Course director: DR.JUDIT PONGRÁCZ, professor
Department of Pharmaceutical Biotechnology 1 credit ▪ exam ▪ Elective courses (Med. Biot.) module ▪ autumn semester ▪ recommended semester: 3
Number of hours/semester: 14 lectures + 0 practices + 0 seminars = total of 14 hours Course headcount limitations (min-max.): min. 5 – max. 150
Prerequisites: none Topic
Gene therapy, in vivo gene therapy
Conditions for acceptance of the semester
According to the Code of Studies and Examinations
Making up for missed classes
None Reading material www.medbiotech.com Lectures 1 Functional genomics I. Dr. Pongrácz Judit 2 Functional genomics II.
Dr. Pongrácz Judit
3 Recombinant protein expression Dr. Pongrácz Judit
4 Gene therapy, in vivo gene therapy Dr. Pongrácz Judit
5 Ex vivo gene therapy, Therapies based on protein replacement I. Dr. Pongrácz Judit
6 Therapies based on protein replacement II. Dr. Pongrácz Judit
7 Recombinant antibodies and phage phage display technique. Dr. Pongrácz Judit
8 Anti-cytokine therapy (Sepsis) Dr. Pongrácz Judit
9 Transgene technology and mouse models in modern biological research. Dr. Pongrácz Judit
10 Embryonic and adult stem cells for regenerative medicine I. Dr. Pongrácz Judit
11 Embryonic and adult stem cells for regenerative medicine II. Dr. Pongrácz Judit
12 Cell cycle and cancer therapy, p53 I. Dr. Pongrácz Judit
13 Cell cycle and cancer therapy, p53 II. Dr. Pongrácz Judit
14 Gene silencing technologies Dr. Pongrácz Judit Practices Seminars Exam topics/questions www.medbiotech.com Participants
105
OMD041 P
ROTEINB
IOTECHNOLOGYCourse director: DR.JUDIT PONGRÁCZ, professor
Department of Pharmaceutical Biotechnology 1 credit ▪ exam ▪ Elective courses (Med. Biot.) module ▪ autumn semester ▪ recommended semester: 3
Number of hours/semester: 14 lectures + 0 practices + 0 seminars = total of 14 hours Course headcount limitations (min-max.): min. 5 – max. 150
Prerequisites: none Topic
Biochemical properties of proteins. Protein synthesis. Review of the differences between procaryotic and eucaryotic protein synthesis.
Conditions for acceptance of the semester
According to the Code of Studies and Examinations
Making up for missed classes
None
Reading material
www.medbiotech.com
Lectures
1 Biochemical properties of proteins. Protein synthesis. Review of the differences between procaryotic and eucaryotic protein synthesis.
Dr. Pongrácz Judit
2 Protein folding, the formation of 3D structure. Chaperons. Folding problems and folding diseases. Dr. Pongrácz Judit
3 Protein sorting and targeting. Dr. Pongrácz Judit
4 Study of protein structures: X-ray crystallography, NMR, mass spectrometry. Dr. Pongrácz Judit
5 Protein purification (chromatography, electrophoresis) and analysis (SDS-PAGE, 2 dimensional electrophoresis, mass spectrometry).
Dr. Pongrácz Judit
6 Posttranslational modification of proteins and their analysis using proteomics methods. Dr. Pongrácz Judit
7 Protein-protein interactions and their analysis: yeast two hybrid system, mass spectrometry, Biacore and other techniques. Dr. Pongrácz Judit
8 Heterologue expression I: Homologue and heterologue expression, biopharming; expression systems, criteria to the selection of expression platforms, heterologue expression in Escherichia coli, formation of inclusion body, periplasmic secretion, formation of disulfide bonds.
Dr. Pongrácz Judit
9 Heterologue expression II: The disadvantages of the Escherichia coli system, development of new expression platforms; protein expression in gram-positive bacteria; the Staphylococcus carnosus expression system; Protein expression in yeasts; the Saccharomyces cerevisiae and the Pichia pastoris expression platforms.
Dr. Pongrácz Judit
10 Heterologue expression III: The disadvantages of the microbial protein expression; heterologue expression in plant cell cultures, transformation with Agrobacteria, expression in mammalian cell, functionalized cells; heterologue expression in insect cell lines, the baculovirus-insect cell expression system.
Dr. Pongrácz Judit
11 Definition of protein engineering; de novo design, artificial growing factors; rational design, site-directed mutagenesis; directed evolution, random mutagenesis, DNA shuffling, exon shuffling.
Dr. Pongrácz Judit
12 Production of human therapeutic proteins. Biopharmaceuticals, production of human therapeutic proteins; production of insulin, protein engineering of insulin; development of recombinant Hepatitis B vaccine; production of monoclonal antibodies; problems with the storage, in vivo stability and administration of therapeutic proteins.
13 Production of human therapeutic enzymes: Enzymes in human therapy; production, and human therapeutically application of deoxyribonuclease I, -glucocerebrosidase, urate oxidase and L-asparaginase, mechanism of action.
Dr. Pongrácz Judit
14 Production of diagnostic enzymes. Importance of enzymes in diagnosis, production and application of glucose oxidase, galactose oxidase, cholesterol oxidase and horseradish peroxidase; protein engineering of enzymes. The application of therapeutic proteins. Possible administration, perspectives and future possibilities. The national and international requirements for protein therapeutic products.
Dr. Pongrácz Judit Practices Seminars Exam topics/questions www.medbiotech.com Participants
107
OMD049 B
ASICS OFP
HYTOTHERAPYCourse director: DR.JUDIT PONGRÁCZ, professor
Department of Pharmaceutical Biotechnology 1 credit ▪ exam ▪ Elective courses (Med. Biot.) module ▪ autumn semester ▪ recommended semester: 3
Number of hours/semester: 14 lectures + 0 practices + 0 seminars = total of 14 hours Course headcount limitations (min-max.): min. 1 – max. 30
Prerequisites: none Topic
The basic knowledge about phytotherapy, the student’s awareness of the phytotherapy or their activities and tasks of the races.
Conditions for acceptance of the semester
Maximum of 25 % absence allowed
Making up for missed classes
None
Reading material
www.medbiotech.com
Lectures
1 The history of natural medicine, philosophical foundations. The phytotherapy as a natural healing method.
Kovácsné Kósa Judit
2 Historical overview of the development of phytotherapy. The location of the phytotherapy within classical methods of Natural Medicine.
Kovácsné Kósa Judit
3 The regulation of Natural Medicine. Basic definitions in the phytotherapy.
Kovácsné Kósa Judit
4 Herbs I.: Rules of collection and storage; Typical quality defects.
Kovácsné Kósa Judit
5 Herbs II.: Drug Testing; Protected and endangered species.
Kovácsné Kósa Judit
6 Healthy food plants, herbs, mushrooms and juices.
Kovácsné Kósa Judit
7 Classification by substances I.
Kovácsné Kósa Judit
8 Classification by substances II.
Kovácsné Kósa Judit
9 Classification by substances III.
Kovácsné Kósa Judit
10 Classification by substances IV.
Kovácsné Kósa Judit
11 Causal and symptomatic treatment in Phytotherapy.
Kovácsné Kósa Judit
12 Making prescription. Herbs and their underactions.
Kovácsné Kósa Judit
13 Parts of the treatment plan and its practical application. The treatment from ethical perspective.
Kovácsné Kósa Judit
14 The priority areas of Phytotherapy: Apitherapy, Aromatherapy and the Bach Flower Therapy.
Kovácsné Kósa Judit
Practices Seminars
Exam topics/questions
www.medbiotech.com