High Resolution Light Microscopy
Light sheet theta microscopy for rapid high-resolution imaging of large biological samples
... the light sheet thickness), and a second iris is placed at the conjugate plane between the scan lens and tube lens to control the light sheet width ...of light sheet perpendicular to its propagation ...
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Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy
... New light microscopy techniques are constantly becoming available and are being applied to the study of nuclear ...example, light-sheet microscopy techniques use orthogonal illumination to ...
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High Resolution Imaging and Digital Characterization of Skin Pathology By Scanning Acoustic Microscopy
... the light source. Therefore, the resolution of OCT system is determined by the coherence length and the power of the light source used, the focal spot size of the lens and the step width[45], ...
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Investigating CNS synaptogenesis at single synapse resolution by combining reverse genetics with correlative light and electron microscopy
... (E-H) High-magnification images of LifeAct::GFP and Actin::GFP at the first branch point (E,G) and in the midline region of the anterior commissure ...) High-magnification images of the boxed regions in ...
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Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins
... In this work, we described the application of SMLM on cryosamples to increase the interpretability of cryo- CLEM, and addressed several challenges. Firstly, the increased drift of cryostages had to be mitigated. We have ...
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Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy
... spatial resolution of an imaging system is defined as the capacity to distinguish closely separated features; in light mi- croscopy, this is limited by diffraction to ∼ 200 − 300 ...Consequently, ...
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Atomic force microscopy: a powerful tool for high-resolution imaging of spermatozoa
... of light to scan the samples with optical tips, which can be applied in the tapping or contact ...A resolution of few tens of nanometers is achievable by SNOM. High resolution top- ographic ...
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Selective plane illumination microscopy techniques in developmental biology
... the light-sheet and a loss in ...the light- sheet as it penetrates dense tissue. The thickness of the light-sheet (and of the optical section) might increase with depth from ~5 μ m to 10-15 μ m in a ...
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Resolution Enhancement in Phase Microscopy: a Review
... phase microscopy [23,26] generates a reference beam by pinhole- filtering on one copy of the object ...and high lateral and phase ...Interferometric Microscopy) proposes a simple and low-cost way to ...
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Light sheet fluorescence microscopy for neuroscience
... wide light exposure combined with the limited acquisition speed results in a much higher exposure of the sam- ple causing potential ...Moreover, high resolution imaging deep into biological tissues ...
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Applying compressive sensing to TEM video: a substantial frame rate increase on any camera
... electron microscopy (TEM) has established itself as a very powerful analytical technique for its ability to provide a direct insight into the nature of materials under a broad range of environmental condi- ...
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Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?
... tissue optically transparent. Compared with traditional tech- niques, this has the advantage of leaving complex intercellu- lar pathways intact and retaining dispersed, subtle features [1–3]. This allows us not only to ...
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Local surface structure and composition control the hydrogen evolution reaction on iron nickel sulfides
... Abstract: In order to design more powerful electrocatalysts, develo- ping understanding of the role of surface atomic-structural composi- tion of widely abundant bulk materials is crucial. This is particularly true in ...
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Transplant biopsy beyond light microscopy
... The results obtained from genome-wide expression studies in kidney transplant biopsies indicate that the vast majority of differentially expressed molecules rep- resent a stereotyped tissue response to injury caused by ...
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Cai, Ruiyao (2019): DISCO whole body clearing and imaging to study systemic changes in neuronal pathologies. Dissertation, LMU München: Graduate School of Systemic Neurosciences (GSN)
... Besides the activation of resident microglia, an infiltration of peripheral macrophages attracted by chemokines into the CNS after SCI has been observed 130-132 . These infiltrating cells are actively involved in the ...
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CONCENTRATION IMPACT AND STRUCTURE SUITABILITY OF ACETYL SALICYLATE MOEITY (ASPIRIN) AS MULTI-FUNCTIONALIZED DRUG
... The present investigations discuss the correlation between concentrations of active acetyl salicylate moiety in the synthesized tablet and solvation rates in different pH-media. Furthermore AFM-microscopy was to ...
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Phase pure iron pyrite nanocrystals for low cost photodetectors
... The high-magnification images in Figure 2b,c,d,e,f,g show that as [S]/[Fe] increases, the average size of the nanoparticles increases in addition to the morphology ...
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Synthesis and Characterization of CdS, ZnS and CdZnS Nanoparticles Embedded in Polystyrene
... The room temperature UV-Vis absorption spectrum of the ZnS composite is shown in Figure 5. The extrapo- lation of the straight line region of the plot of the absorbance against wavelength to zero shows that the absorp- ...
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Correlated Cryo Super‐Resolution Light and Cryo‐Electron Microscopy on Mammalian Cells Expressing the Fluorescent Protein rsEGFP2
... There are many “pause points” throughout the protocol that are noted in the Supporting Information which allow samples to be stored in liquid nitrogen (LN2) for extended periods of time. A complete list of materials and ...
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Articles
... a high-resolution confocal microscopy; b) measurements of the disc cypsela with a stereoscopic microscope – duly scaled; c) its distribution in several bioclimatic belts; d) field observations; e) ...
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