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Insertion site

Insertion site preference of Mu, Tn5, and Tn7 transposons

Insertion site preference of Mu, Tn5, and Tn7 transposons

... random insertion profile than either Mu or ...poson insertion site preference is ...Mu insertion have shown that parti- cular dinucleotides, or base steps, contribute to site pre- ...

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Retrovirus insertion site analysis of LGL leukemia patient genomes

Retrovirus insertion site analysis of LGL leukemia patient genomes

... the insertion call pipeline is given in Additional file 1: Supplementary ...retroviral insertion result in aberrant mapping of long insert mate pairs to the reference ...candidate insertion events. ...

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Reverse Dot Blot Assay (Insertion Site Typing) for Precise Detection of Sites of IS6110 Insertion in the Mycobacterium tuberculosis Genome

Reverse Dot Blot Assay (Insertion Site Typing) for Precise Detection of Sites of IS6110 Insertion in the Mycobacterium tuberculosis Genome

... E7 insertion being identified for one of the ...four insertion sites in strain 1551, strain 28 contained only the A1 insertion site, which is typically found in Beijing family strains ...of ...

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Chromosomal Distribution of Transposable Elements in Drosophila melanogaster Test of the Ectopic Recombination Model for Maintenance of Insertion Site Number

Chromosomal Distribution of Transposable Elements in Drosophila melanogaster Test of the Ectopic Recombination Model for Maintenance of Insertion Site Number

... The insertion site number weighted by DNA content was not correlated with recombination rate for all TEs except hobo, for which a positive correlation was detected.. No g[r] ...

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Characterization of a Permissive Epitope Insertion Site in Adenovirus Hexon

Characterization of a Permissive Epitope Insertion Site in Adenovirus Hexon

... Effect of peptide insertion on infection kinetics and virus yield. After adenovirus enters the cell and traffics to the nu- clear envelope via microtubules (25, 30), it is necessary for the viral DNA to enter the ...

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Prospective multicenter study of vascular catheter related complications and risk factors for positive central catheter cultures in intensive care unit patients

Prospective multicenter study of vascular catheter related complications and risk factors for positive central catheter cultures in intensive care unit patients

... In contrast, the purpose of the cannula nutrition and monitoring of central venous pressure, the insertion site jugular, the dressing type semipermeable transparent dressing, the antisep[r] ...

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Sonological Evaluation of gestational Age by Placental Thickness

Sonological Evaluation of gestational Age by Placental Thickness

... Many fetal parameters are used sonographically to date pregnancy which includes fetal bi-parietal diameter (BPD), head circumference (HC), femur length (FL) and abdominal circumference (AC) and along with that placental ...

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Phenotypic and Genotypic Characterization of EnteropathogenicEscherichia coli (EPEC) strains in Tehran, Iran

Phenotypic and Genotypic Characterization of EnteropathogenicEscherichia coli (EPEC) strains in Tehran, Iran

... The insertion sites of LEE in serogrouped isolates were selC (in 6 isolates), pheU (in 7 isolates) and pheV (in 2 ...The insertion site in 2 isolates was not determined by ...

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Estimation of Placental Thickness by Ultrasonography and its Correlation with Gestational Age in Normal and Intrauterine Growth Retardation Pregnancies in Late 2 nd And 3 rd Trimester

Estimation of Placental Thickness by Ultrasonography and its Correlation with Gestational Age in Normal and Intrauterine Growth Retardation Pregnancies in Late 2 nd And 3 rd Trimester

... Material and Methods: Using Ultrasonography placental thickness was measured at cord insertion site from 18 th to 40 th gestational age by LMP in pregnant woman. At term cases were categorized as normal and ...

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Characterization of the flamenco Region of the Drosophila melanogaster Genome

Characterization of the flamenco Region of the Drosophila melanogaster Genome

... P-element insertion point, might be a good candidate for a gypsy regulator, since it putatively encodes a nuclear protein containing two double- stranded RNA-binding ...

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Targeted identification of TE insertions in a Drosophila genome through hemi-specific PCR

Targeted identification of TE insertions in a Drosophila genome through hemi-specific PCR

... assays. Insertion site assays combined forward and reverse primers on either side of each insertion site, such that potential PCR products would include both the reference and the ...

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The relation between atrial fibrillation wavefront characteristics and accessory pathway conduction

The relation between atrial fibrillation wavefront characteristics and accessory pathway conduction

... However, it could be approximated either by the time of atrial wavefront arrival at the accessory pathway atrial insertion site, or by the time of earliest ventricular activation at the [r] ...

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Maize Mu Transposons Are Targeted to the 5′ Untranslated Region of the gl8 Gene and Sequences Flanking Mu Target-Site Duplications Exhibit Nonrandom Nucleotide Composition Throughout the Genome

Maize Mu Transposons Are Targeted to the 5′ Untranslated Region of the gl8 Gene and Sequences Flanking Mu Target-Site Duplications Exhibit Nonrandom Nucleotide Composition Throughout the Genome

... Mu insertion sites: To identify the CCAAT boxes in the 5⬘ region of the gl8 ...Mu insertion site for each of the 79 gl8-Mu alleles, PCR Isolation of gl8-Mu alleles: The gl8 gene product is was ...

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New Trypanosoma cruzi Repeated Element That Shows Site Specificity for Insertion

New Trypanosoma cruzi Repeated Element That Shows Site Specificity for Insertion

... Genomic organization of the TcTREZO element. When a Southern blot of T. cruzi genomic DNA digested with several restriction enzymes was hybridized with TcTREZO, a very simple pattern of hybridization was observed (Fig. ...

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Mapping of IS6110 Insertion Sites in Two Epidemic Strains  of Mycobacterium tuberculosis

Mapping of IS6110 Insertion Sites in Two Epidemic Strains of Mycobacterium tuberculosis

... IS6110 insertion sites in strain W. Our hypothesis is that an IS6110 insertion site(s) could possibly be responsible for a strain’s increased capacity for transmission and/or ...the insertion ...

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Insertional Gene Activation by Lentiviral and Gammaretroviral Vectors

Insertional Gene Activation by Lentiviral and Gammaretroviral Vectors

... Insertion site identification. For inverse PCR (5), genomic DNA was digested with NlaIII (HV vector) or MspI (HV/CNCG/MFG vectors) and then ligated. Nested PCR on circularized DNA was performed by using ...

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Site-directed and linker insertion mutagenesis of herpes simplex virus type 1 glycoprotein H.

Site-directed and linker insertion mutagenesis of herpes simplex virus type 1 glycoprotein H.

... Linker insertion mutagenesis. gH linker insertion mutants were generated according to protocols described by Digard et ...the insertion site, the oligonucleo- tide was designed to contain a ...

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Determining the Genomic Locations of Repetitive DNA Sequences with a Whole Genome Microarray: IS6110 in Mycobacterium tuberculosis

Determining the Genomic Locations of Repetitive DNA Sequences with a Whole Genome Microarray: IS6110 in Mycobacterium tuberculosis

... The site was still regarded as confirmed because the products were in fact present and because some differences in genomic sequence between H37Rv and other isolates can be ...the insertion had been assigned ...

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DNA transposons have colonized the genome of the giant virus Pandoravirus salinus

DNA transposons have colonized the genome of the giant virus Pandoravirus salinus

... paralogous site that lacked the MITE, but contained a copy of the TA dinucleotide at the insertion site ...empty site; ...empty site is 11 % over 119 total bp; e-value = ...

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Mapping of the Saccharomyces cerevisiae Oxa1-Mitochondrial Ribosome Interface and Identification of MrpL40, a Ribosomal Protein in Close Proximity to Oxa1 and Critical for Oxidative Phosphorylation Complex Assembly

Mapping of the Saccharomyces cerevisiae Oxa1-Mitochondrial Ribosome Interface and Identification of MrpL40, a Ribosomal Protein in Close Proximity to Oxa1 and Critical for Oxidative Phosphorylation Complex Assembly

... exit site of the large ribosomal subunit (3, 10, 23, 27, ...the site of membrane insertion into the inner membrane, exists in close physical proximity to the large ribosomal subunit and specifi- ...

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