-related virus ...the assembly of ⌬ ProCANC or ⌬ 10CANC into immature-like spherical ... See full document

... in viral replication. The virion particle, contain- ing the viral RNA genome, is composed principally of a large array of Gag multimers (reviewed in reference ...with viral RNA, and nuclear magnetic ... See full document

... ciency virus type 1 (HIV-1), encodes three polyproteins: the Gag precursor of the viral structural proteins (matrix, capsid, and nucleocapsid), the Pol precursor of the viral enzymes (pro- tease, ... See full document

... One possible explanation for the apparent discrepancy between the accumulation of the cellular virions and the unchanged level of the total cytoplasmic viral RNA in IF-treated cells is t[r] ... See full document

... We also describe here that the glycosyl- Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Leukemic cells of AKR mice contain as constituents of their membranes the murin[r] ... See full document

... Cell lines used in this study Cell line B559 Mouse fibroblast Mouse, transformed fibroblast Mouse fibroblast Mouse fibroblast Mouse fibroblast Mouse fibroblast Mouse fibroblast Mouse fib[r] ... See full document