Course director: DR.PÉTER BALOGH, associate professor
Department of Immunology and Biotechnology 4 credit ▪ exam + progress grade ▪ Specialty in Cell- and Tissue-engineering module ▪ autumn semester ▪ recommended semester: 3
Number of hours/semester: 28 lectures + 28 practices + 0 seminars = total of 56 hours Course headcount limitations (min-max.): min. 1 – max. 30
Prerequisites: none Topic
The course provides an overview on the molecular regulation of tissue-specific regulatory mechanisms during mammalian organogenesis.
Conditions for acceptance of the semester
Maximum of 15 % absence allowed
Making up for missed classes
None
Reading material
MEDLINE database
Lectures
1 Introduction, basic concepts I. Dr. Balogh Péter
2 Introduction, basic concepts II. Dr. Balogh Péter
3 Developmental biology: Basic concepts, mechanisms and tools I. Dr. Balogh Péter
4 Developmental biology: Basic concepts, mechanisms and tools II. Dr. Balogh Péter
5 General morphogenic factors and their characteristics: Shh, BMP, Wnt, Notch I. Dr. Balogh Péter
6 General morphogenic factors and their characteristics: Shh, BMP, Wnt, Notch II. Dr. Balogh Péter
7 Epigenetic factors in development I. Dr. Najbauer József
8 Epigenetic factors in development II. Dr. Najbauer József
9 Development of blood cells I. Dr. Balogh Péter
10 Development of blood cells II. Dr. Balogh Péter
11 Leukemias and lymphoid malignancies I. Dr. Balogh Péter
12 Leukemias and lymphoid malignancies II. Dr. Balogh Péter
13 Molecular regulation of skeletal muscle development I. Dr. Balogh Péter
14 Molecular regulation of skeletal muscle development II. Dr. Balogh Péter
15 Molecular regulation of liver development I. Dr. Engelmann Péter András
16 Molecular regulation of liver development II. Dr. Engelmann Péter András
79 17 Molecular regulation of pancreas development I.
Dr. Engelmann Péter András
18 Molecular regulation of pancreas development II. Dr. Engelmann Péter András
19 Molecular development of neural circuits: synapsins and netrins I. Dr. Engelmann Péter András
20 Molecular development of neural circuits: synapsins and netrins II. Dr. Engelmann Péter András
21 Molecular development of heart and vasculature I. Dr. Najbauer József
22 Molecular development of heart and vasculature II. Dr. Najbauer József
23 Stepwise development of ureteric bud and metanephros I. Dr. Engelmann Péter András
24 Stepwise development of ureteric bud and metanephros II. Dr. Engelmann Péter András
25 Skin development
Dr. Najbauer József
26 Molecular mechanisms of malignancies I. Dr. Najbauer József
27 Molecular mechanisms of malignancies II. Dr. Najbauer József
28 Consultation. Dr. Engelmann Péter András
Practices
1 Introduction: course aims, lab rules I. 2 Introduction: course aims, lab rules II. 3 AP staining of trophoblastic cells I. 4 AP staining of trophoblastic cells II. 5 Immunofluorescence and flow cytometry I. 6 Immunofluorescence and flow cytometry II.
7 Isolation of bone marrow cells for MSC initiation and bone marrow chimerism I. 8 Isolation of bone marrow cells for MSC initiation and bone marrow chimerism II. 9 Initiation of invertebrate regeneration model I.
10 Initiation of invertebrate regeneration model II. 11 Isolation of mouse zygotes I.
12 Isolation of mouse zygotes II.
13 Isolation of embryonic tissues for in vitro propagation I. 14 Isolation of embryonic tissues for in vitro propagation II. 15 Immunofluorescence of select embryonic tissue sections I. 16 Immunofluorescence of select embryonic tissue sections II. 17 Flow cytometry of embryonic cultures I.
18 Flow cytometry of embryonic cultures II. 19 Initiation of cellular co-culture I. 20 Initiation of cellular co-culture II.
21 Fibroblast-endothelial cell marking and co-culture I. 22 Fibroblast-endothelial cell marking and co-culture II. 23 Flow cytometric analysis of hemopoietic chimeras I. 24 Flow cytometric analysis of hemopoietic chimeras II. 25 Thymus sorting I.
26 Thymus sorting II.
27 Consultation I.
Seminars
Exam topics/questions
None
Participants
81
OMC002 T
RANSDIFFERENTIATIONCourse director: DR.BALOGH PÉTER, egyetemi docens
Department of Immunology and Biotechnology 4 credit ▪ exam + progress grade ▪ Specialty in Cell- and Tissue-engineering module ▪ autumn semester ▪ recommended semester: 3
Number of hours/semester: 14 lectures + 42 practices + 0 seminars = total of 56 hours Course headcount limitations (min-max.): min. 5 – max. 30
Prerequisites: none Topic
The aim of the course is to present the techniques of tissue transformation (e.g. establishment of immortalized cell lines) and practical uses of the techniques. The course will cover possibilities of genetic reprogramming in both differentiated cells and stem cells. The course will cover in detail the design and cloning of tissue specific transcription factors and promoters, and will present various methods for incorporating these elements into primary cells.
Conditions for acceptance of the semester
Regular attendance and mid-term test writing
Making up for missed classes
None Reading material None Lectures 1 Introduction Dr. Balogh Péter 2 Types of stem cells
Dr. Balogh Péter
3 Animal models of regeneration Dr. Engelmann Péter András
4 Ethics of stem cell research and therapy Dr. Balogh Péter
5 Genetic and epigenetic factors in stem cell regulation Dr. Najbauer József
6 Methods in stem cell research Dr. Engelmann Péter András 7 Stem cells and hematopoiesis
Dr. Balogh Péter
8 Muscle regeneration and stem cells Dr. Balogh Péter
9 Liver regeneration and stem cells Dr. Engelmann Péter András 10 Pancreas regeneration and stem cells
Dr. Balogh Péter
11 Cardiovascular regeneration and stem cells Dr. Najbauer József
12 Neural regeneration and stem cells Dr. Najbauer József
13 Kidney regeneration Dr. Engelmann Péter András 14 Cancer stem cells
Practices 1 .. 2 .. 3 .. 4 .. 5 .. 6 .. 7 .. 8 .. 9 .. 10 .. 11 .. 12 .. 13 .. 14 .. 15 .. 16 .. 17 .. 18 .. 19 .. 20 .. 21 .. 22 .. 23 .. 24 .. 25 .. 26 .. 27 .. 28 .. 29 .. 30 .. 31 .. 32 .. 33 .. 34 .. 35 .. 36 .. 37 .. 38 .. 39 .. 40 .. 41 .. 42 .. Seminars Exam topics/questions None Participants
83
OMC003 B
IOLOGICALT
HERAPIESCourse director: DR.PÉTER NÉMETH, professor
Department of Immunology and Biotechnology 4 credit ▪ exam + progress grade ▪ Specialty in Cell- and Tissue-engineering module ▪ autumn semester ▪ recommended semester: 3
Number of hours/semester: 28 lectures + 28 practices + 0 seminars = total of 56 hours Course headcount limitations (min-max.): min. 3 – max. 200
Prerequisites: none Topic
The aim of the course is to present the molecular level pathomechanism of diseases targeted by biological therapies, and to cover the targets of protein based medicinal products, and the methods used to produce these medicinal products. The course discusses in detail the medicinal products applied during substitution, immunosuppressive, anti-viral, anti-tumor, anti-inflammatory therapies, and during therapy of autoimmune diseases.
Conditions for acceptance of the semester
No more absence as 3.
Making up for missed classes
No
Reading material
1. Bruce Kirkham, Arthur Kavanaugh, Scott Eric Plevy, and Jonathan Barker: The Handbook of Biological Therapy, Oxford University Press, 2009 ISBN-10: 0199208166
2. Pongracz J, Keen M (eds.): Medical Biotechnology, Elsevier, 2009
3. Bela Bodey, Stuart E. Siegel, and Hans E. Kaiser: Molecular Markers of Brain Tumor Cells: Implications for Diagnosis, Prognosis and Anti-Neoplastic Biological Therapy, Kluwer, 2004
Lectures
1 Introduction to biological therapies Dr. Németh Péter
2 Targeting immune reactions against well conserved antigens Dr. Németh Péter
3 Generation of conserved antigens Dr. Németh Péter
4 Immunosuppression on autoimmune diseases Dr. Pongrácz Judit
5 Methods of immunosuppression Dr. Pongrácz Judit
6 Immunosuppressive therapy as a determinant of transplantation outcomes Dr. Pongrácz Judit
7 Biotherapies of Systemic autoimmune diseases Dr. Pongrácz Judit
8 Application of immunological tolerance Dr. Pongrácz Judit
9 Non-specific and antigens specific immunosuppression for protection of grafted tissues Dr. Pongrácz Judit
10 Using antibodies in pathological immune regulation Dr. Pongrácz Judit
11 Biotherapies of allergic diseases Dr. Pongrácz Judit
12 Biotechnological aspects of organ transplantation Dr. Pongrácz Judit
13 Biotherapies of hypersensitive immunereactions Dr. Pongrácz Judit
14 Clinical aspects of autoimmune diseases Dr. Czirják László István
15 Clinical aspects of autoimmune diseases 2 Dr. Czirják László István
16 Biotherapies of solid tumours Dr. Pongrácz Judit
17 New generation of biotherapies in malignant diseases Dr. Pongrácz Judit
18 Targeted therapeutical design Dr. Pongrácz Judit
19 Delivery vectors of biotherapeutics Dr. Pongrácz Judit
20 Dangers of recombinant viral vectors as delivery agents of biotherapeutics Dr. Pongrácz Judit
21 Nanotherapeutics Dr. Pongrácz Judit
22 Nanodesign in biological therapies Dr. Pongrácz Judit
23 Monoclonal antibodies as therapeutic agents Dr. Pongrácz Judit
24 Monoclonal antibodies as delivery vectors in therapy Dr. Pongrácz Judit
25 Biotherapies for inherited genetic diseases Dr. Pongrácz Judit
26 Identification of therapeutic targets in genetical diseases Dr. Pongrácz Judit
27 Humanization of antibodies used in therapy Dr. Németh Péter
28 The future of biological therapies Dr. Németh Péter
Practices
1 Immunological background of immunoglobulin production 2 Immunisation and vaccination.
3 Polyclonal and monoclonal antibody production. 4 Practical approaches of immunisation protocolls. 5 Polyclonal antibody production for research use.
6 Methodical background of hybridoma technique and monoclonal antibody development. 7 Monoclonal antibody development for research use.
8 Requirement for therapeutic monoclonal antibody development. 9 Humanized and chimeric antibodies for therapeutic use. 10 Genetically engineered monoclonal antibodies. 11 Chimeric antibody technology.
12 Humanization technique in therapeutic monoclonal antibody production. 13 Therapeutoc relevances of genetically engineered monoclonal antibodies. 14 Hybridoma fermentation for laboratory use.
15 Problems of large scale monoclonal antibody production.
16 Fermentation technologies applied in therapeutic monoclonal antibody production. 17 Pharmacological aspects of therapeutic monoclonal antibody production.
18 General role of cytokines in physiologic immunoregulation. 19 Role of cytokines in pathologic reactions.
20 Basic aspects of cytokine therapy. 21 Recombinant cytokines and interleukines. 22 General aspects of interferon therapy.
23 Purification of human interferons from peripheral blood leukocytes. 24 Recombinant human interferons.
85 25 Overview of stem cell techniques.
26 General aspects of human stem cell therapy. 27 Quality assurance of human biotherapies. 28 Ethical aspects of biotherapies.
Seminars
Exam topics/questions
Written exam. The questions prepared according to lectures and practices. Details see in www.medbiotech.com
Participants