[PDF] Top 20 In Vitro Processing of Herpes Simplex Virus Type 1 DNA Replication Intermediates by the Viral Alkaline Nuclease, UL12

... that alkaline nuclease does not completely degrade well ...well DNA is the first published DNA substrate that is not digested to completion by this ...As UL12 exhibits no sequence ... See full document

... The herpes simplex virus (HSV) genome replicates in the nucleus of an infected host cell, resulting in the production of longer-than-unit-length head-to-tail concatemers of viral ...infectious ... See full document

... HSV-1 UL12 gene encodes both full-length UL12 and ...at UL12 codon 127 (4, ...mitochondrial DNA degradation, but the biological significance of mitochondrial DNA degradation in ... See full document

... with herpes simplex virus type 1 (HSV-1) results in the rapid elimination of mitochondrial DNA (mtDNA) from host ...the viral alkaline nuclease ... See full document

... The herpes simplex virus (HSV) type I alkaline nuclease, UL12, has 5=- to-3= exonuclease activity and shares homology with nucleases from other mem- bers of the ... See full document

... in herpes simplex virus type 1 (HSV-1)-infected cells: A (empty), B (in- termediate), and C (full) (15, ...either DNA or ...38). Replication of viral ... See full document

... active DNA replication were identified by the incorporation of 5⬘ bromo-2-deoxyuridine ...antibody. Viral DNA replication compart- ments in nontransfected or transfected cells were ... See full document

... HSV DNA replication occur by theta replica- tion structures initiated at one or more of the three origins of ...bacteriophage DNA replication (36, ...further DNA replication, and ... See full document

... The nuclease activities of UL12 have been thought to be im- portant for UL12 in promoting viral replication in cell cultures, based on reports showing that (i) UL12 null mutant ... See full document

... replicated viral DNA prior to its packaging into ...of viral polypeptides which play a role in this encapsidation process have been identified (reviewed in refer- ence 23), the actual protein which ... See full document

... HSV-1 replication but not gene expres- ...or DNA replication, we examined in- fections in cells treated with the drug phosphonoacetic acid (PAA), a specific inhibitor of the viral ... See full document

... Location of abundant early HSV-1 mRNA' Size RNA RNA Major restriction fragNen to RN bands hybridizing Restriction fragment band hybridizing to 3' end probe of 2 h p.i.. b The sizes shown[r] ... See full document

... and 10% vol/vol tryptose phosphate broth Difco on 314 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Temperature-sensitive mutants of herpes simplex virus type 1 repre[r] ... See full document

... herpesvirus DNA. By introducing single copies of test packaging signals into an HSV-1 genome lacking the normal resident copies, we have overcome one of the major drawbacks of the previously used amplicon ... See full document

... of DNA molecules ranging in size up to 9 ...of DNA chains largely per- ...unit-length DNA molecules ...circle replication of the circular plasmid, is the same as that previously observed with ... See full document

... full-length DNA chains that are essentially free of nicks ...of replication products migrates in the first di- mension at a position consistent with their being mAAV ... See full document

... Identification of the Oscar elements. As an alternative means of identifying specific flanking sequences that can enhance the function of the minimum core origin contained in pOS 80 (80), oriS-flanking sequences were ... See full document

... Herpes simplex virus (HSV) exhibits altered gene regulation in neuronal compared to nonneuronal ...of DNA synthesis at the viral origins of replication (oriS and oriL) is a ... See full document

... replicative intermediates. The HSV-1 alkaline nuclease is a possible candidate for a debranching ...stein-Barr virus (30). Alkaline nuclease has been found in a protein ... See full document

... several viral proteins, such as the Rabies virus P-protein and chicken anemia virus VP3, regulate their NLS/NES activity via phosphor- ylation ... See full document