Cloning and sequencing analysis
Detection and Identification of Mycobacterium tuberculosis in Joint Biopsy Specimens by rpoB PCR Cloning and Sequencing
... M. tuberculosis is readily differentiated by its rpoB sequence. In addition, important information on rifampin susceptibility can be provided by rpoB sequence analysis, which is impossible by IS6110 PCR or 16S ...
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A High-Throughput Method for Cloning and Sequencing Human Immunodeficiency Virus Type 1 Integration Sites
... Sequencing analysis and mapping of integration sites by use of the high-throughput Int-tag assay. To test the new high- throughput method, the identical DNA sample isolated from CEM cells infected with ...
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Cloning, Sequencing and Phylogenetic Analysis of Stn Gene of Salmonella Typhimurium
... (Received: 15 December 2017; accepted: 28 December 2017) Salmonella Typhimurium is an important facultative bacillus pathogen having broad host range and high physiological adoptability. Several genes contribute in ...
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Cloning and Sequencing the First HLA Gene
... DNA sequencing at the Laboratory of Molecular Bi- ology, Cambridge (UK), with a close associate of Fred Sanger, George Brownlee, who, strangely enough, was not using Sanger sequencing (S anger et ...direct ...
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Cloning, sequencing, and functional analysis of a Marek's disease virus origin of DNA replication.
... Neither the replication origins of Epstein-Barr virus (oriP and oriLyt) nor those of cytomegalovirus contained sequences similar to the putative origin of serotype 2 MDV replication (1, [r] ...
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Investigating a Eukaryotic Genome: Cloning and Sequencing a Fragment of Yeast DNA
... Analysis of DNA fragments recovered is designed to gain some insight into the organization of eukaryotic genomes. Educational Context This lab was created to accompany lecture topics in genomics, bacterial ...
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CHAPTER 6. CLONING AND SEQUENCING OF CCCVd-RELATED RT-PCR PRODUCTS
... The occurrence of these variants agrees with the RPA analysis (Chapter 4), which showed that the CCCVd-like RNAs in this palm had several mismatches when compared with CCCVd. The presence of sequences equivalent ...
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Cloning and Sequencing of a Candida albicans Catalase Gene and Effects of Disruption of This Gene
... sequence analysis revealed an open reading frame encoding a protein of 487 amino acid ...Enzymatic analysis revealed an abundance of catalase in the wild-type strain but decreasing catalase activity in ...
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Cot based cloning and sequencing of the short arm of wheat chromosome 1B
... ABSTRACT Sequencing of cereal genomes is not a feasible task due to their large size and high content of repetitive DNA se- ...simplify analysis of such genomes: reduced representation approaches, such as ...
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Five-minute cloning of Taq polymerase-amplified PCR products for sequencing
... Before Starting For each transformation, prepare two LB plates containing 50 µ g/ml kanamycin. Note: Do not use plates containing ampicillin. The control template is a plasmid that encodes ampicillin resistance. This ...
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Comparison of two methods of generating random DNA fragments for cloning and sequencing
... Many thanks to Dr. Oary Olsen, the overseer of this scientific foray, for his helpful input and numerous rough draft revisions. Also, thanks to Chris Olson, Amy Whetsell, and Gisbert Meuter, my cohabitants in the Center ...
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Molecular cloning, sequencing, and expression of Eimeria tenella HSP70 partial gene.
... After selecting the region of highest hydrophilicity in the hsp70 gene, we cloned complementary DNA (cDNA) into a pTrcHis2-TOPO vector and transformed it into TOP10 Escherichia coli cells; after induction, the bacteria ...
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IDENTIFICATION, ISOLATION, CLONING AND SEQUENCING APARTIALANNEXIN GENE FROM AUREOBASIDIUM PULLULANS
... A range of web-based tools were used for analysis of DNA and protein sequences. Novel DNA sequences were submitted for analysis through the Basic Local Alignment Search Tool (Basic BLAST version 2.0). BLAST ...
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Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad range PCR, cloning and sequencing
... PCR, sequencing and cloning are essential tech- niques for the characterization of the microbial environment in ...joint. Cloning procedures allowed the identifi- cation of known ReA-triggering ...
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Efficient Mapping and Cloning of Mutations in Zebrafish by Low-Coverage Whole-Genome Sequencing
... and analysis of mutants in zebrafish has been instrumental in defining the genetic regulation of vertebrate development, physiology, and ...the analysis of mutants. Whole-genome sequencing has recently ...
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Genome sequencing accuracy by RCA-seq versus long PCR template cloning and sequencing in identification of human papillomavirus type 58
... reported sequencing accuracy for a NGS with Illumina HiSeq platform is 98% [37], we found that RCA-seq in this report displayed a more reliable ap- proach, by calling consensus bases, in decoding the entire HPV58 ...
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Cloning and Expression Analysis of GmCYP78A5 Promoter
... The positive clone plasmid identified by enzyme digestion was sent to Takara company for sequencing. The result showed that the gene fragment was about 1650 bp, which had 99.21% homology with the promoter sequence ...
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High efficiency RNA cloning enables accurate quantification of miRNA expression by deep sequencing
... expression analysis by qRT- PCR and microarray usually characterizes differential expression levels of individual miRNAs and identifies miRNAs with the largest fold changes from two or more ...our analysis ...
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Characterization of a circulating PRRSV strain by means of random PCR cloning and full genome sequencing
... 3 ProVaxs, Faculty of Medicine and Health, Ghent University, De Pintelaan 185 - 3K3, 9000 Gent, Belgium. Authors ’ contributions JVD carried out the molecular characterization and drafting of the manuscript. MG isolated ...
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Cloning and sequencing of complete τ-crystallin cDNA from embryonic lens of Crocodylus palustris
... the cloning and sequencing of complete τ-crystallin cDNA from the embryonic lens of Crocodylus palustris and establish it to be identical to the α-enolase gene from non-lenticular ...reconstruction ...
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