Cytoplasmic RNA
Cytoplasmic RNA viruses as potential vehicles for the delivery of therapeutic small RNAs
... retroviruses/lentiviruses. Cytoplasmic RNA viruses have been excluded as viral vectors for RNAi therapy because of the nuclear localization of the microprocessor complex and the potential degradation of the ...
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Nucleoside Modifications Suppress RNA Activation of Cytoplasmic RNA Sensors
... in RNA reduces the activation of RNA-sensing TLRs and retinoic acid inducible gene I (RIG-I), which initiate signaling cascades following activation and result in transcription of pro-inflammatory ...two ...
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A Cytoplasmic RNA Virus Alters the Function of the Cell Splicing Protein SRSF2
... total RNA was harvested, the contaminating DNA was removed, and an aliquot of the RNA was converted to cDNA for qRT-PCR to confirm T1L repression of IFN-  signaling (data not ...Total RNA samples ...
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THE ROLE OF SULFHYDRYL GROUPS IN RNA METABOLISM EUR 1875 e
... Increase in cytoplasmic RNA.-If one studies the relative increase of 3 Hcytidine in cytoplasmic RNA, irrespective of the fact that some "RNA" has been lost during label transfer from the[r] ...
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Transcription map for adenovirus type 12 DNA.
... To map the viral DNA sequences complementary to late RNA, 3H-labeled 1- and r-strand viral DNA sequences selected by hybridization to late cytoplasmic RNA were hybridized to DNA fragment[r] ...
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In vitro synthesis of adenovirus type 5 T antigens. I. Translation of early region 1-specific rna from lytically infected cells.
... Lane 1, proteins synthesized with early cytoplasmic RNA hybridized to total Ad5 DNA; lane 2, no RNA added to the cell-free system, and immunoprecipitated with hamster anti-T serum; lane [r] ...
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Poliovirus mutant that contains a cold-sensitive defect in viral RNA synthesis.
... To determine whether the 3A-2 defect selectively affected the synthesis of plus- or minus-strand RNA, cytoplasmic RNA was isolated from Vero cells that had been infected with wild-type o[r] ...
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Nuclear RNA Export and Packaging Functions of HIV-1 Rev Revisited
... HIV-1 RNA enhances the encapsidation of the genomic RNA to a much larger extent than the cytoplasmic RNA level indicates that the packaging function of Rev can be replaced by a het- erologous ...
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Analysis of Parvovirus mRNA by Sedimentation and Electrophoresis in Aqueous and Nonaqueous Solution
... This report describes a comparative analysis by sedimentation and electrophoresis in both aqueous and nonaqueous denaturing solvents of the stable cytoplasmic RNA transcribed from the ge[r] ...
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Autoregulation of Adenovirus Type 5 Early Gene Expression II. Effect of Temperature-Sensitive Early Mutations on Virus RNA Accumulation
... The fact that the differences in abundance of early RNA in the nuclei of cells infected by the mutants paralleled differences in cytoplasmic RNA abundance indicates that transport of nuc[r] ...
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Characterization of visna virus mRNA.
... We found that, during the acute infection in vitro, i viral mRNA's amount to only 0.1% of the total cytoplasmic RNA, ii 20% of the total cytoplasmic viral RNA is found in polyribosomes, [r] ...
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The Signal Peptide of a Simple Retrovirus Envelope Functions as a Posttranscriptional Regulator of Viral Gene Expression
... HIV RNA (57). In order to test whether JSE-SP facilitates cytoplasmic accumulation of unspliced viral RNA, we performed qRT-PCR on the nuclear and cytoplasmic RNA fractions of cells ...
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Long noncoding RNAs in renal diseases
... Brain cytoplasmic RNA 1; BC200: 200 nt brain cytoplasmic RNA; CASC2: Cancer susceptibility candidate 2; ccRCC: Clear cell renal cell carcinoma; ceRNA: Competing endogenous RNA; DN: ...
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Epstein-Barr virus RNA. VIII. Viral RNA in permissively infected B95-8 cells.
... With the possible exception of the 2.8-kb RNA encoded by EcoRIDhet, which seems to be a late RNA, viral cytoplasmic polyadenylated RNAs previously identified in cells latently infected w[r] ...
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RNA synthesis, movement and cytoplasmic microtubules in the teletrophic ovary of notonecta glauca glauca
... is RNA Is ribosom al (Brown, I 964 ; Brown & Dawld, I 968 ...al RNA i s achieved by a s e le c tiv e a m p lific a tio n of the rib o somal or n u c le o la r ...
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Distinct functions and requirements for the Cys-His boxes of the human immunodeficiency virus type 1 nucleocapsid protein during RNA encapsidation and replication.
... analysis. Cytoplasmic (1/10 plate equivalent) or virion-associated (1/3 plate equivalent) protein was suspended in protein loading buffer (10 mM Tris [pH ...
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Virus-Specific Ribonucleic Acid in the Nucleus and Cytoplasm of Rat Embryo Cells Transformed by Adenovirus Type 2
... Similar viral RNA has been observed in the nucleus of Ad 2RNA inhibited hybrid formation between labeled transformed rat embryo cells Sekikawa et al., cytoplasmic 3H-RNA and viral DNA ov[r] ...
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Structures of two spliced herpes simplex virus type 1 immediate-early mRNA's which map at the junctions of the unique and reiterated regions of the virus DNA S component.
... Cytoplasmic IE RNA was hybridized to 260-b fragment resulted from hybridization of either a 3', 32P-labeled SalB5 or a 5', 32P-labeled B4RJ HSV-1 DNA probe, and RNA/DNA hybrids the B4R1 [r] ...
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In Vitro Replication of Cowpea Mosaic Virus RNA I. Isolation and Properties of the Membrane-Bound Replicase
... DISCUSSION Our results show that CPMV-infected cowpea leaves contain an RNA-dependent RNA polymerase activity which appears to be closely bound with cytoplasmic membranes, and which is a[r] ...
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Nascent human immunodeficiency virus type 1 reverse transcription occurs within an enveloped particle.
... filtered cytoplasmic extract was found to be associated with a particle having a buoyant density similar to an intact virion, namely ...cell cytoplasmic extract were all shown to sediment higher in the ...
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