Gene isolation and sequence analysis
Isolation, Cloning, and Sequence Analysis of the Full-Length RFT1 Gene from Malaysian Upland Rice (Oryza sativa, subsp.
... Conclusion Isolation of the qualified RNA and full-length Mu- RFT1 gene were performed in the current ...The gene was subsequently constructed into the cloning vector pGEM-T and transformed into the ...
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Isolation, amplification and sequence analysis of the gene encoding Enolase from lymphatic filarial parasite Brugia malayi
... ABSTRACT Lymphatic filariasis, caused by Brugia malayi, commonly known as elephantiasis, is a neglected tropical disease. No vaccines are available for the prevention of filarial infections. A number of pathogenic ...
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Isolation, sequence identification and tissue expression profile of a novel goat gene-RAP1B
... cDNA sequence of one goat gene, RAP1B, was amplified using the rapid amplification of cDNA ends (RACE) method based on one goat EST sequence which was highly homologous to the coding sequence ...
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The isolation and sequence of missense and nonsense mutations in the cloned bacteriophage P22 tailspike protein gene.
... BARAM 1989), and from the plasmid clone of the tailspike protein gene has been undertaken. This analysis is possible because of the ability to comple- ment absol[r] ...
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Isolation, cloning and sequence analysis of lactate dehydrogenase gene from Theileria annulata may lead to design of new antitheilerial drugs
... The isolation, cloning and analysis of lactate dehydrogenase from ...dehydrogenase sequence was isolated from from a Theileria ...the sequence was cloned into the vector pGEM-T ...whole ...
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Microbial diversity analysis, sequence isolation and phylogenetic analysis of south indian gundaru river basin
... rRNA gene analysis provides accurate identification at the species level and can clarify their clinical ...rRNA gene sequence to elevate both unusual aerobic gram negative bacilli and ...
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The isolation and characterisation of the novel gene C53
... using sequence data to pre-screen the endoderm library prior to expression analysis by ...to sequence databases using the NCBI BlastN program, which compares nucleotide sequences to the database, and ...
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Isolation of a Papain like Cysteine Proteases (PLCP) Gene Sequence from the Genomic DNA of Local Carica Papaya Plant
... DNA Isolation The fresh leaves of the Carica papaya were used for Genomic DNA ...The isolation of the nucleic acid (avoiding RNA by use of RNases) was according to the protocols defined by Doyle and Doyle ...
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Identification of Mycobacterial Species by Comparative Sequence Analysis of the RNA Polymerase Gene (rpoB)
... The genus Mycobacterium comprises a wide range of organ- isms, including obligate parasites causing serious human and animal diseases, opportunistic pathogens, and saprophytic spe- cies found in nature. Human infections ...
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Isolation and expression analysis of stimulator of interferon gene from olive flounder, Paralichthys olivaceus
... acid-inducible gene I (RIG-I) and TANK-binding kinase 1 (TBK1) by initiating IFN expression, which might facilitate the immune responses against viral attack (Sun et ...1 Sequence identities and ...
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Sequence Analysis of the ank Gene of Granulocytic Ehrlichiae
... Although analysis of the 16S rRNA gene sequence is a pow- erful tool for identifying novel agents and for determining relatedness at the genus and species level, the strong conser- vation of the 16S ...
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Isolation and Analysis of the arg-13 Gene of Neurospora crassa
... Several lines of biochemical evidence have suggested the presence of a basic amino acid transport function in the mitochondrial membrane, a barrier that acts to [r] ...
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Phylogeny and Identification of Enterococci by atpA Gene Sequence Analysis
... AFLP analysis, SDS-PAGE of whole-cell proteins, and, if they were available, other polyphasic data and represent the known heterogeneity of Enterococcus ...atpA gene sequence similarity; the ...atpA ...
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Sequence Analysis of the msp4 Gene of Anaplasma phagocytophilum Strains
... 4 gene (msp4) was identified, and the coding region was PCR amplified and sequenced from a variety of sources, including 50 samples from the United States, Germany, Poland, Norway, Italy, and Switzerland and 4 ...
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The chicken c-fos gene: cloning and nucleotide sequence analysis.
... The 5'-noncoding region which is highly homologous to that of the mouse c-fos gene and the A +T-rich stretch of 67 bp (12) are indicated by lightface underlining, and both regions are co[r] ...
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Sequence and Phylogenetic Analysis of SSU rRNA Gene of Five Microsporidia
... rRNA) gene sequences of five microsporidia including Nosema heliothidis, and four novel microsporidia isolated from Pieris rapae, Phyllobrotica armta, Hemerophila atrilineata, and Bombyx mori, respectively, were ...
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Mutation analysis of the coding sequence of the MECP2 gene in infantile autism
... The mutation analysis of the coding sequence of the gene was performed by denaturing high-pressure liquid chro- matography and direct sequencing. Taken together, 14 se- quence variants were ...
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Sequence and transcription analysis of the human cytomegalovirus DNA polymerase gene.
... The HCMV pol gene is flanked by open reading frames with homology to those of other herpesviruses; upstream, there is a reading frame homologous to the glycoprotein B gene of herpes simp[r] ...
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Gene-Based Sequence Diversity Analysis of Field Pea (Pisum)
... ABSTRACT Sequence diversity of 39 dispersed gene loci was analyzed in 48 diverse individuals representative of the genus ...Thus, gene regions and intergenic ‘‘junk DNA’’ share a consistent picture ...
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SEQUENCE AND POLYMORPHISM ANALYSIS OF THE CAMEL (CAMELUS DROMEDARIUS) MYOSTATIN GENE
... the sequence variability at the Camelus dromedarius MSTN locus was still ...extensive sequence and polymorphism analysis of the MSTN gene in the ...nucleotide sequence screened on 22 ...
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