Course director: DR.TÍMEA BERKI, professor
Department of Immunology and Biotechnology 4 credit ▪ exam + progress grade ▪ Basic module ▪ spring semester ▪ recommended semester: 2
Number of hours/semester: 28 lectures + 28 practices + 0 seminars = total of 56 hours Course headcount limitations (min-max.): min. 5 – max. 20
Prerequisites: none Topic
Novel antibodies against receptors, protein kinase inhibitors, and antisense oligonucleotides targeting both signal transduction and gene expression will predominate the therapeutic approaches in the coming decades. The course focus on the therapeutic potential for targeting cell signaling mechanisms with particular attention to cancer therapies and inflammatory signaling pathways as well as immunomodulation. General concepts of inter-and intracellular signal transduction: receptor-mediated signal transduction, cell surface receptors, steroid hormone and nuclear receptors and their cytoplasmic signal transduction and nuclear responses, apoptotic cell signaling will be discussed together with the novel approaches to drug discovery in signal transduction. Internationally known experts of the field guest lecturers from Hungarian universities are contributing in the course.
Conditions for acceptance of the semester
Maximum 3 absences allowed
Making up for missed classes
-
Reading material
1. Gerhard Krauss: Biochemistry of Signal Transduction and Regulation, Wiley, Finkel, Toren (ed.) 2. Gutkind, J. Silvio (ed.): Signal Transduction and Human Disease, John Wiley & Sons, Inc.
3. Frederick Marcus: Bioinformatics and Systems Biology: Collaborative Research and Resources, Springer, 2008
Lectures
1 Introduction, overlap with other disciplines Dr. Berki Timea
2 Overview of extracellular signaling Dr. Berki Timea
3 Families of extracellular receptors Dr. Boldizsár Ferenc
4 Second messengers (cAMP) Dr. Boldizsár Ferenc 5 Receptor tyrosine kinases
Dr. Boldizsár Ferenc 6 The Ca++ signal
Dr. Boldizsár Ferenc 7 Transcription factors
Dr. Berki Timea
8 Signaling in the specific immune system: B cell signaling. Dr. Berki Timea
9 T cell activation and signaling Dr. Berki Timea
10 Fcgamma and Fcepsilon Receptor signaling Dr. Berki Timea
11 Tyrosine kinase-linked receptors I.: cytokine/chemokine signaling Dr. Boldizsár Ferenc
12 Tyrosine kinase-linked receptors II.: growth factors Dr. Boldizsár Ferenc
13 Receptors with intrinsic enzymatic activity (insulin, growth factors). Dr. Berki Timea
14 G-protein-linked receptors (epinephrine, serotonin, glucagon). Dr. Berki Timea
39 15 Signaling in tumor cells (EGF-R, Her-2R, adhesion molecules).
Dr. Berki Timea 16 Apotosis signaling
Dr. Berki Timea
17 Ion-channel receptors (acetilcholine receptor). Cell-surface hormone receptors Dr. Berki Timea
18 Intracellular/nuclear receptor signaling (steroid hormones and thyroxin). Dr. Boldizsár Ferenc
19 Non-genomic steroid hormone signaling pathways. Dr. Boldizsár Ferenc
20 Signaling in the innate immune system: CR and TLR signaling Dr. Berki Timea
21 Receptor interactions, signaling cross-talk Dr. Berki Timea
22 Wnt receptor signaling. Dr. Pongrácz Judit
23 Role of signal transduction in cell differentiation Dr. Pongrácz Judit
24 Adhesion molecule signaling Dr. Berki Timea
25 Role of adhesion molecules in tumor progression Dr. Berki Timea
26 Signaling in the nervous system Dr. Berki Timea
27 Pharmacological influence of the signaling. Dr. Pethő Gábor
28 Pharmacological influence of the signaling. Dr. Pethő Gábor
Practices
1 Measurement of intracellular Ca++ level using flow cytometry 2 Measurement of intracellular Ca++ level using flow cytometry 3 T cell activation with anti-CD3; mast cell activation through FceR. 4 T cell activation with anti-CD3; mast cell activation through FceR.
5 Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. 6 Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway.
7 Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
8 Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
9 Detection of transcription factor activation 10 Detection of transcription factor activation
11 FcepsilonR signaling elicited biological responses in vivo (mouse model) and in vitro (mast cell model). 12 Passive cutaneous anaphylaxis, degranulation test
13 Passive cutaneous anaphylaxis, degranulation test 14 Signaling pathways of apoptosis (intrinsic and extrinsic). 15 Signaling pathways of apoptosis (intrinsic and extrinsic).
16 AnnexinV/propidium-iodode staining; detection of activated Caspase-8, -9, -3; analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
17 AnnexinV/propidium-iodode staining; detection of activated Caspase-8, -9, -3; analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
18 Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. 19 Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways.
20 Translocation of the glucocorticoid-receptor into the nucleus and the mitochondrium (confocal microscopy) in different cell lines.
21 Translocation of the glucocorticoid-receptor into the nucleus and the mitochondrium (confocal microscopy) in different cell lines.
22 Gene-expression changes induced by the GC signaling (PCR). 23 Gene-expression changes induced by the GC signaling (PCR). 24 Immunhistological detection of tumor related growth factor receptors 25 Immunhistological detection of tumor related growth factor receptors 26 Consultation 27 Consultation 28 Practical exam Seminars Exam topics/questions - Participants
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OMA014 I
MMUNOLOGYCourse director: DR.PÉTER NÉMETH, professor
Department of Immunology and Biotechnology 3 credit ▪ exam + progress grade ▪ Basic module ▪ spring semester ▪ recommended semester: 2
Number of hours/semester: 14 lectures + 28 practices + 0 seminars = total of 42 hours Course headcount limitations (min-max.): min. 5 – max. 50
Prerequisites: none Topic
The subject comprises major immunological processes, their cellular and molecular components, discusses the regulation of a physiological immune response. The course also briefly summarizes basic processes of immune-pathologies (i.e. autoimmunity, tumor- immunity, etc.). The practices allow for the participants to become familiar with standard and modern immunological techniques.
Conditions for acceptance of the semester
Participation on the lectures. No more as 3 absences.
Making up for missed classes
No
Reading material
Kenneth M. Murphy, Paul Travers, and Mark Walport: Immunobiology 7 PB (Janeway’s Immunobiology) (Immunobiology: The Immune System (Janeway))
Thomas J. Kindt, Barbara A. Osborne, and Richard A. Goldsby: Kuby Immunology (Kindt, Kuby Immunology) H. Chapel: Essentials of Clinical Immunology, 5th edition, Blackwell Publishing, 2008
Lectures
1 Introduction, historical overview, main aspects and perspectives in the theoretical and practical immunology - immunobiology. General characteristics of the immune machinery.
Dr. Németh Péter
2 Composition of the immune system: organs, tissues and cells. Dr. Németh Péter
3 Antigen recognition molecules. Definition of the antigen. Dr. Németh Péter
4 Molecular structures and main functions of antibodies, B cell - and T cell receptors. Antigen-antibody reactions. Dr. Németh Péter
5 Recognition by MHC. Molecular structure of MHC, subclasses, genetics, functions. Dr. Németh Péter
6 Antigen presentation and MHC restriction. Superantigens. Dr. Németh Péter
7 Communication between cellular components of the immune system. (I.): Co-receptors and adhesion molecules. Dr. Németh Péter
8 Communication between cellular components of the immune system. (I.I): Main characteristics of the cytokines, chemokines, interferons, growth factors and their role in the immune regulation.
Dr. Berki Timea
9 Complement system (molecular components, activation pathways). Dr. Berki Timea
10 Main components of the innate immune system. Mechanism of the inflammation. Dr. Berki Timea
11 B cell development in the bone marrow. Maturation stages and environmental regulators. Dr. Balogh Péter
12 T cell development in the thymus. Maturation stages and environmental regulators. A brief overview of immune responses. Lymphocyte recirculation, role of the microenvironment.
Dr. Berki Timea
13 The central phase of the immune response, cellular interactions, B cell activation, T-B cell cooperation, antibody production, the role of adhesion molecules and cytokines.
14 Suppression of immune response. Systemic and local immunity. Mucosa and skin associated immune system. Dr. Németh Péter
Practices
1 Fehérvérsejtek izolálása ficoll grádiens centrifugálással.
2 Fehérvérsejtek vizsálata citotcentrifugálással és May-Grünwald Giemsa festéssel. 3 Immuncytokémiai reakciók.
4 CD3 és CD19 immuncytokémiai reakció végzése fehérvérsejteken. 5 Immunhiszkokémiai reakció alaptíusai.
6 Immunhisztokémiai reakcióe kryosztátos nyirokcsomó lép metszeteken. 7 Áramlási cytometria elméleti alapjai.
8 Áramlási cytomtriás jeleölés izolált fehérvérsejteken. 9 Áramlási cytometriás mérés.
10 Áramlási cytometriás mérési eredmények kiértékelése. 11 Microbead technika alkalmazásának lehetőégei.
12 Cytokin meghatározás microbead technikával, áramlási cytométerrel. 13 Immunszerológiai módszerek.
14 Immunprecipitáció, radiális immundiffúiós és Ouchterlony technikával antigén-antitest reakció végzése. 15 Immunelektroforézis. Immunelektroferetikus minták kiértékelése.
16 Hemagglutináció, hemagglutináció gátlás.
17 Hormon meghatározás heamagglutinációs technikával. 18 ELISA, RIA, FIA tehcnika .
19 Ellenanyag és antigén meghatározás ELISA technikával. 20 ELISA mérési eredmények kiértékelése, standardizálás. 21 In vivo immuntechnikák elméleti és technikai alapjai. 22 Fagocitósis teszt.
23 Fagocita funkciók vizsgálata humán és egér makrofág/monocita sejteken. 24 HLA meghatározás. Kevert limfocita reakció
25 Autoantitest mérések technikája.
26 Vércsoport meghatározás technikai szabályai.
27 Vércsoport meghatározás végzése humán vérmintákon. 28 Az immunolóiai labortechnikák minőségbiztosítása.
Seminars
Exam topics/questions
Multiple choice questions are prepared according to the lectures. See more details on www.immbio.hu
Participants
43
OMA015 M
ICROBIOLOGYCourse director: DR.JÚLIA SZEKERES, professor
Department of Medical Microbiology and Immunology
3 credit ▪ exam + progress grade ▪ Basic module ▪ spring semester ▪ recommended semester: 2
Number of hours/semester: 14 lectures + 28 practices + 0 seminars = total of 42 hours Course headcount limitations (min-max.): min. 1 – max. 200
Prerequisites: none Topic
During introduction the subject and history of medical microbiology and its place in medicine is discussed. The morphology, physiology and genetics of microbes, as well as the methods of disinfection and the theoretical aspects of antimicrobial therapy are the subjects of lectures on general microbiology. The host-parasite interactions involved in the pathogenesis of various infections, as well as the mechanisms of host defense, the possibilities of immunoprophylaxis will also be covered.
The most important bacterial, viral, fungal and parasitic infections of humans will be discussed, with diagnostic algorithms according to organ system infections and possible therapeutic measures including antimicrobial agents.
A special emphasis will be given to topics and methods (e.g. phages, molecular biological methods) which have a higher relevance in biotechnology.
Conditions for acceptance of the semester
Absences of 25% from the total number of lessons are accepted
Making up for missed classes
There is no possibility to catch up missed lessons
Reading material
1. Warren Levinson: Medical Microbiology and Immunology
2. Alexander N. Glazer and Hiroshi Nikaido: Microbial Biotechnology: Fundamentals of Applied Microbiology 3. Wayne William Umbreit: Advances in Applied Microbiology, Academic Press, 2005
Lectures
1 Orientation, bacterial cell structure Dr. Palkovics Tamás
2 Physiology of bacteria Dr. Palkovics Tamás
3 Bacterial genetics
Dr. Tigyi Zoltán
4 Pathogenicity and virulence, endo- and exotoxins Dr. Kocsis Béla
5 Desinfection and antimicrobial therapy I. (antimicrobial agents) Dr. Mestyán Gyula
6 Antimicrobial therapy II. (Alternative antimicrobials: phages, metagenomics, bioactive materials Dr. Schneider György
7 Virology I.
Dr. Szereday László
8 Virology II.
Dr. Szereday László
9 Bacterial pathogens and diseases of humans I.
Dr. Tigyi Zoltán
10 Bacterial pathogens and diseases of humans II. Dr. Mestyán Gyula
11 Immunology in microbiology: defence mechanisms against microbial pathogens
Dr. Szereday László
12 Immunology in microbiology: vaccines Dr. Palkovics Tamás
13 Fungal and parasitic infections of humans Dr. Mestyán Gyula
14 Rapid microbiological methods, biosensors Dr. Schneider György
Practices
1 Orientation, microscopy in microbiology, staining methods 2 Orientation, microscopy in microbiology, staining methods 3 Culture of bacteria
4 Culture of bacteria
5 Biochemical test in the identification of bacteria 6 Biochemical test in the identification of bacteria 7 Antibiotic resistance testing
8 Antibiotic resistance testing
9 Serological tests in medical microbiology 10 Serological tests in medical microbiology
11 Molecular biological methods in medical microbiology 12 Molecular biological methods in medical microbiology 13 Diagnostic of viral infections
14 Diagnostic of viral infections 15 Phages, transformation 16 Phages, transformation
17 Bacterial diagnostic of wound secrete, haemoculture 18 Bacterial diagnostic of wound secrete, haemoculture 19 Bacterial diagnostic of urinary tract infections 20 Bacterial diagnostic of urinary tract infections 21 Bacterial diagnosis of respiratory tract infections 22 Bacterial diagnosis of respiratory tract infections 23 Bacterial diagnostic of gastrointestinal diseases 24 Bacterial diagnostic of gastrointestinal diseases 25 Diagnostic of anaerobic bacteria
26 Diagnostic of anaerobic bacteria
27 Diagnostic of mycologic and parasitic infections 28 Diagnostic of mycologic and parasitic infections
Seminars
Exam topics/questions
The students have an oral exam where they pick two questions, one practical and one theoretical. The questions cover the topics of the lectures and practices. For passing the exam the students should reach at least a mark 2 (satisfactory) in both questions.
Participants
Dr. Kocsis Béla (KOBHACE.PTE), Dr. Mestyán Gyula (MELPAAP.PTE), Dr. Palkovics Tamás (PATHAAO.PTE), Dr. Schneider György (SCGQAAP.PTE), Dr. Szereday László (SZLPAAP.PTE), Dr. Tigyi Zoltán (TIZHAAE.PTE)
45
OMA017 M
OLECULARP
ATHOLOGYCourse director: DR.LÁSZLÓ PAJOR, professor
Department of Pathology 2 credit ▪ exam ▪ Basic module ▪ spring semester ▪ recommended semester: 2
Number of hours/semester: 28 lectures + 0 practices + 0 seminars = total of 28 hours Course headcount limitations (min-max.): min. 1 – max. 200
Prerequisites: none Topic
Molecular aberrations of tumours best characterised at the molecular level, will be presented at oncopathology lectures. The main emphasis will be on - apart from chronic myeloproliferative disorders, acute myeloid and lymphoid leukaemias, B- and T-cell malignant lymphomas - breast cancer, brain tumours (gliomas), HPV associated cervical carcinoma, colorectal and pulmonary epithelial tumours, and childhood soft tissue cancers. The lecture will also address existing and potential target therapies, their molecular basis, and system of criteria for predictive pathological diagnostics. The practical part of the course involves laboratory presentations, and experiments with active student participation. The experiments involve convential and molecular cytogenetics, automated interphase cytogenetic, multiparametric flow cytometry and cell sorting, DNA and RNA PCR, quantitative PCR, allele discrimination quantitative PCR and mutation analysis.
Conditions for acceptance of the semester
According to the Code of Studies and Examinations
Making up for missed classes
According to the Code of Studies and Examinations
Reading material
Margaret A. Knowles and Peter J. Selby: Introduction to the Cellular and Molecular Biology of Cancer, 4th edition, Oxford University
Press, USA 2005
Michael Khan, Stella Pelengaris: The Molecular Biology of Cancer. 1st edition, Wiley-Blackwell, 2006
Vincent T. DeVita, Theodore S Lawrence, Steven A. Rosenberg, Robert A Weinberg, Ronald A DePinho: DeVita, Hellman and Rosenberg’s Cancer: Principles & Practice of Oncology, 8th edition, Lippincott Williams & Wilkins, 2008
Lectures
1 1. Basic pathology. The consonance of morphological-, immunophenotypical and genotypical investigations in diagnostic pathology
Dr. Pajor László
2 1. Basic pathology. The consonance of morphological-, immunophenotypical and genotypical investigations in diagnostic pathology
Dr. Pajor László
3 2. Conventional cytogenetics and interphase fluorescence in situ hybridization (FISH) Dr. Kajtár Béla
4 2. Conventional cytogenetics and interphase fluorescence in situ hybridization (FISH) Dr. Kajtár Béla
5 3. Applications of PCR techniques in research and diagnostic pathology Dr. Kiss András Dr. Pajor László
6 3. Applications of PCR techniques in research and diagnostic pathology Dr. Kiss András Dr. Pajor László
7 4. Predictive molecular pathology of breast cancer and cervical carcinomas associated with HPV
Dr. Kálmán Endre
8 4. Predictive molecular pathology of breast cancer and cervical carcinomas associated with HPV
Dr. Kálmán Endre
9 5. Molecular pathology of mature B-, T- and NK-cell neoplasms and Hodgkin lymphoma Dr. Pajor László
10 5. Molecular pathology of mature B-, T- and NK-cell neoplasms and Hodgkin lymphoma Dr. Pajor László
11 6. Predictive molecular pathology of chronic myeloproliferative neoplasms and myelodysplastic syndromes Dr. Kajtár Béla
12 6. Predictive molecular pathology of chronic myeloproliferative neoplasms and myelodysplastic syndromes Dr. Kajtár Béla
13 7. Predictive molecular pathology of acute leukaemias.
Dr. Kereskai László
14 7. Predictive molecular pathology of acute leukaemias.
Dr. Kereskai László
15 8. Predictive molecular neuropathology. Dr. Molnár Péter Dr. Pajor László
16 8. Predictive molecular neuropathology. Dr. Molnár Péter Dr. Pajor László
17 9. Multicolour FISH based karyotyping Dr. Szuhai Károly Dr. Pajor László
18 9. Multicolour FISH based karyotyping Dr. Szuhai Károly Dr. Pajor László
19 10. Array-based karyotyping/aka digital karyotyping. Molecular karyotyping in practice Dr. Szuhai Károly Dr. Pajor László
20 10. Array-based karyotyping/aka digital karyotyping. Molecular karyotyping in practice Dr. Szuhai Károly Dr. Pajor László
21 11. Advanced imaging. FRET, FLIM, 3D and 4D imaging of live cells Dr. Szuhai Károly Dr. Pajor László
22 11. Advanced imaging. FRET, FLIM, 3D and 4D imaging of live cells Dr. Szuhai Károly Dr. Pajor László
23 12. One stop shop: next generation sequencing in research and pathology. „When chromosomes go mad: Understanding sarcomagenesis from the cytogenetic point of view”
Dr. Szuhai Károly Dr. Pajor László
24 12. One stop shop: next generation sequencing in research and pathology. „When chromosomes go mad: Understanding sarcomagenesis from the cytogenetic point of view”
Dr. Szuhai Károly Dr. Pajor László
25 13. Molecular pathology of lung cancer and colorectal carcinoma Dr. Kajtár Béla
Dr. Pajor László
26 13. Molecular pathology of lung cancer and colorectal carcinoma Dr. Kajtár Béla Dr. Pajor László 27 Exam Dr. Pajor László 28 Exam Dr. Pajor László Practices Seminars Exam topics/questions Participants
Dr. Kajtár Béla (KABFAAO.PTE), Dr. Kálmán Endre (KAEMAAO.PTE), Dr. Kereskai László (KELMAAO.PTE), Dr. Pajor László (PALGAAO.PTE), Dr. Tornóczki Tamás (TOTMABO.PTE)
47
OMA018 D
EVELOPMENTALB
IOLOGYCourse director: DR.PÉTER BALOGH, associate professor
Department of Immunology and Biotechnology 2 credit ▪ exam ▪ Basic module ▪ spring semester ▪ recommended semester: 2
Number of hours/semester: 28 lectures + 0 practices + 0 seminars = total of 28 hours Course headcount limitations (min-max.): min. 5 – max. 30
Prerequisites: none Topic
This course covers the molecular and cellular bases of development in a variety of experimental organisms with special emphasis on mammalian and human models. With the observation of differential gene-activity and geneexpression patterns it explains the development of various tissues and organs. The course considers the possible pharmacological and pharmacotherapical applications in the biological development.
Conditions for acceptance of the semester
Regular attendance, mid-term test writing.
Making up for missed classes
None
Reading material
1. Scott F. Gilbert: Developmental Biology, Sinauer Associates, 2006
2. Lewis Wolpert, Jim Smith, Tom Jessell, and Peter Lawrence: Principles of Development, 3rd edition, Oxford University Press
3. Jonathan M. W. Slack: Essential Developmental Biology, Blackwell, 2006 4. B.K. Hall: Evolutionary Developmental Biology, 2nd edition, Kluwer
Lectures
1 Developmental biology: The anatomical tradition Dr. Balogh Péter
2 Comparative and Evolutionary Embryology Dr. Balogh Péter
3 Medical Embryology and Teratology Dr. Balogh Péter
4 Genes and development: Techniques and ethical issues. Dr. Balogh Péter
5 The genetic core of development: Differential gene expression and RNA Localization Techniques Dr. Engelmann Péter András
6 Cell-cell communication in development Dr. Balogh Péter
7 Germ line and gametogenesis Dr. Engelmann Péter András
8 Early embryonic development: Structure of the Gametes, Recognition of Egg and Sperm Dr. Balogh Péter
9 Gamete Fusion and synchronization Dr. Balogh Péter
10 Early Mammalian Development Dr. Balogh Péter
11 Formation and differentiation of the Neural Tube Dr. Engelmann Péter András
12 Tissue Architecture of the Central Nervous System and Neuronal Types Dr. Engelmann Péter András
13 Development of the Vertebrate Eye Dr. Balogh Péter
14 The Epidermis and the Origin of Cutaneous Structures Dr. Balogh Péter
15 Neural crest cells and axonal specificity Dr. Engelmann Péter András
16 Paraxial and intermediate mesoderm Dr. Balogh Péter
17 Development of haematopoiesis Dr. Balogh Péter
18 Development of primary and secondary lymphoid organs Dr. Balogh Péter
19 The Development of Muscle Dr. Balogh Péter
20 The Development of Bones Dr. Balogh Péter
21 Lateral plate mesoderm and endoderm Dr. Balogh Péter
22 Limb development: generation of three axes and their coordination Dr. Balogh Péter
23 Cell Death and the Formation of Digits and Joints Dr. Balogh Péter
24 Sex determination in Mammals: Chromosomal and environmental Dr. Balogh Péter
25 Metamorphosis and regeneration Dr. Balogh Péter
26 Environmental regulation of animal development Dr. Balogh Péter
27 Evolution and development Dr. Balogh Péter
28 Therapeutic developmental biology Dr. Balogh Péter Practices Seminars Exam topics/questions None Participants
49