The Escherichia coli long-‐term experiment
Large Chromosomal Rearrangements during a Long Term Evolution Experiment with Escherichia coli
... borders of the rearrangements with single-nucleotide resolu- tion, which is impossible with optical mapping data alone. We also verified the rearrangement borders for two evolved clones from populations Ara⫹1 and Ara⫹2, ...
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Changes in Intrinsic Antibiotic Susceptibility during a Long Term Evolution Experiment with Escherichia coli
... b Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA ABSTRACT High-level resistance often evolves when populations of bacteria are ex- posed to antibiotics, by ...
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Adaptation, Clonal Interference, and Frequency-Dependent Interactions in a Long-Term Evolution Experiment with Escherichia coli
... ABSTRACT Twelve replicate populations of Escherichia coli have been evolving in the laboratory for .25 years and 60,000 gener- ations. We analyzed bacteria from whole-population samples frozen every 500 ...
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Fixation and Adaptation in the Lenski E. coli Long Term Evolution Experiment
... this experiment can be found at the following link ...mega-plate experiment demonstrates that adap- tation can occur without fixation ...mega-plate experiment demonstrates the difference between ...
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Genomewide Dam Methylation in Escherichia coli during Long-Term Stationary Phase
... In Escherichia coli K-12 and many gammaproteobacteria, this modification is catalyzed by DNA adenine methyltransferase (Dam) at the GATC consensus sequence and is known to modulate cellular processes ...
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Long-Term Experimental Evolution in Escherichia coli. VI. Environmental Constraints on Adaptation and Divergence
... In contrast with replicate populations selected in glucose-limited medium, the mean fitness of populations selected in maltose me- dium was similar in both selected [r] ...
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Long-Term Experimental Evolution in Escherichia coli. IV. Targets of Selection and the Specificity of Adaptation
... yet the pathways for degradation of these two nutrients are quite similar (Figure 3). 1983) is quite small, and, for the reasons stated above, these genes are un- likely to have[r] ...
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Parallel Changes in Global Protein Profiles During Long-Term Experimental Evolution in Escherichia coli
... Manuscript received August 15, 2005 Accepted for publication May 11, 2006 ABSTRACT Twelve populations of Escherichia coli evolved in and adapted to a glucose-limited environment from a common ancestor. We ...
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Long-Term Experimental Evolution in Escherichia coli. XII. DNA Topology as a Key Target of Selection
... of Escherichia coli, founded from a common ancestor and serially propagated for 20,000 generations, during which time they achieved substantial fitness ...
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Adaptation of Escherichia coli to Long-Term Serial Passage in Complex Medium: Evidence of Parallel Evolution
... Genomic population dynamics during long-term stationary phase. In order to determine what mutations in each population, and at each passage, may lead to the adaptive phenotypes, we performed whole-genome ...
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Long-Term Experimental Evolution in Escherichia coli. IX. Characterization of Insertion Sequence-Mediated Mutations and Rearrangements
... ABSTRACT As part of a long-term evolution experiment, two populations of Escherichia coli B adapted to a glucose minimal medium for 10,000 generations. In both populations, multiple ...
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Longitudinal genomic surveillance of multidrug-resistant Escherichia coli carriage in a long-term care facility in the United Kingdom
... coli reference genome NCTC13441 (European Nucleo- tide Archive [ENA] accession number ERS530440) using SMALT v0.7.4 [43]. Variants were detected using sam- tools mpileup v0.1.19 [44] and the parameters ‘-d 1000 – ...
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Functional Analysis of Antigen 43 in Uropathogenic Escherichia coli Reveals a Role in Long-Term Persistence in the Urinary Tract
... of the two Ag43 variants, ORF52 III , is expressed at higher levels than the other, ORF47 V (2). Two strategies were employed to determine the role of Ag43a and Ag43b in virulence in the mouse urinary tract. First, ...
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Multiple Host Sharing, Long Term Persistence, and Virulence of Escherichia coli Clones from Human and Animal Household Members
... James R. Johnson,* Connie Clabots, and Michael A. Kuskowski Veterans Affairs Medical Center and Departments of Medicine and Psychiatry, University of Minnesota, Minneapolis, Minnesota Received 21 May 2008/Returned for ...
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Zoonotic Escherichia coli
... E. coli O157:H7 was demon- strated to persist for several months. Long-term survival has also been observed in fresh ma- nure, while a rapid decrease seems to occur in ...
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CHARACTERISTICS OF ESCHERICHIA COLI
... meningitis, encephalitis, and intrauterine or cervical infections in pregnant women, which may result in spontaneous abortions during the second or third trimester, or in stillbirths. Pregnant women usually suffer ...
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CLONING IN ESCHERICHIA COLI
... E. coli cells provides a combined selection (for the ampicillin resistance phenotype) and screen (for the interrupted lacZ gene) for cells carrying the recombinant ...the experiment require the isolation of ...
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Nitrogen efficiency of spring barley in long term experiment
... that after using of 80 kg N/ha average nitrogen content in grain increased about 18% compared to unfertilized treatment. Average values of NUtE, RE N and AE N can be found in Table 3. Higher efficiency of nitrogen from ...
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Parallel evolution of HIV-1 in a long-term experiment
... phylogenetic analyses when the evolutionary history of sampled populations is not known. The extent of parallel evolution in our experiment is likely to be determined by the number of high fitness effect mutations ...
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Evaluation of Acquired Antibiotic Resistance in Escherichia coli Exposed to Long Term Low Shear Modeled Microgravity and Background Antibiotic Exposure
... The long-term response of microbial communities to the microgravity environment of space is not yet fully ...study, Escherichia coli cells were grown under low-shear modeled microgravity ...
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