Active-site residues
Identification of the active-site residues of the L proteinase of foot-and-mouth disease virus.
... His residues, His-110, partially reduced cleav- age of p220, indicating that a change at this position could alter the activity of the ...36 residues (amino acids 56 to 91) from papain (note that Lb is ...
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Importance of Neuraminidase Active-Site Residues to the Neuraminidase Inhibitor Resistance of Influenza Viruses
... NA residues will also help to refine the design of existing ...NA residues may decrease NA enzymatic activity (10, 22, 23, ...design, site-directed mu- tagenesis has been used to investigate the ...
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Identification of active-site residues in protease 3C of hepatitis A virus by site-directed mutagenesis.
... proteolytic cascade was also active on P3 polyprotein when synthesized by in vitro translation. The relevance of these ob- servations in heterologous systems was confirmed by identifi- cation of peptide species ...
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IN SILICO DOCKING STUDIES OF PHYTO LIGANDS AGAINST E COLI PBP3: APPROACH TOWARDS NOVEL ANTIBACTERIAL THERAPEUTIC AGENT
... Due to emergence of multi drug resistance in bacteria, there is an urgent need to search for new antibacterial agents. Medicinal plants reveal important pharmacological activities that can be applied to developing novel ...
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Variable Structural Networks at the Active Site of the SARS-CoV and SARS-CoV2 Main Proteases
... variable residues, and their X-ray structures appear ...variable residues affect the intra-molecular interactions between key residues in the CoV2 M pro ...divergent residues in CoV2 M pro ...
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IN-SILICO ANALYSIS OF ANDROGRAPHOLIDE AGAINST CANCER
... the active site residues Thr51, Asn20 and ...the residues Thr51, Asn20, Tyr330 Arg93, Trp30, Arg93, Arg190 and Lys192 at the active site of ...
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Identification of Active-Site Amino Acid Residues in the Chiba Virus 3C-Like Protease
... identify active-site amino acid residues in this protease, 37 charged amino acid residues and a putative nucleophile, Cys139, within the GDCG sequence were individually replaced with Ala in ...
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Crystal Structures of Two Coronavirus ADP-Ribose-1″-Monophosphatases and Their Complexes with ADP-Ribose: a Systematic Structural Analysis of the Viral ADRP Domain
... The active-site residues responsible for mediating ADRP activity were found to be highly conserved in terms of both sequence alignment and structural superposition, whereas the substrate binding ...
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Catalytic Mechanism and Mode of Action of the Periplasmic Alginate Epimerase AlgG
... of Active Site Residues of Periplasmic and Extracellular Alginate Epimerases Does Not Extend to the Poly- mer Binding Site—Although both periplasmic AlgG and the extracellular epimerase AlgE4 ...
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The 5' end of the equine arteritis virus replicase gene encodes a papainlike cysteine protease.
... To prove that the EAV PCP domain was responsible for the observed proteolytic processing of the ORFla protein and to identify possible active site residues, single amino acid substitutio[r] ...
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Page_unc_0153D_15827.pdf
... Considering the roles of RRs and Hpts in phosphotransfer reactions, the variation in relative sensitivities between CheY variants reported here may reflect some aspect of RR/Hpt function in phosphorelays. From the ...
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Journal of Applied Pharmaceutical Science
... key active site residues (Tyr48, His110 and ...other active site residues, in particular ...the active site of aldose reductase and interact with the ...
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Crystal Structures of Enterovirus 71 3C Protease Complexed with Rupintrivir Reveal the Roles of Catalytically Important Residues
... Invariant residues are white with a red background; conserved residues are shown in red ...The residues involved in the interactions with rupintrivir are marked below the ...sequences. ...
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Characterization of a relaxase belonging to the MOBT family, a widespread family in Firmicutes mediating the transfer of ICEs
... histidine residues that coordinate the cationic co- factor on two of the central beta-sheets, RelSt3 as well as Rep_trans structures instead use 3 acidic residues to do so, each located on a different ...
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COMPUTATIONAL UNDERSTANDING OF SELECTIVE MMP INHIBITORS IN COPD
... the active sites of MMP-09, -12, and -14 enzymes. Secondly, the key residues along the S1-pocket have been identified to resolve the structural differences leading to active site shape ...the ...
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Can sequence determine function?
... Database searches disclose that the E. coli genome encodes three members of the enolase superfamily that are most homologous to MR (Figure 3). MR catalyzes a 1,1-proton transfer reaction using Lys166 and His297 of the ...
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Stability activity tradeoffs constrain the adaptive evolution of RubisCO
... Diversification of RubisCO on an Island of Stability. Throughout their evolutionary histories, RubisCO genes have faced signifi- cant changes, both internal and external to the organism, which have altered the ...
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Asymmetric synthesis of amino acid derivatives as molecular probes for the mechanism of 3 methylaspsartate ammonia lyase
... acid residues in the active site were probed by a number of chemical modification ...the active site. In order to identify the site of modification, these experiments were ...
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The crystal structure of superoxide dismutase from Plasmodium falciparum
... SODs are classified according to their metal cofactors. Eukaryotic cells are generally served by a cytosolic Cu/ ZnSOD and an evolutionarily unrelated mitochondrial MnSOD. Some eukaryotes also contain a SOD containing a ...
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Engineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates
... KsdD3 is a FAD-dependent enzyme that catalyzes the formation of a double bond at the C1–2 position of 3-ketosteroid substrates (Fig. 1a). The catalytic activity of KsdDs is highly dependent on the assay conditions [17, ...
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