adenovirus type 2

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Viral DNA sequences and gene products in hamster cells transformed by adenovirus type 2.

Viral DNA sequences and gene products in hamster cells transformed by adenovirus type 2.

Viral DNA Sequences and Gene Products in Hamster Cells Transformed by Adenovirus Type 2 Received for publication 17 April 1978 Complementary strand-specific adenovirus DNA of full length[r]

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Genetic analysis of adenovirus type 2. VIII. Physical locations of temperature-sensitive mutations.

Genetic analysis of adenovirus type 2. VIII. Physical locations of temperature-sensitive mutations.

We have analyzed the DNA structures of many interserotypic recombinants formed in crosses between temperature-sensitive ts mutants of adenovirus type 2 and adenovirus type 5 with the res[r]

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Transfection of KB Cells by Liposomes Containing Adenovirus Type 2 DNA

Transfection of KB Cells by Liposomes Containing Adenovirus Type 2 DNA

1 NOTES Transfection of KB Cells by Liposomes Containing Adenovirus Type 2 DNA Received 16 January 1981/Accepted 8 April 1981 Adenovirus type 2 DNA was entrapped in liposomes which were [r]

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The mechanism of activation of the adenovirus type 2 protease

The mechanism of activation of the adenovirus type 2 protease

The attachment of adenovirus type 2 to cells is mediated by the fiber protein (Londberg-Holm and Phiiipson, 1969), whose distal knob domain at the C-terminal of the protein is thought to bind to a cellular receptor (Devaux et al., 1987), The identity of this receptor is still a mystery, although three cellular membrane polypeptides have been captured on a penton-fiber affinity matrix and shown to inhibit Ad2 attachment to cells (Hennache and Boulanger, 1977). The finding that Ad2 binds to some cells but does not enter them effectively (Silver and Anderson, 1988) suggested the possibility that a second protein-protein recognition event should occur for internalisation, and this has now been identified. The penton base protein binds to specific members of a family of heterodimeric cell surface receptors designated integrins (Wickham et ai., 1993). Yet, the penton-integrin interaction on its own is not sufficient for viral binding to cells, as soluble fiber or fiber antibodies can block adsorption completely. Therefore, adsorption and internalisation is a two-component process which requires the interaction of both fiber and penton proteins with their related cellular targets.
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Domains required for assembly of adenovirus type 2 fiber trimers.

Domains required for assembly of adenovirus type 2 fiber trimers.

Entry of human adenovirus into cells is a two-step process, mediated in the first step by a specific interaction between the trimeric fiber protein and a specific receptor on the surface of susceptible cells. Because of the interest in human adenovirus as a vector for gene therapy, we have mapped domains in the fiber protein that are important for proper assembly of this trimeric structure and for proper addition of O-linked N -acetylglu- cosamine ( O -GlcNAc). Mutants of adenovirus type 2 fiber in this study were expressed in human cells by use of a recombinant vaccinia virus expression system that yielded protein indistinguishable from the fiber produced during adenovirus infection. The N-terminal half of the protein did not appear to influence fiber trimer formation, since deletions up to 260 amino acids (aa) from the N-terminal end as well as in-frame deletions within the shaft of the molecule still allowed trimerization; internal deletions in the shaft between aa 61 and 260 appeared to alter addition of O -GlcNAc, as judged by loss of reactivity to a monoclonal antibody specific for this carbohydrate addition. Deletions from the C terminus of the molecule (as small as 2 aa) appeared to prevent trimer formation. Additions of amino acids to the C-terminal end of the fiber showed variable results: a 6-aa addition allowed trimer formation, while a 27-aa addition did not. These trimer- defective mutants were also relatively less stable, as judged by pulse-chase experiments. Taken together, our results indicate that trimerization of the fiber requires at least two domains, the entire head (aa 400 to 582), and at least the C-terminal-most 15 aa of the shaft.
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Isolation of Two Plaque Variants from the Adenovirus Type 2-Simian Virus 40 Hybrid Population Which Differ in Their Efficiency in Yielding Simian Virus 40

Isolation of Two Plaque Variants from the Adenovirus Type 2-Simian Virus 40 Hybrid Population Which Differ in Their Efficiency in Yielding Simian Virus 40

These variants were similar in that each induced SV40 T antigen in human embryonic kidney cells and contained similar concentrations of nonhybrid adenovirus type 2 virions and adenovirus[r]

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Uncoating of adenovirus type 2.

Uncoating of adenovirus type 2.

Within 1 h of infection, virions were converted into three subviral structures: i subviral structures in the cytoplasm with a density greater than virions but which qualitatively still c[r]

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Biosynthesis of adenovirus type 2 i-leader protein.

Biosynthesis of adenovirus type 2 i-leader protein.

To determine whether the i-leader protein is synthesized during productive infection and to provide an immunological reagent to study the properties and functions of the i-leader protein[r]

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Anatomy of region L1 from adenovirus type 2.

Anatomy of region L1 from adenovirus type 2.

The structure of two cytoplasmic polyA-containing Li RNAs which both retain the VA RNAs as part of their hypothetical 3'-noncodi'ng regions are described; Moreover, it is shown that exte[r]

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Evidence for an Adenovirus Type 2-Coded Early Glycoprotein

Evidence for an Adenovirus Type 2-Coded Early Glycoprotein

In this report, we present evidence that our 21K polypeptide is an Ad2-coded early glycopolypeptide, in that it can be labeled with [3H]glucosamine, it binds to concanavalin A ConA-Sepha[r]

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Termination sites for adenovirus type 2 DNA replication.

Termination sites for adenovirus type 2 DNA replication.

Hybridization of 'H-labeled single strands from heavy DNA with unlabeled L- and H-strand Ad2 DNA Expt Expt % Heavy DNA eluted with 0.14 M phosphate buffer 1 24 2 20 3 4 20 18 aInfected c[r]

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Location of the Origin of DNA Replication in Adenovirus Type 2

Location of the Origin of DNA Replication in Adenovirus Type 2

If one harvests the heavy side of the Ad2+ND, GC-rich half molecules after cesium chloride equilibrium centrifugation and the light half of the Ad2+ND, AT-rich molecules, it can be shown[r]

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Paracrystal Formation in Cell Cultures Infected with Adenovirus Type 2

Paracrystal Formation in Cell Cultures Infected with Adenovirus Type 2

These crystals were formed in a number of different cell types, e.g., LLC-MK2 a stable cell line of rhesus monkey kidney cells, Vero, HeLa, HT, and HE, but the number of cells containing[r]

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Vitronectin receptor antibodies inhibit infection of HeLa and A549 cells by adenovirus type 12 but not by adenovirus type 2.

Vitronectin receptor antibodies inhibit infection of HeLa and A549 cells by adenovirus type 12 but not by adenovirus type 2.

HeLa cells and A549 cells were treated in suspension with monoclonal antibodies specific for integrins cavP5 and cavl33 P1F6 and LM609, respectively, with the Ad2 penton base central fra[r]

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Physical mapping of a large-plaque mutation of adenovirus type 2.

Physical mapping of a large-plaque mutation of adenovirus type 2.

Analysis of the recombinant DNAs by digestion with EcoRI or BamHI restriction endonucleases indicated that, as expected, recombination had occurred in overlapping sequences map position [r]

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Electron Microscopy of Cells Infected with Adenovirus Type 2

Electron Microscopy of Cells Infected with Adenovirus Type 2

Electron microscope studies of Ad 2-infected cells revealed the presence of similar paracrystalline formations in one of two strains of the virus examined.. This report comprises an atte[r]

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Structure of the inverted terminal repetition of adenovirus type 2 DNA.

Structure of the inverted terminal repetition of adenovirus type 2 DNA.

Duplex DNA of 480d3ilV, has the lambdoid phage type cohesive ends of length 12 nucleotides, T7 DNA which has flush ends, duplex Ad2 EcoRI-E fragment, which has staggered ends of 4 nucleo[r]

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Expression of adenovirus type 2 late genes in HeLa cells infected with adenovirus type 2 or adenovirus type 2-simian virus 40 hybrid viruses.

Expression of adenovirus type 2 late genes in HeLa cells infected with adenovirus type 2 or adenovirus type 2-simian virus 40 hybrid viruses.

The possibility that polyadenylation frequencies at these sites may be regulated was tested by examining the expression of the fiber polypeptide IV gene in cells infected with nondefecti[r]

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Bidirectional replication of adenovirus type 2 DNA.

Bidirectional replication of adenovirus type 2 DNA.

24 have shown that the denatured heavy H and light L strands of most of the restriction endonuclease fragments can be successfully separated on agarose gels.. Using the alkaline denatura[r]

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Intermediate in adenovirus type 2 replication.

Intermediate in adenovirus type 2 replication.

Although intermediate DNA sedimented faster than marker viral DNA in neutral sucrose gradients, single strands longer than unit length could not be detected after alkaline denaturation..[r]

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