Conventional-RT
Development of one step SYBR Green real time RT PCR for quantifying bovine viral diarrhea virus type 1 and its comparison with conventional RT PCR
... I RT-PCR was developed by amplifying cDNA template from viral RNA and using in vitro transcribed BVDV RNA to establish a standard ...than conventional RT-PCR and can quantitatively detect BVDV RNA ...
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Identification of Dengue Virus in Respiratory Specimens from a Patient Who Had Recently Traveled from a Region Where Dengue Virus Infection Is Endemic
... real-time RT-PCR, were tools used for ...infection. Conventional RT-PCR and sequencing, in addition to real-time RT-PCR of serum samples and nasal and throat swabs from the patient, confirmed ...
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Comparison of SYBR green I real time RT PCR with conventional agarose gel based RT PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco
... real-time RT-PCR targeting the IBV N gene would also be positive using a one-step RT-PCR target- ing the IBV S1 ...one-step RT-PCR. The obtained RT- PCR product, 700 bp, corresponded to the ...
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Real Time Reverse Transcriptase PCR Assay for Detection of Human Metapneumoviruses from All Known Genetic Lineages
... real-time RT-PCR assays have a very high level of ...conducted conventional RT-PCR analysis or virus isola- ...real-time RT-PCR ...real-time RT- PCR ...
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... accelerated RT arm than those of conventional fractionation ...to conventional arm (89% ...to conventional arm (99% ...accelerated RT arm compared to conventional RT (53% ...
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Evaluation and Validation of Real Time Reverse Transcription PCR Assay Using the LightCycler System for Detection and Quantitation of Norovirus
... quantitative RT-PCR systems are slowly re- placing conventional RT-PCR assays for the detection and quantification of RNA viruses in research and diagnostic lab- ...real-time RT-PCR assays for ...
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Development of One Step, Real Time, Quantitative Reverse Transcriptase PCR Assays for Absolute Quantitation of Human Coronaviruses OC43 and 229E
... Conventional RT-PCR screening of a collection of 342 respi- ratory samples from patients hospitalized with relatively severe respiratory tract infections revealed seven HCoV OC43-infected samples ...by ...
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Practice patterns for the radical treatment of nasopharyngeal cancer by head and neck oncologists in the United Kingdom.
... Conventional RT target volumes for NPC are large in the UK, in which the entire nasopharyngeal compart- ment, retropharyngeal lymph nodes (RPN) and involved nodal levels are treated to a radical dose of 65 ...
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Design for Stable Lasing of an Indirect Injection THz Quantum Cascade Laser Operating at Less Than 2 THz
... lengths of 1.5 mm and measured them using a cryogenic Si- bolometer with an FT-IR system in pulsed mode. Figure 2(a) shows the measured emission spectrum at 5 K. Lasing is at 7.85 meV, corresponding to a frequency of ...
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Use of TaqMan Real Time Reverse Transcription PCR for Rapid Detection, Quantification, and Typing of Norovirus
... B conventional duplex ...by conventional RT-PCR with primers Mon 431 and Mon 433 for genogroup I and primers Mon 432 and Mon 434 for genogroup II (Table ...B RT-PCR mixture consisted of 1 l ...
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Multiplex Real Time PCR Assay for Detection of Influenza and Human Respiratory Syncytial Viruses
... second RT-PCR test targeting a conserved gene (M2) of influenza A viruses was performed as reported previously ...the conventional RT- PCR assay in 22 ...M2 RT-PCR assay as the reference test, ...
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Development of a Microplate Lectin Capture RT PCR (MLC RT PCR) for the Detection of Avian Infectious Bronchitis Virus
... MLC- RT-PCR, although the interactions of Con A with mo- lecular components of these viruses can not be ruled ...with conventional RT-PCR, or with the virus isolation method in embryonated SPF ...
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Broadly Reactive and Highly Sensitive Assay for Norwalk Like Viruses Based on Real Time Quantitative Reverse Transcription PCR
... all RT-PCR ...EM. Conventional RT-PCR methods did not detect other RNA viruses, such as hepatitis A virus, hep- atitis E virus, poliovirus, Sapporo-like viruses, and Astro virus (data not ...
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Development and Evaluation of a Multitarget Real Time Taqman Reverse Transcription PCR Assay for Detection of the Severe Acute Respiratory Syndrome Associated Coronavirus and Surveillance for an Apparently Related Coronavirus Found in Masked Palm Civets
... by conventional RT-PCR targeting the M gene of SARS-CoV, we saw that one specimen (specimen MPC200404085) was positive in all tests with the three primer and probe sets, while for the rest of the speci- ...
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Development of Group and Serotype Specific One Step SYBR Green I Based Real Time Reverse Transcription PCR Assay for Dengue Virus
... by RT-PCR and dengue virus antigen de- tection by ELISA (1, ...various RT-PCR protocols for dengue virus identification (8, 9, 10, 14, 17, 19, ...nested RT-PCR protocols originally reported by ...
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Diagnosis of Rabies via RT PCR on Skin Samples of Wild and Domestic Animals
... In veterinary medicine, IF techniques on skin specimens of rabid animals showed promising results [8] [11] [24]-[26], but were never routinely used, probably due to the expensive equipment needed for cryosectioning and ...
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Simulation Analysis of Solution Transportation Absorption Chiller with the Capacity from 25 RT to 1000 RT
... In this study, we examined the large-scale cooling power STA on simulation. The examination cooling powers were from 90 kW (25RT) to 3517 kW(1000RT). All cooling power achieved around COP 0.64 including pump power ...
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Survival analyses correlate stanniocalcin 2 overexpression to poor prognosis of nasopharyngeal carcinomas
... In the cases included in this study, the choice of initial treatment was made by physicians’ recommendation and patients’ preference. As a retrospective study, we explored whether STC2 overexpression correlated to NPC ...
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COMPARISON IMPACT OF CONVENTIONAL VERSUS CONFORMAL RADIOTHERAPY TECHNIQUES ON THYROID IN BREAST CANCER IRRADIATION
... different RT techniques on thyroid gland; group (I) conventional two-dimensional radiotherapy of SC node and Group (II) three-dimensional conformal radiation therapy (3DCRT) planning of SC ...SC RT, ...
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Long term effects of different soil tillage systems on maize (Zea mays L ) yields
... no-tillage, RT – reduced tillage and CT – conventional ...the RT treatment, tillage was performed with a rotavator in the autumn to a depth of 10–12 ...
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