Defective-interfering (DI) RNAs
Conserved Motifs in a Tombusvirus Polymerase Modulate Genome Replication, Subgenomic Transcription, and Amplification of Defective Interfering RNAs
... ABSTRACT The replication of plus-strand RNA virus genomes is mediated by virally encoded RNA-dependent RNA polymerases (RdRps). We have investigated the role of the C-proximal region in the RdRp of tomato bushy stunt ...
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Assembled coronavirus from complementation of two defective interfering RNAs.
... virion RNAs demonstrated that coinfection of the pol/N sup- plier and the JW2 env supplier, but not other env suppliers, produced virus particles containing DI ...virion RNAs were produced in certain ...
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Common and distinct regions of defective-interfering RNAs of Sindbis virus.
... These 3' and 5' conserved sequences were the only regions of nucleotide sequence homology we detected between the Sindbis virus-derived DI RNA and the DI RNAs derived from Semliki Forest[r] ...
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Nonhomologous RNA recombination in tombusviruses: generation and evolution of defective interfering RNAs by stepwise deletions.
... The coordinates given correspond to those of the gRNA of TBSV (10) and represent the junction sites for all of the DI RNAs shown. The four regions which are conserved, to some degree, in[r] ...
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Broad-Spectrum Protection against Tombusviruses Elicited by Defective Interfering RNAs in Transgenic Plants
... DI RNAs have consid- erable potential for broad-spectrum control of the lethal dis- ease syndrome elicited by TBSV and several other members of the tombusvirus ...DI RNAs as sources of disease resistance? ...
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Translation but not the encoded sequence is essential for the efficient propagation of the defective interfering RNAs of the coronavirus mouse hepatitis virus.
... The defective interfering (DI) RNA MIDI of mouse hepatitis virus strain A59 (MHV-A59) contains a large open reading frame (ORF) spanning almost its entire ...DI RNAs containing different ...DI ...
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Efficient Homologous RNA Recombination and Requirement for an Open Reading Frame during Replication of Equine Arteritis Virus Defective Interfering RNAs
... natural defective interfering (DI) RNA of ...DI RNAs require the presence of an ORF for their efficient propagation, we have analyzed the importance of the EDI-ORF in EDI RNA ...
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A domain at the 3' end of the polymerase gene is essential for encapsidation of coronavirus defective interfering RNAs.
... These results strongly suggest that the packaging signal is located on an 800-nt Hindlll-EcoRI fragment containing 650 nt derived from the 3' end of ORFlb and 150 nt from the nucleocapsi[r] ...
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Effects of 5'-terminal modifications on the biological activity of defective interfering RNAs of Sindbis virus.
... RNA transcripts were transfected into chicken embryo fibroblasts in the presence of helper Sindbis virus, and the viral RNAs synthesized during the formation of passage 3 were analyzed b[r] ...
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Studies of defective interfering RNAs of Sindbis virus with and without tRNAAsp sequences at their 5' termini.
... RNAs from two independently isolated defective interfering particles of Sindbis virus contain a cellular tRNA sequence at their 5' ends. Common and distinct regions of defective interfer[r] ...
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Evolution of virus and defective-interfering RNAs in BHK cells persistently infected with Sindbis virus.
... tured RNAs obtained from BHK cells persistently infected with Sindbis virus for 16 months: polyadeny- lic acid-selected RNAs from wild-type Sindbis virus- infected BHK cells (lane 1) and[r] ...
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Analysis of efficiently packaged defective interfering RNAs of murine coronavirus: localization of a possible RNA-packaging signal.
... Domain I and most of the domain II of DIssF are also present in DIssE, and the junction between these two domains is identical to that of DIssE (18); however, DIssF contains 77 more nucl[r] ...
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Enhanced competitiveness of tomato bushy stunt virus defective interfering RNAs by segment duplication or nucleotide insertion.
... In these passage series, transcripts of the genome of cucumber necrosis tombusvirus (CNV [19], which shows 64% nucleotide identity to TBSV [7]) were used to differentiate between input T[r] ...
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Characterization of virulent and avirulent A/chicken/Pennsylvania/83 influenza A viruses: potential role of defective interfering RNAs in nature.
... The results suggest that the original avirulent virus was probably derived from influenza viruses from wild birds and that the virulent strain was derived from the avirulent strain by se[r] ...
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Structure of the intracellular defective viral RNAs of defective interfering particles of mouse hepatitis virus.
... Previously it was shown that the DI particles obtained from the serial undiluted passages of MHV-JHM contain an RNA species slightly smaller than the genomic RNA of standard virus (18)..[r] ...
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Evolution of defective-interfering double-stranded RNAs of the yeast killer virus.
... ScV-S RNAs are de- rived from M by internal deletion, sometimes followed by tandem duplication (2, 7), and they diverge in sequence from their parental RNA.. (2), as do the RNAs of anima[r] ...
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In vitro synthesis of large RNAs by an unusual defective interfering particle of vesicular stomatitis virus.
... Two serotype New Jersey DI particles, NJ-121 and NJ-PG2, synthesize both DI leader and large RNA transcripts in vitro (17); however, it is not known whether their synthesis is due to pol[r] ...
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In vitro replication of Sendai virus wild-type and defective interfering particle genome RNAs.
... The cell extracts for all subsequent experiments were therefore prepared from BHK cells infected with WT Sendai virus or coinfected with WT Sendai virus plus DI-H in the presence of acti[r] ...
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Conserved regions in defective interfering viral double-stranded RNAs from a yeast virus.
... This 11-bp sequence is not present in the 5' 1 kilobase of the parental RNA or in any of the sequenced regions of unrelated yeast viral dsRNAs, but it is present in the 3' region of the [r] ...
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Defective Interfering Influenza Virus RNAs: Time To Reevaluate Their Clinical Potential as Broad-Spectrum Antivirals?
... Defective interfering (DI) RNAs are highly deleted forms of the infectious genome that are made by most families of RNA vi- ...DI RNAs retain replication and packaging signals, are synthesized ...
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