cleavage/packaging
The Essential Human Cytomegalovirus Gene UL52 Is Required for Cleavage-Packaging of the Viral Genome
... A central question is how pUL52 contributes to cleavage and/or packaging of the viral genomes. We expected that the characterization of the protein and its subcellular localization might give us some hints. ...
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Functional Identification and Analysis ofcis-Acting Sequences Which Mediate Genome Cleavage and Packaging in Human Herpesvirus 6
... for cleavage and packaging of unit length viral ...herpesvirus cleavage-packaging signals, pac1 and pac2, are located at the left and right genomic termini of human herpesvirus 6 (HHV-6), ...
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Intracellular Cre-Mediated Deletion of the Unique Packaging Signal Carried by a Herpes Simplex Virus Type 1 Recombinant and Its Relationship to the Cleavage-Packaging Process
... (HSV-1) packaging signal (a se- quence), we constructed a recombinant virus containing a unique a sequence, which was flanked by two loxP sites in parallel ...the cleavage-packaging ...the ...
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The Human Cytomegalovirus UL51 Protein Is Essential for Viral Genome Cleavage-Packaging and Interacts with the Terminase Subunits pUL56 and pUL89
... replication, cleavage- packaging, maturation, and nuclear egress of capsids) are thought to be tightly linked, it is possible that protein-protein interactions within such an encapsidation network are only ...
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The Varicella-Zoster Virus Portal Protein Is Essential for Cleavage and Packaging of Viral DNA
... Beyond the utility of portal mutants as single-cycle vaccines, our laboratory is interested in the structure and function of her- pesvirus portal proteins. The phenotypic effect of inhibiting pORF54 function was ...
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Human Cytomegalovirus pUL93 Is Required for Viral Genome Cleavage and Packaging
... genome cleavage and packaging and it could also be necessary for maintaining capsid stability after genome cleav- age and ...and packaging of viral DNA are believed to occur simultaneously (25–29), ...
5
Herpes Simplex Virus DNA Cleavage and Packaging Proteins Associate with the Procapsid prior to Its Maturation
... DNA cleavage and packaging machin- ...at packaging during early stages of capsid ...DNA cleavage and packaging in greater detail in a defined in vitro ...
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Kaposi's Sarcoma-Associated Herpesvirus ORF68 Is a DNA Binding Protein Required for Viral Genome Cleavage and Packaging
... DNA cleavage in ...DNA cleavage. Similar to the DNA binding activity, our in vitro cleavage reactions do not capture TR-specific targeting, as we found that other nonviral DNA was similarly ...
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Sequences within the Herpesvirus-Conservedpac1 and pac2 Motifs Are Required for Cleavage and Packaging of the Murine Cytomegalovirus Genome
... in cleavage and packaging was dem- onstrated by a mutation in the left poly(C) region that virtually eliminated cleavage and packaging at the ectopic ...reduced cleavage and ...
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Herpes Simplex Virus Type 1 Cleavage and Packaging Proteins UL15 and UL28 Are Associated with B but Not C Capsids during Packaging
... various cleavage and packaging proteins can be proposed based on the results pre- sented in this paper taken together with other ...that cleavage and packaging occur within replication ...
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DNA Cleavage and Packaging Proteins Encoded by Genes UL28, UL15, and UL33 of Herpes Simplex Virus Type 1 Form a Complex in Infected Cells
... The cleavage and packaging process of herpesviruses is be- lieved to be similar to that employed by double-stranded DNA bacteriophages; consequently, it is useful to consider the mech- anisms used by these ...
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Functional domains within the a sequence involved in the cleavage-packaging of herpes simplex virus DNA.
... The presence of these repeat units in the serially passaged virus stocks was surprising in that, as already noted above, we did not encounter chimeric repeat units containing inserted he[r] ...
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Mutational Analysis of the Herpes Simplex Virus Type 1 DNA Packaging Protein UL33
... by cleavage at the packaging signal can be observed in samples of total DNA in the absence of detect- able DNase-resistant DNA (21, ...the cleavage-packaging pro- cess (4, ...the ...
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Dissecting the roles of VP0 cleavage and RNA packaging in picornavirus capsid stabilization: the structure of empty capsids of foot-and-mouth disease virus.
... the cleavage products of the protein precursor ...VP0 cleavage confers stability on the picornavirus capsid over and above that attributable to RNA ...the cleavage of ...
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A host cell protein binds to a highly conserved sequence element (pac-2) within the cytomegalovirus a sequence.
... Because experiments with HSV-1 indicated that pac-1 and pac-2 motifs were essential elements in the cleavage/packaging of that virus, we tested whether a small region of the CMV a sequen[r] ...
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Helper virus-free transfer of herpes simplex virus type 1 plasmid vectors into neural cells.
... virus-free packaging system for these vectors, the DNA cleavage/packaging signals were deleted from a set of cosmids that represents the HSV-1 ...and packaging of vector DNA. However, in the ...
8
Excision of DNA fragments corresponding to the unit-length a sequence of herpes simplex virus type 1 and terminus variation predominate on one side of the excised fragment.
... These observations are interpreted to mean that the cleavage-packaging system functions adequately on the DR1 second distal from the S component, and cleavages of other DR1 elements are [r] ...
7
Nucleotide sequence and structural features of a novel US-a junction present in a defective herpes simplex virus genome.
... RESULTS The present study was undertaken to determine the nucleotide sequence of a functional cleavage-packaging signal within defective HSV-1 DNA and to define the structural organizati[r] ...
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Epstein-Barr Virus BALF3 Has Nuclease Activity and Mediates Mature Virion Production during the Lytic Cycle
... DNA cleavage and packaging occur at specific sites in the ...DNA cleavage and packag- ing ...DNA cleavage and packaging (39), and HCMV UL56 appears to bind to pac1 and pac2 motifs and ...
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Study of herpes simplex virus maturation during a synchronous wave of assembly.
... DNA packaging (Fig. 4). Packaging is tightly coupled to cleavage of head-to-tail con- catameric viral DNA to genome-length pieces, generating dis- crete termini ...virus. Packaging appeared to ...
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