Cell free protein synthesis system
Stögbauer, Tobias Roland (2012): Experiment and quantitative modeling of cell-free gene expression dynamics. Dissertation, LMU München: Fakultät für Physik
... expected protein yield, necessary template DNA concentration and reaction speed of the particular cell-free system which is generally not ...a cell-free system through a ...
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Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell free in vitro translation RNA replication system by viral protein 3CDpro
... bel (ICN Biochemicals) for 12 hr at 34°C. The excess unincorporated label was removed by dialysis. The sam- ples were introduced into a Slide-a-lyzer (Pierce Endogen) dialysis cassette with a M.Wt cut-off of 10 kD and ...
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Synthesis of Bacteriophage M13-Specific Proteins in a DNA-Dependent Cell-Free System II. In Vitro Synthesis of Biologically Active Gene 5 Protein
... B, Autoradiographs of dried gel slices: a, phage M13 marker proteins, gene 3 protein molecular weight 70,000, and gene 5 protein molecular weight 10,000; b, c, proteins synthesized in vi[r] ...
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Integrated lipase production and in situ biodiesel synthesis in a recombinant Pichia pastoris yeast: an efficient dual biocatalytic system composed of cell free enzymes and whole cell catalysts
... recombinant protein production by the methanol utilization plus ...production system under the stepwise hydrolysis- esterification strategy, the water present in this system is used as a reactant for ...
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Stimulation of Poliovirus Synthesis in a HeLa Cell-Free In Vitro Translation-RNA Replication System by Viral Protein 3CDpro
... Preparation of HeLa cytoplasmic extracts. HeLa S10 extracts were prepared as previously described (7, 22), except for the following modifications: (i) packed FIG. 1. Genomic structure of poliovirus and processing of the ...
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In Vitro Synthesis of Turnip Yellow Mosaic Virus Coat Protein in a Wheat Germ Cell-Free System
... To analyze the in vitro synthesized products extract was prepared essentially as described by on sodium dodecyl sulfate SDS-polyacrylamide Davies and Kaesberg 5, with a few modifications[r] ...
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Synthetic Biology Goes Cell-Free
... of protein–protein interactions and the resulting complexes that these interactions can ...at synthesis of such ...the cell-free expression of hepatitis B core antigen VLP (2 subunits) ...
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The influence of the penetrating cryoprotectors on protein synthesis in the cell-free system of the rat liver
... tivity of postmitochondrial supernantant of the rat liver as well as on aminoacylation processes has been investigated. The addition of the penetrating cryoprotectors – ethylene glycol and DMSO – re sulted in the ...
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A User’s Guide to Cell-Free Protein Synthesis
... to cell-extract-based CFPS kits, the PURExpress kit is comprised of a reconstitution of purified components of the transcription and translation machinery from ...(protein synthesis using recombinant ...
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Protein synthesis in a cell free human reticulocyte system: ribosome function in thalassemia
... In the present investigation, a cell free system for protein synthesis has been developed from human reticulocytes and this latter hypothesis tested further by comparing the capacity for[r] ...
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Growth in the slow lane: protein metabolism in the Antarctic limpet Nacella concinna (Strebel 1908)
... compensate protein synthesis rates at low water temperatures, with the trade-off for these modifications being a loss of function at even slightly elevated ...1°C, protein synthesis rates ...
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Maier, Kevin (2012): Design of an acid labile traceless-cleavable click linker for use in a novel protein transduction shuttle. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... test system should provide a platform that is transferable to as much other proteins as ...average protein size and surface ...The protein delivery test system developed in this thesis is ...
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Antibiotics and resistance
... Mechanism of action include: Inhibition of cell wall synthesis Inhibition of protein synthesis Inhibition of nucleic acid synthesis Inhibition of metabolic pathways Interfere[r] ...
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Gene product of region E4 of adenovirus type 5 modulates accumulation of certain viral polypeptides.
... The defects in viral DNA replication, late protein synthesis, and shutoff of host cell protein synthesis associated with the phenotype of the H2dl8O8 mutant were not observed in HeLa cel[r] ...
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Peptide mapping characterization of viral proteins generated in a cell-free coupled system for the transcription and translation of influenza virus mRNA.
... In the coupled cell-free system for transcription and translation of the influenza mRNA's, using the fowl plague virus, we could show the synthesis of virion proteins M and NP and two pe[r] ...
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Evaluation of antibody responses to the early transcribed membrane protein family in Plasmodium vivax
... (Cell- Free Sciences, Matsuyama, Japan) for protein expression using in-fusion cloning (Clontech, Palo Alto, CA, ...tion system (Viogene, Taipei, Taiwan) and were used for protein ...
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Replicative RNA synthesis and nucleocapsid assembly in vesicular stomatitis virus-infected permeable cells.
... Perneable cells represent the only in vitro system described so far in which unequivocal genome replication and nucleocapsid synthesis have been shown to occur.. Previous cell-free RNA-s[r] ...
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Unilateral Synthesis of Reovirus Double-Stranded Ribonucleic Acid by a Cell-Free Replicase System
... A "hybridization-competition" experiment in which the replicase product was denatured and annealed in the presence of an excess of plus-RNA indicated that the in vitro replicase reaction[r] ...
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Effects of temperature and lipophilic agents on poliovirus formation and RNA synthesis in a cell-free system.
... In view of the proposed role of membranes in poliovirus replication in vivo, and a report of an inhibitory effect of oleic acid 9-octadecenoic acid on poliovirus RNA replication 9, we ha[r] ...
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Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications
... self-destruction system which is based on sequence specific and unspecific parts is the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated (Cas) system - the RNA-mediated ...
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