Cap analysis gene expression
Characterization of Novel Transcripts of Human Papillomavirus Type 16 Using Cap Analysis Gene Expression Technology
... used cap analysis gene expression (CAGE) for high-throughput transcriptome analysis (12–16) CAGE specifically determines transcriptional start sites (TSS) ge- nome wide, by capping 5= ...
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CCL2 enhances pluripotency of human induced pluripotent stem cells by activating hypoxia related genes.
... performed Cap Analysis Gene Expression (CAGE), a transcriptome profiling technique, on libraries cultured with and without CCL2 and observed a consistent up-regulation of hypoxia related ...
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Novel promoters and coding first exons in DLG2 linked to developmental disorders and intellectual disability
... CAGE: Cap analysis gene expression; CEGA: Conserved Elements from Genomics Alignments; CFE: Coding first exon; CGH: Complete genomic hybridization; CNV: Copy number variation; DECIPHER: ...
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The cancer-associated CTCFL/BORIS protein targets multiple classes of genomic repeats, with a distinct binding and functional preference for humanoid-specific SVA transposable elements
... The expression of SVA elements that are transcribed in K562 cells is not affected by BORIS ...unique gene promoters. (A) BORIS ChIP-seq and deep CAGE (Cap Analysis of Gene ...
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Analysis of recombinant adeno-associated virus packaging and requirements for rep and cap gene products.
... the expression of the Rep and Cap proteins required for rAAV ...when cap gene expression was increased, implying that the level of capsid protein is one limiting factor for production ...
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Knockdown of menin affects pre-mRNA processing and promoter fidelity at the interferon-gamma inducible IRF1 gene
... CAGE: cap analysis of gene expression; ChIP: chromatin immunoprecipitation; CoREST: RE1- silencing transcription factor co-repressor; CPE: cytopathic effect; CTD: C- terminal domain; CTSS: ...
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Genome-Wide Analysis of the 5′ and 3′ Ends of Vaccinia Virus Early mRNAs Delineates Regulatory Sequences of Annotated and Anomalous Transcripts
... ⬘ cap and 3 ⬘ poly(A) tail. Gene expression profiles of vaccinia virus (VACV) have been deter- mined with tiling microarrays (3, 48) and by RNA-seq tech- nology ...⬘ analysis, we used three ...
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Top2a identifies and provides epigenetic rationale for novel combination therapeutic strategies for aggressive prostate cancer
... in gene expression between primary and metastatic Myc-CaP tumors (aggressive disease progression), we examined primary (n=3) and metastatic tumor tissue (n=4) collected from tumor bearing ...mice. ...
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Enhancer variants: evaluating functions in common disease
... can cap- ture all regions that physically contact a site of interest, without prior knowledge of the regions that contact that site being necessary [62] (that is, a ‘one-to-all’ ...interaction analysis by ...
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Human transposons are an abundant supply of transcription factor binding sites and promoter activities in breast cancer cell lines
... the Cap Analysis of Gene Expression (CAGE) technology, which maps genome-wide tran- scription start sites (TSS) by identifying mRNA 5′ end clusters ...
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Sustained expression of MCP-1 by low wall shear stress loading concomitant with turbulent flow on endothelial cells of intracranial aneurysm
... and cap analysis of gene expression analysis Using an RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany), total RNA was prepared from ECs loaded on shear stress or kept in a static ...
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Divergent functions of two ancient Hydra Brachyury paralogues suggest specific roles for their C terminal domains in tissue fate induction
... mRNA expression is activated at the same stage during budding, the kinetics of expression ...Molecular analysis of the differentiated animal cap phenotypes induced by HyBra ...Marker ...
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Single-Nucleotide Resolution Mapping of Hepatitis B Virus Promoters in Infected Human Livers and Hepatocellular Carcinoma
... moderate expression levels in a subset of samples ...the cap selectivity of the CAGE technology is very high ...X gene body, especially prominent in HBV-infected primary human hepatocytes and ...
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Genomic Analysis of PIS1 Gene Expression
... PIS1 expression, we mined a ChIP-on- chip database containing information on yeast DNA-binding proteins ...This analysis identified Pho2, Yfl044c, and Ste12 as weak candidate PIS1 promoter binding proteins ...
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Analysis of gene expression in intracranial aneurysms
... in gene expression profile could not fully re- flect the early condition of aneurysm, so it could not fully reflect the pathophysiological process of intracra- nial ...in gene expression ...
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Molecular and classical genetic analysis of penicillin biosynthesis in 'Aspergillus nidulans'
... genetic analysis has revealed that npeD is allelic with palF and that npeC has a phenotype consistent with it being a pal mutant and is either allelic with palA or defines a new pal ...
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Identification of key gene modules for human osteosarcoma by co expression analysis
... Here, WGCNA was applied to investigate 2228 genes of 22 samples that were compromised from a dataset obtained from NCBI, and seven modules were yielded. According to correlation study by network heatmap plot (Fig. 5), ...
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Single mammalian cell gene expression analysis using microfluidics
... triplex qPCRs in parallel, for all four genes, or three of the four (Fig. 4.5). 10 pg/μL liver cDNA was diluted serially by factors of 10 to give three templates for the experiment: 10 pg/reaction, 1 pg/reaction, and 0.1 ...
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Patient-derived bladder cancer xenografts in the preclinical development of novel targeted therapies
... the expression of 118 bladder cancer genes [Figure 2D]. This gene set created by integrating three sources: (1) the 17 genes representing potential targets for therapy, (2) the 68 genes associated to ...
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Gene Expression Analysis Of Cardiomyocytes Differentiated From Human Bone Marrow Derived Mesenchymal Stem Cells
... keeping’ gene glyceraldehydes-3-phosphate dehydrogenase (GAPDH) was used to evaluate the efficiency of reverse ...the expression of 6 significant cardiac ...
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