16S rDNA gene
Identification of the first Transgenic Aquatic Animal in Iran by PCR-Based Method and Protein Analysis
... Mitochondrial 16S rDNA was selected and amplified. The validation of 16S rDNA gene sequences data detected three haplotypes deposited in the ...a gene correspondence red color of ...
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Genetic diversity of Bacillus sp producers of amylase isolated from the soil.
... reaction volume of 50 µL. The conditions were as follows: an initial cycle of denaturation at 94°C for 5 min followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 57°C for 30 s, extension at 72°C for 45 s, ...
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16S rDNA Identification and molecular characterization of xanthomonadaceae nml 03 0222 bacterial isolates from agricultural soil of jharkhand
... The 16S rDNA molecular gene sequencing method is one of the advanced and recently used methods for the identification of ...Furthermore, 16S rDNA gene sequencing approach also ...
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Characterization of bacterial biota in the distal esophagus of Japanese patients with reflux esophagitis and Barrett’s esophagus
... Phylum-level analysis of our present samples revealed that the bacterial communities differed among groups. First, each group had a different number of phyla: populations could be classified into four phyla in patients ...
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Identification of Lactobacilli and Bifidobacteria
... of 16S rDNA gene sequences from lactobacilli shows that the V1, V2 and V3 regions contain the species-specific ...the gene (less than 750 bp) may be sufficient, but whole gene ...
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Two different 16S rRNA genes in a mycobacterial strain
... the gene coding for 16S rRNA (16S rDNA) is a well-established method used to identify bacteria, particularly ...one 16S rDNA is present on one mycobacterial genome, their ...
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Metagenomic rDNA Profiles of the Oral Microbiome in Healthy Subjects Are Distinctive Compared to That in Smokers and Disease Subjects Despite High Variations between Subjects
... the 16S rDNA gene in me- tagenomic samples, this study evaluated the stability of the oral microbiome and contrasted the PCR-DGGE profiles of subjects belonging to three groups —healthy, smokers and ...
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Bacterial Identification for Publication: When Is Enough Enough?
... (16S) rDNA genes had be- come commonplace, considered a standard tool of microbial taxonomists not only for elucidating phylogenetic relatedness but also as a means of bacterial identification (15, ...of ...
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ISOLATION AND MOLECULAR IDENTIFICATION OF AQUATIC BACTERIA FROM TIGRIS RIVER WITHIN WASIT PROVINCE
... of 16S rDNA gene was 99% identical for Bacillus subtilis, Escherichia coli, Bacillus ...of 16S rDNA gene was 100% identical for Aeromonas caviae as shown in the table ...
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Molecular Study of a New Disease of Peach in Iran Associated with a Phytoplasma
... Phytoplasmal rDNA was amplified in either single or nested PCR ...entire 16S rDNA gene and all of the 16S-23S spacer regions; R16F2 and R16R2 [12] for ...
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Applicability of PCR DGGE and 16S rDNA Sequencing for Microbiological Analysis of Otitis Media with Effusion
... Following DGGE analysis of PCR amplicons revealed that each MEEs consisted of multiple 16S rDNA gene sequences, each assumed to represent a unique bacterial DNA (Figure 1). The number of clearly ...
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High Throughput DNA-DNA Microarray Chip Strategy for Detection and Identification of Enteropathogenic bacteria
... partial 16S rDNA gene fragment (173bp) as target for positive internal ...coli 16S rRNA gene) we can use the same primer sets as one of the components of multiplex PCR wherever ...
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Broad range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian patients with reactive and undifferentiated arthritis
... Bacterial 16S rDNA fragments were amplified from SF extracted DNA by broad-range PCR amplification using uni- versal bacterial 16S rRNA gene-specific oligonucleotide prim- ers Bac08 and ...
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Diagnostics of Neisseriaceae andMoraxellaceae by Ribosomal DNA Sequencing: Ribosomal Differentiation of Medical Microorganisms
... inadequate. 16S or 23S ribosomal DNA (rDNA) bacterial sequencing will most often result in accurate speciation of ...hypervariable rDNA stretch, flanked by strongly conserved regions, which is ...
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Obersteiner, Andrea (2017): Pollen associated microbiome and its relationship to pollution and allergens. Dissertation, LMU München: Fakultät für Biologie
... Beginning with cultivation-dependent approaches, the colonization of allergenic pollen was demonstrated by identification of bacterial and fungal species isolated from birch and timothy grass pollen. Next, ...
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Terrestrial actinomycetes from diverse locations of Uttarakhnad, India: Isolation and screening for their antibacterial activity.
... The 16S rDNA sequences (900 bp) of the strains DV1S and GR9a-5 were determined and submitted to GenBank under the accession number HM991289, ...shared 16S rRNA gene sequence similarity of ...
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A system to simultaneously detect tick borne pathogens based on the variability of the 16S ribosomal genes
... It should be noted that many ticks harbor non-pathogenic bacteria, including Coxiella-like [53-56], Rickettsia-like [57-59] and Francisella-like [60,61] endosymbionts, which are in a mutualistic relationship with the ...
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‘Candidatus Aquirickettsiella gammari’ (Gammaproteobacteria: Legionellales: Coxiellaceae): A bacterial pathogen of the freshwater crustacean Gammarus fossarum (Malacostraca: Amphipoda)
... Raw sequences were quality-trimmed using Trimmomatic v0.32 (Bolger et al., 2014) with the following parameters: NexteraPE- PE.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36. Paired-end reads were merged ...
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Comparing 16S rDNA amplicon sequencing and hybridization capture for pea aphid microbiota diversity analysis
... 16S rRNA gene capture was carried out as described by Denonfoux et al. [7]. Libraries were prepared using Roche GS FLX Rapid Library Preparation Kit (Roche Applied Science) according to the manufacturer’s ...
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Isolation and Characterization of a Black PigmentedCorynebacterium sp from a Woman with Spontaneous Abortion
... Phylogenetic analysis. The rDNA sequence of Corynebacterium sp. strain CN-1 was aligned with a database of archaeal, bacterial, and eucaryal small- subunit rRNA sequences (ca. 8,000 sequences in total) using the ...
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