A) Fresh and fixed specimens
High resolution diffusion weighted MR of fresh and fixed cat spinal cords: evaluation of diffusion coefficients and anisotropy
... cord specimens. METHODS: Four cat cord specimens were imaged using a diffusion-weighted spin-echo ...the fixed specimens, calculated ADC images confirm that diffusion anisotropy is ...of ...
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Biobanking of fresh-frozen endoscopic biopsy specimens from esophageal adenocarcinoma
... For RNA sequencing, protocols allowing the input of minute quantities of degraded RNA have been devel- oped, but sequencing results are still better with pro- tocols requiring intact RNA. 18 Because archival ...
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Quantitative analysis of the terminal branches of facial nerve in fresh frozen head and neck specimens
... formalin fixed cadavers, it is very difficult to find ...branches; fresh or fresh frozen human samples are ...in fresh frozen head and neck ...
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Quality control material for the detection of somatic mutations in fixed clinical specimens by next-generation sequencing
... four fresh-frozen cell line pellets was further diluted in DNA isolated from a de-identified normal human ...for fresh-frozen samples, when barcoding up to 7 ...
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Analysis of microRNA from archived formalin-fixed paraffin-embedded specimens of amyotrophic lateral sclerosis
... that miRNAs may be good biomarkers for cancer diag- nosis and prognosis [5]. The role of miRNAs has also been studied in neurodegenerative conditions such as Alzheimer?s disease [6-22], Parkinson? s disease [19,23-27], ...
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Quality control material for the detection of somatic mutations in fixed clinical specimens by next-generation sequencing
... All tissue H&E stained slides were reviewed by a patholo- gist who assessed percent tumor content and delineated the tumor area for manual microdissection, if needed. Ten-micron unstained FFPE slides were then ...
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Ultra percutaneous dilation tracheotomy vs mini open tracheotomy A comparison of tracheal damage in fresh cadaver specimens
... An open mini tracheostomy was performed on seven fixed cadaver and ten fresh cadaver. A 1 cm vertical inci- sion was used and positioned 1 cm below the cricoid car- tilage (Figure 1). The strap muscles were ...
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Detection and Verification of Mycobacterium avium subsp paratuberculosis in Fresh Ileocolonic Mucosal Biopsy Specimens from Individuals with and without Crohn's Disease
... with fixed gut tissue from 27 of 33 (82%) CD patients in Italy ...biopsy specimens from through- out the gut from about half of the PCR-positive patients sug- gests that ...
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Measurement of breast-tissue x-ray attenuation by spectral imaging: fresh and fixed normal and malignant tissue.
... tumour specimens, 7 fibro-glandular tissue specimens, and 15 adipose tissue specimens were ...between fresh tumour tissue and cyst fluid was smaller than has previously been measured for ...
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Simple differential detection of Entamoeba histolytica and Entamoeba dispar in fresh stool specimens by sodium acetate acetic acid formalin concentration and PCR
... stool specimens, which all contained ...stool specimens, all 18 samples examined were positive by PCR, whereas by using samples stored in SAF for more than 2 days, only 7 of 19 samples ...stool ...
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Microarray analysis of RNA extracted from formalin-fixed, paraffin-embedded and matched fresh-frozen ovarian adenocarcinomas
... FFPE specimens is often better preserved than in matched fresh-frozen (FF) counterparts, the quality of nucleic acids in FFPE samples is far from optimal due to chemical crosslinking and nucleic acid ...
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Success of tumorsphere isolation from WHO grade IV gliomas does not correlate with the weight of fresh tumor specimens: an immunohistochemical characterization of tumorsphere differentiation
... Immunocytochemical staining For investigation of surface and intracellular antigen expression profiles, TSs were transferred to cover slides, fixed with 2 % paraformaldehyde for 7 min, and then treated with a 3:1 ...
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A Comparison of RNA-Seq Results from Paired Formalin-Fixed Paraffin-Embedded and Fresh-Frozen Glioblastoma Tissue Samples
... use. Fresh frozen (FF) tis- sue specimens of GBM are difficult to obtain since tumor tissue obtained at surgery is often scarce and necrotic and diagnosis is prioritized over ...GBM specimens ...
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Comparison of quantitative real time PCR with Sequencing and ribosomal RNA FISH for the identification of fungi in Formalin fixed, paraffin embedded tissue specimens
... tissue specimens Paraffin was removed from tissue sections by dipping the slides into 99% octane for 15 seconds at room tempera- ...a fresh solution of 100 mM Tris hydrochloride (pH = 8) and 50 mM sodium ...
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Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in Formalin fixed, paraffin-embedded tissue specimens
... tissue specimens Paraffin was removed from tissue sections by dipping the slides into 99% octane for 15 seconds at room tempera- ...a fresh solution of 100 mM Tris hydrochloride (pH = 8) and 50 mM sodium ...
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Vital immunohistochemical staining for a novel method of diagnosing micro-cancer -examination of immuno-histochemical staining of non-fixed fresh tissue-
... non-fixed fresh tissue specimens of these organs were exposed to ...antibodies. Specimens of esophageal mucosa pretreated with a mixture of pronase and NaHCO 3 showed positive responses to the ...
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Techniques for examining Pneumocystis carinii in fresh specimens
... Pneumocystis carinii was examined in fresh preparations of infected rat lung homogenates and tissue culture supernatants by a variety of light microscope techniques, vital dyes, and hist[r] ...
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Preserved and fixed animal and human specimens
... human specimens that are preserved or fixed, for end uses other than fertiliser, animal feed or growing ...include specimens such as phytoplankton, zooplankton, preserved or fixed insects and ...
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4Pi-Confocal Imaging in Fixed Biological Specimens
... So far, two concepts promise fundamentally increased axial resolution: standing-wave microscopy (SWM) and 4Pi mi- croscopy. The sharp interference maxima of the SWM pro- vide 50 –120-nm fine layers of excitatory light ...
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DNA/RNA Degradation Rate in Long Term Fixed Museum Specimens
... of specimens from museum jars from the Pathology Department at the Indiana University School of Medicine, the sequential and chronological degradation of DNA and RNA has been ...gross specimens from 1920 to ...
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