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Cell seeding

Abstract: Electrostatic endothelial cell seeding has evolved as an exceptional technique to improve

Abstract: Electrostatic endothelial cell seeding has evolved as an exceptional technique to improve

... endothelial cell seeding has evolved as an exceptional technique to improve the efficiency of cell seeding in terms of frequency of attached cells and the amount of cell adhesion for ...

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Flow perfusion rate modulates cell deposition onto scaffold substrate during cell seeding

Flow perfusion rate modulates cell deposition onto scaffold substrate during cell seeding

... static seeding, cells were injected from the top of the cylindrical chamber and they travelled down towards the scaffold due to gravity with a constant velocity of ...of cell seeding efficiency was ...

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The effect of concentration, cooling rate and cell seeding density on the initial mechanical properties of agarose hydrogels

The effect of concentration, cooling rate and cell seeding density on the initial mechanical properties of agarose hydrogels

... Investigations of cellular mechanobiology and mechanotransduction pathways in three dimensional hydrogel cultures requires accurate quantification of the material behaviour of the hydrogel, and an understanding of how ...

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Biocompatibility testing of resorbable materials using improved in-vitro techniques

Biocompatibility testing of resorbable materials using improved in-vitro techniques

... using cell lines were more reproducible than in-vitro assays using primary ...of cell lines to test one agent or one response allows the quantification of the results in real terms and comparison with other ...

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A thermoresponsive and magnetic colloid for 3D cell
expansion and reconfiguration

A thermoresponsive and magnetic colloid for 3D cell expansion and reconfiguration

... surfaces), cell growth was monitored by recording fl uorescence images up to day 16 of incubation, using rhodamin-labeled MPSMs to pro- vide contrast against the GFP-expressing ...

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Nonlinear Computational Model of  Biological Cell Proliferation and  Nutrient Delivery in a Bioreactor

Nonlinear Computational Model of Biological Cell Proliferation and Nutrient Delivery in a Bioreactor

... mum cell density with uniform distribution of cells in a final ...of cell proliferation and nutrient supply in a ...non-linear cell proliferation in a bioreactor is developed. A cell seeded ...

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Scaffolds in Periodontal Regeneration

Scaffolds in Periodontal Regeneration

... Most cell-seeding scaffolds are fabricated from two classes of biomaterials, derived from either syn- thetic or natural ...permitting cell interactions, but have the disadvantages of being derived ...

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Biochemical Markers of the Mechanical Quality of Engineered Hyaline Cartilage

Biochemical Markers of the Mechanical Quality of Engineered Hyaline Cartilage

... modulus) from engineered constructs cultured for either, 20, 30, 40 or 80 days was found to positively correlate with GAG, collagen I and collagen II content. The Young’s modulus is determined by unconfined compression ...

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Effect of static seeding methods on the distribution of fibroblasts within human acellular dermis

Effect of static seeding methods on the distribution of fibroblasts within human acellular dermis

... the seeding of cells into the scaffolds. Ef- fective cell seeding can be critical for the successful clinical application of tissue-engineered products ...

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Development and Optimization of a High-Throughput Assay To Measure Neutralizing Antibodies against Clostridium difficile Binary Toxin

Development and Optimization of a High-Throughput Assay To Measure Neutralizing Antibodies against Clostridium difficile Binary Toxin

... time, cell-seeding density, toxin intoxication time, and the ratio of CDTa to a_CDTb in the binary ...Five cell-seeding densities (500, 750, 1,000, 1,500, and 2,000 cells/well), three toxin ...

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Effect of seeding on sucrose polymorphism

Effect of seeding on sucrose polymorphism

... The study of polymorphism in pharmaceutical compounds and their intermediates is very important and an integral part of drug development in present days. In fact, a detailed polymorphic study helps to resolve problems ...

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Seeding forage and weed control

Seeding forage and weed control

... spacing. Seeding rates were based on the recommendations of seed suppliers (table ...to seeding, weeds were controlled with applications of glyphosate at 2 pints per acre (Roundup WeatherMax, ...after ...

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Analysing BitTorrent's seeding strategies

Analysing BitTorrent's seeding strategies

... the seeding strategy on the resistance to freeriders in BitTorrent has been largely ...popular seeding strategies, the Original Seeding Strategy (OSS) and the Time- based Seeding Strategy ...

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Structure of A Crop and Productivity of a Castor-oil Plant Depending on Periods of Sowing, Norms of Sowing and Seeding Depth

Structure of A Crop and Productivity of a Castor-oil Plant Depending on Periods of Sowing, Norms of Sowing and Seeding Depth

... with seeding-down depth of 7-8 ...when seeding according to the scheme 90E60 cm, seeding rate of 20 thousand pieces of fertile seeds per 1 ha, that results in 18,8 centner/ha of oil seeds of a ...

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Tissue-engineered cardiac patch seeded with human induced pluripotent stem cell derived cardiomyocytes promoted the regeneration of host cardiomyocytes in a rat model

Tissue-engineered cardiac patch seeded with human induced pluripotent stem cell derived cardiomyocytes promoted the regeneration of host cardiomyocytes in a rat model

... Explanted cardiac patches were fixed in 4% para- formaldehyde, embedded in paraffin, sliced (5 μm thick sections), and stained with Hematoxylin and Eosin (H&E). H&E staining was used for cell counting. One ...

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) of cisplatin: a pilot study on the artifacts of the MTT assay and the precise measurement of density-dependent chemoresistance in ovarian cancer

) of cisplatin: a pilot study on the artifacts of the MTT assay and the precise measurement of density-dependent chemoresistance in ovarian cancer

... direct cell viability tests ...cancer cell line [10, 53] and found that different IC 50 detection systems generate severe and irreconcilable contradictions in IC 50 data between large- scale international ...

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Design and evaluation of a maize monitoring system for precision planting

Design and evaluation of a maize monitoring system for precision planting

... photoelectric cell and visual sensor are the two main types of sensors, which are sensitive to the shapes of the kernels dropping though the seed meters in bench-top laboratory ...photoelectric cell, mainly ...

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Design and performance evaluation of PHilMech multi-row onion seeder:  Ten-row model

Design and performance evaluation of PHilMech multi-row onion seeder: Ten-row model

... Similarly, the multi-row onion seeder had higher field capacity in the production areas of Pangasinan (0.37 ha h -1 ) than manual broadcasting (0.35 ha h -1 ) (Table 6) while the seeding rate of the machine (4.94 ...

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Optimized design and performance evaluation of an electric cup-chain potato metering device

Optimized design and performance evaluation of an electric cup-chain potato metering device

... Based on the drive type, cup style can be divided into cup-chain and cup-belt. Cup-belt is often utilized in large tractors because of its strong demand for power, as demonstrated by the highly reliable German Grimme GL ...

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When can overambitious seeding cost you?

When can overambitious seeding cost you?

... overambitious seeding of social ...overambitious seeding can result in people adopting in suboptimal contexts, having bad experiences, and abandoning the product before more favorable contexts for adoption ...

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