confocal microscope
A stage scanning laser confocal microscope and protocol for DNA methylation sequencing
... In this communication, we report the customized in- strument and protocol for fluorescence detection of DNA bands, reporting methylation status in a target gene. An automated stage-scanning laser confocal ...
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Three-dimensional detection of sequence of strokes using confocal microscope
... A1 confocal microscope is a versatile instrument for the analysis of the sequence of ...the confocal microscope assisted in the examination process, especially the three-dimensional im- ages ...
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In-Situ Study of Single and Double Loop Reactivation Methods During the Characterisation of the Degree of Sensitisation of a Duplex Stainless Steel (UNS 1.4462) Using a Minicell and a Confocal Microscope
... The aim of this work is to study the evolution of the electrode surface during the single and double reactivation methods at microscopic scale. The tested material has been a duplex stainless steel (UNS 1.4462) in its ...
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Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal
... We demonstrated the in-situ observation of a moving atomic force microscope (AFM) cantilever using a laser confocal microscope combined with a differential interference microscope (LCM-DIM). ...
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Designing a laser scanning confocal microscope: a new scan engine layout
... Imaging has been around for ages. Since the evolution of the first light detecting cells organisms have been able to notice the world through vision. As time progressed the eye evolved, using lenses to be able to focus ...
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Dentine permeability, adhesive penetration and interfacial stress: A confocal microscope study
... Teeth, maintained in near physiologic conditions, were restored with a dentine bonding system, in different cavity configurations and interfacial regions examined using fluorescence confocal microscopy (tandem ...
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Quantification of Plastic Remnants on Titanium Surface after Instrumentation and Evaluation of Efficacy of Its Removal after Irrigation by using a Confocal Microscope
... Bertoldi et al in 2015 evaluated changes to titanium implants smooth surfaces after instrumentation using low vacuum scanning electron microscope (LV-SEM) and white light confocal (WLC) profilometry. The ...
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PubMedCentral-PMC5437170.pdf
... upright confocal configuration equipped with a water immersion “dipping” lens to image growing SAMs in plants temporarily submerged in an aqueous medium within a chamber (Prunet et ...
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ZIP4, a Novel Determinant of Tumor Invasion in Hepatocellular Carcinoma, Contributes to Tumor Recurrence after Liver Transplantation
... Figure 1. (A) Laser-scanning confocal microscope was used to detect the distribution of ZIP4 in HepG2, BEL7402, and HL-7702 cells (magnification: ×1260). Cells were incubated in a cell culture dish, fixed ...
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Measurement of Protein 53 Diffusion Coefficient in Live HeLa Cells Using Raster Image Correlation Spectroscopy (RICS)
... Correlation Spectroscopy (RICS), which enables to measure the protein dynamics in a living cell by using commercial laser scanning confocal microscope without adding additional expensive components. The ...
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Superresolution fluorescence microscopy for 3D reconstruction of thick samples
... scan confocal microscope was built upon a com- mercial inverted microscope (IX71, ...the microscope as well as to send fluores- cence signal from a sample to an EMCCD camera (ProEM, Princeton ...
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The effects of simvastatin on cellular viability, stemness and osteogenic differentiation using 3-dimensional cultures of stem cells and osteoblast-like cells
... Objectives. The aim of the study was to evaluate the effects simvastatin has on cellular viability, stemness and osteogenic differentiation using 3-dimensional cell spheroids of stem cells and osteoblast-like cells. ...
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Confocal microscopy
... a confocal microscope is well- collimated and stationary makes it easy to implement a grating or prism spectrograph in the emission path, and improve the flexibility of the spectral windowing of the ...in ...
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Original Article Renalase overexpression in ER-positive breast cancer
... For immunofluorescent staining, sections were blocked and incubated overnight at 4°C with primary antibodies as mentioned above. The dilution of the primary antibodies against re- nalase (clone: ERP4212, Abcam, ...
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In vivo slit scanning confocal microscopic observation in a patient with moderate and severe keratoconus: a case report
... Confocal microscope has the potential to play a major role in the evaluation and long-term monitoring of corneal micro- structure in keratoconus patients. In the above discussed case, corneal cell ...
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Analysis of plant tissue images obtained by confocal tandem scanning reflected light microscope
... Immediately after cutting, the sample was rinsed in distilled water in order to remove starch and other cell components that potentially remained on the surface. Next, the slice was mounted on a microscopy slide and ...
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Darkfield-Confocal Microscopy detection of nanoscale particle internalization by human lung cells
... co-localized confocal and darkfield ...the confocal microscope in the green channel while scattered incident light collected by the darkfield condenser was simultaneously observed via the transmitted ...
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Design of a Cascaded Black – Linear Distribution (CBLD) in Circular Aperture and Its Application on Confocal Laser Scanning Microscope (CLSM)
... of confocal microscopy was patented by Marvin Minsky in 1957 but it took a good few years before it was fully developed to incorporate a laser scanning ...conventional microscope you can only see as far as ...
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3D visualization of extracellular vesicle uptake by endothelial cells
... For confocal examinations, TIME cells and HUVECs were cultured in glass bottom dishes to reach 50% ...710 confocal laser microscope with an oil-immersion Plan-Apochromat 40x NA ...
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Inverted Fluorescence Microscope
... inverted microscope [1]. The microscope has capabilities such as a large field of view at 25 mm and a Z-axis stabilizer to reduce vibrations in the system to maintain focus on the ...
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