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Aqueous two-phase system

Purification and characterizations of a novel recombinant Bacillus velezensis endoglucanase by aqueous two-phase system

Purification and characterizations of a novel recombinant Bacillus velezensis endoglucanase by aqueous two-phase system

... NaCl aqueous two-phase system. Aqueous two-phase system is one of the most economical, high-efficient, easy-to-operate, and scale-up methods, which has been ...

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Optimization Of Papain Extraction By Aqueous Two-Phase System (Atps) Using Response Surface Methodology (Rsm)

Optimization Of Papain Extraction By Aqueous Two-Phase System (Atps) Using Response Surface Methodology (Rsm)

... in aqueous two-phase system, defined amounts of solid PEG and sodium carbonate were added to the Crude preparation (30 g) [15] , and the total mixture was made up to 50 g with ...The ...

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Quantification of amino acids and peptides in an ionic liquid based aqueous two-phase system by LC–MS analysis

Quantification of amino acids and peptides in an ionic liquid based aqueous two-phase system by LC–MS analysis

... Aqueous two-phase systems (ATPS) occur by the mixture of two polymers or a polymer and an inorganic salt in ...liquids, Aqueous two-phase system, Partitioning ...

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Potential Recovery of Protein from Shrimp Waste in Aqueous two Phase System

Potential Recovery of Protein from Shrimp Waste in Aqueous two Phase System

... carotenoids. Aqueous two-phase system (ATPS) partitioning has been used to recover and concentrate proteins from Shrimp Waste and offers many advantages along with biomass ...

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Hydrophobic Properties of tRNA with Varied Conformations Evaluated by an Aqueous Two-Phase System

Hydrophobic Properties of tRNA with Varied Conformations Evaluated by an Aqueous Two-Phase System

... stationary phase in a column. In contrast, an aqueous two-phase system (ATPS) is a biocompatible system that can be applied to the partitioning of var- ious biomacromolecules and ...

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Aqueous two-phase system (ATPS): an overview and advances in its applications

Aqueous two-phase system (ATPS): an overview and advances in its applications

... 2DE: Two dimensional electrophoresis; 3D: Three dimensional; ANOVA: Analysis of variance; ATPAP: Aqueous two-phase affinity partitioning; ATPE: Aqueous two-phase ...

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Use of Aqueous Two-Phase System for Partial Purification and Characterization of α-Amylase from Burnatia enneandra Micheli

Use of Aqueous Two-Phase System for Partial Purification and Characterization of α-Amylase from Burnatia enneandra Micheli

... pH system increases the partition coefficient and that the biomolecule is found by affinity in the upper phase (Raja et ...the system (Brockett, ...

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Partitioning of thermostable glucoamylase in polyethyleneglycol/salt aqueous two-phase system

Partitioning of thermostable glucoamylase in polyethyleneglycol/salt aqueous two-phase system

... bottom phase in the systems with different PEGs (MW 1000, 2000, 4000, and 6000) are shown in Table ...bottom phase was greater than that of the top phase, irrespective of the molecular weight of the ...

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Liquid-liquid equilibrium data prediction using large margin nearest neighbor

Liquid-liquid equilibrium data prediction using large margin nearest neighbor

... in aqueous two-phase system ...(LLE) phase diagram behavior is still inadequate and no comprehensive theory exists for the prediction of the experimental ...the phase behavior of ...

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Effective Parameters on the Partition Coefficient of Guanidine Hydrochloride in the Poly (Ethylene Glycol) +Phosphate +Water System at 298.15 K

Effective Parameters on the Partition Coefficient of Guanidine Hydrochloride in the Poly (Ethylene Glycol) +Phosphate +Water System at 298.15 K

... Mixing two or more incompatible polymers or a polymer and a structuring salt in aqueous conditions generally forms an aqueous two- phase system (ATPS), in which the percentage of ...

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Extraction of Cheese Whey Protein from Dairy Effluent by Using Polyethylene Glycol and Sodium Sulphate

Extraction of Cheese Whey Protein from Dairy Effluent by Using Polyethylene Glycol and Sodium Sulphate

... benign aqueous two phase system (ATPS) for the recovery of value added cheese whey ...a two stage ATPS process the effect of system parameters such as pH, types and the ...

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Central composite design application for optimization of aqueous two phase extraction of protein from shrimp waste

Central composite design application for optimization of aqueous two phase extraction of protein from shrimp waste

... Aqueous two-phase system (ATPS) is an effective, environmental friendly and economically viable separation method. ATPS is an ideal technology where clarification, concentration, and partial ...

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Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system

Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system

... an aqueous two-phase system (ATPS) and ATPMS based on Pluro- nic L-35, a thermo-responsive copolymer, in order to se- lectively separate three model proteins: cytochrome c, ovalbumin, and ...

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A Review on Aqueous Two Phase Systems with a Special Mention on Application of Ionic Liquids

A Review on Aqueous Two Phase Systems with a Special Mention on Application of Ionic Liquids

... Abstract: Aqueous two phase system (ATPS) is an excellent biocompatible extraction methodology having wide applications in the purification of various ...ATPS system offers the ...

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Partitioning of Cholesterol Oxidase Produced by Streptomyces olivaceus MTCC 6820 in Different PEG-Salt-Water Aqueous Two Phase Systems

Partitioning of Cholesterol Oxidase Produced by Streptomyces olivaceus MTCC 6820 in Different PEG-Salt-Water Aqueous Two Phase Systems

... of aqueous two phase systems (ATPS) for partitioning of cholesterol oxidase (Chox) produced by Streptomyces olivaceus MTCC 6820 was done using eight different ATPS composed of two different ...

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Fluoroalcohol Mediated Aqueous Two-Phase Systems: Characterization and Applications in Organic Synthesis and Extraction

Fluoroalcohol Mediated Aqueous Two-Phase Systems: Characterization and Applications in Organic Synthesis and Extraction

... derivatization of amines was also carried out in spiked river water (Table 4-7). These results show enhanced enrichment for early eluting amines while the later eluting amines give results nearly identical to the ...

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Rapid One-Step Separation and Purification of Recombinant Phenylalanine Dehydrogenase in Aqueous Two-Phase Systems

Rapid One-Step Separation and Purification of Recombinant Phenylalanine Dehydrogenase in Aqueous Two-Phase Systems

... the phase forming ...the system components, the molecular weight and concentration of polymer, the type and concentration of salt, the ionic strength, the system pH and temperature [12- ...about ...

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A REVIEW ON: SUSTAINED RELEASE TECHNOLOGY

A REVIEW ON: SUSTAINED RELEASE TECHNOLOGY

... the aqueous phase surrounding the site of administration and then partition into the absorbing ...membrane. Two of the most important physicochemical properties of a drug that influence its ...

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Aqueous Two Phase Systems Applied to Partition Proteins from Goat Milk Whey In Nature

Aqueous Two Phase Systems Applied to Partition Proteins from Goat Milk Whey In Nature

... pumping system (LC-20AT), autosampler (SIL-20AHT), column oven (CTO-20A), de- tector per diode array (SPD-M20A) and interface ...reversed phase, the column and parameters utilized were the same as presented ...

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Interfacial Assembly In Aqueous Two Phase Systems

Interfacial Assembly In Aqueous Two Phase Systems

... from aqueous mixtures of two polymers that demix to form two phases, termed aqueous two phase systems ...of two water-rich phases and are therefore excellent candidates ...

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