Human embryonic germ cells
Differentiation of Human Embryonic Stem Cells into Embryoid Bodies Comprising the Three Embryonic Germ Layers
... ing cells within the EBs we examined by in situ hybridization the expression of four cell- specific molecular markers, all of which were transcribed very early during embryonic dif- ...from human ...
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Supplementation of vitamin C promotes early germ cell specification from human embryonic stem cells
... early germ line may be mediated by Tet enzymes, which convert 5mC to 5-hydroxy-methylcytosine (5hmC) [18, ...malian cells, including embryonic stem cells and mouse embryonic fibroblasts ...
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Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns
... non- human primate and human ESCs to diff erentiate into germ cell lineages, specifi cally in vitro-derived PGCs (invPGCs) ...into germ cells by forming embryoid bodies (EBs) with ...
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STELLA Facilitates Differentiation of Germ Cell and Endodermal Lineages of Human Embryonic Stem Cells
... developing germ cells, where it localizes in both nucleus and cytoplasm ...Stella-negative cells [2,3]. This finding implies that PGCs have undergone the germ cell specification program with a ...
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Retinoic acid improve germ cell differentiation from human embryonic stem cells
... stem cells (SSCs). These cells are able to undergo meiosis, generate haploid male gametes, and may fertilize after intracytoplasmic injection into mouse ...oocyte-like cells. PGCs were differentiated ...
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A germ cell origin of embryonic stem cells?
... pluripotent cells of the intact early embryo proliferate for only a limited period of time, it was not initially obvious whether pluripotent cell lines could be established without undergoing malignant ...EC ...
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A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions
... labeled cells formed multiple organs in chimeric mice suggesting that, when placed in the proper conditions, the cells could differentiate into multiple different lineages ...stem cells could ...
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EXAMINATION OF THE GERM CELL CHIMERA FORMING POTENTIAL OF MOUSE EMBRYONIC STEM CELLS
... To get 2-cell stage embryos, females were superovulated with intraperitoneal injection of pregnant mare’s serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG) at specific times (1). After hCG ...
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VSELs may obviate cryobanking of gonadal tissue in cancer patients for fertility preservation
... in human cortical biopsies lead to primordial follicle assembly ...OSE cells smears showed the presence of small (2–5μm), round cells which expressed pluripotent markers SSEA- 4, Oct-4, Nanog, Sox-2, ...
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Impaired meiotic competence in putative primordial germ cells produced from mouse embryonic stem cells
... some cells able to enter meiosis were undetected in our assay or will enter meiosis after longer period in culture or if transferred into a favourable ...and human ESCs can give rise to haploid germ ...
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Current protocols in the generation of pluripotent stem cells: theoretical, methodological and clinical considerations
... stem cells have been derived from various embryonic, fetal and adult ...sources. Embryonic stem cells (ESCs) and parthenogenic ESCs (pESCs) are derived from the embryo proper while ...
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Targeted DamID reveals differential binding of mammalian pluripotency factors
... homogeneous cells and antisera with high ...fewer cells than ChIP-based ...mouse embryonic stem cells, epiblast-like cells and primordial germ cell-like cells ...
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Transgenerational epigenetics in the germline cycle of Caenorhabditis elegans
... drive germ cell specification at each ...in germ cells and soma, as perhaps there should be given the germline’s connectivity of generations that span millennia, versus the soma’s single generation ...
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On the development of extragonadal and gonadal human germ cells
... The sex genotyping of the human fetal tissues, processing for histology and immunofluorescence were performed essentially as previous described . All immunofluorescence staining was performed using 0.01 M citric ...
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Genetic Requirement For The Rna Helicase Mov10l1 In Pirna Biogenesis
... The most well-characterized targets of piRNAs are transposable elements (TEs). The depletion of piRNAs in mice, Drosophila, and zebrafish resulted in a massive de- repression of TEs (Aravin et al., 2007; Brennecke et ...
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The control of sexual identity in the Drosophila germline
... with germ cell- autonomous cues to control proper germ cell sexual development is also crucial for understanding human germ cell development and ...fertility. Human disorders such as ...
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Stem cell technology for bone regeneration: current status and potential applications
... fat are superior to BMSC and dental pulp mesenchymal stem cells (DMSC), as they can be procured in abundance with minimal patient morbidity. Further, as the PSC are puri- fied via FACS instead of being cultured, ...
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Exploring The Role Of Tet1 In Genomic Imprinting
... Our lab’s experiments and other recent studies employing knockdown and knockout models have explored whether the TET enzymes may play a role in the two waves of DNA methylation that occur during development. These ...
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Defining stemness of human embryonic stem cells: a systems biology approach
... murine embryonic stem cell clones ...no human version has been ...technique. Embryonic lethality, affecting about a third of the knockouts generated, is the most prominent problem which prevents the ...
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piRNAs and PIWI proteins: regulators of gene expression in development and stem cells
... the germ plasm – a specialized cytoplasm required for germ cell ...the germ plasm ...to embryonic patterning (Rouget et ...in embryonic development through the regulation of essential ...
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