Inverse PCR
Isolation and Analysis of Retroviral Integration Targets by Solo Long Terminal Repeat Inverse PCR
... that inverse PCR taking advantage of the solo LTR allowed simultaneous cloning of the upstream and downstream flank- ing sequences in a single plasmid ...LTR inverse PCR (SLIP), could be used ...
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Rapid Detection of the Mycobacterium tuberculosis Beijing Genotype and Its Ancient and Modern Sublineages by IS6110 Based Inverse PCR
... instances PCR typing further FIG. 2. Examples of IS6110 inverse-PCR profiles of ...the PCR fragments specific for the Beijing genotypes (two- or three-band ...
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Heminested inverse PCR for IS6110 fingerprinting of Mycobacterium tuberculosis strains
... crude PCR product eliminated most of the diffusely staining (ethidium bromide) background material on the gels which was observed for strains with high copy numbers of IS6110 (data not ...the inverse ...
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An inverse PCR screen for the detection of P element insertions in cloned genomic intervals in Drosophila melanogaster.
... We prepared PCR-labeled probes from each of these inde- pendent lines using the method described above except that only primers corresponding to the P element in- verted rep[r] ...
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Characterization of Avirulent TnphoA Mutants in Agrobacterium tumefaciens to Enhance Transformation Efficiency
... Protein fusion with the Escherichia coli alkaline phosphatase is used extensively for the analysis of the topology of membrane protein. Agrobacterium strain A6007 was mutagenized with E. coli strain mm294A plasmid pRK609 ...
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Molecular analysis of a haemagglutinin of Haemophilus paragallinarum
... used inverse PCR as shown in ...The inverse PCR products from strain Modesto were used to produce the full-length contig containing the gene encoding the putative haemagglutinin, termed ...
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Mycoviruses as Triggers and Targets of RNA Silencing in White Mold Fungus Sclerotinia sclerotiorum
... (B) Inverse PCR product amplified using SsHADV-1-specific primers to confirm the infectivity of the infectious clone from a >6 times transferred ...
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Insertional mutagenesis of flvi-2 in tumors induced by infection with LC-FeLV, a myc-containing strain of feline leukemia virus.
... Thus, the technique of inverse PCR, in combination with data from Southern blot analysis, demonstrates the presence and orientation of FeLV proviral DNA integrated atflvi-2 in five of si[r] ...
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Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets
... conventional phage panning that include laborious colony picking and functional ligand screening. A downside of this approach is that the only output from such experiments is the DNA sequence information of such enriched ...
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Identification of piggyBac mediated insertions in Plasmodium berghei by next generation sequencing
... and inverse PCR, the half-nested PCRs were performed for all identified in- sertion ...nested PCR with a site-specific primer (Additional file 1) and a pair of primers located in either of the ...
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Deletion mutagenesis of large areas in Plasmodium falciparum genes: a comparative study
... used PCR-based mutagenesis methods identified a modified inverse PCR method as particularly suitable for the deletion of large areas (>100 bp) in malaria parasite ...
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Factorizable inverse monoids
... Factorizable inverse monoids constitute the algebraic theory of those partial symmetries which are restrictions of automorphisms; the formal de…nition is that each element is the product of an idempotent and an ...
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RFLP-PCR is more efficient than ARMS-PCR for identifying CYP2C19*2 polymorphism in atherosclerotic patients
... It has been shown that CYP2C19*2 polymorphisms are related to atherosclerosis in certain ethnic groups, including the Brazilian population. It has also been demonstrated that different polymorphism identification ...
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A STUDY AND COMPARATIVE ANALYSIS OF VARIOUS LANE MARKING TECHNIQUES
... RANSAC is a random sample consensus. It is used for estimation of parameters because it is an iterative method. It is a learning technique for estimation of parameters. Random sample consensus (RANSAC) can estimate one ...
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Inverse methods and modelling
... Because the space occupied by atoms is so small, the scattering is sufficiently weak most of the incident X-ray beam passes undeflected through the crystal that the Born approximation §l[r] ...
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Inverse Trigonometric Functions
... many functions which are not one-one, onto or both and hence we can not talk of their inverses. In Class XI, we studied that trigonometric functions are not one-one and onto over their natural domains and ranges and ...
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Inverse tone mapping
... Abbreviations • A: Aky¨uz et al.’s operator [7] • B: Banterle et al.’s operator Section 5.1 • B2 : method in Section 5.2 • BRDF: Bi-directional Reectance Distribution Function • BTC: Blo[r] ...
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The Discriminance for FLDcircr Matrices and the Fast Algorithm of Their Inverse and Generalized Inverse
... By constructing the basic FLDcircr matrix, we give the discriminance for FLDcircr matrices and the fast algorithm of the inverse and generalized inverse of the FLDcircr matrices... Keywo[r] ...
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INTERNATIONAL JOURNAL OF ENGINEERING SCIENCES & MANAGEMENT AUTHENTICATION WITH AES CRYPTOGRAPHIC ENCRYPTION CHIP DESIGN AND SIMULATION Nitin Gupta*, Manoj Kumar
... the inverse affine transform values have to find for inverse Subbytes transformation then multiplicative inverse of that ...bytes. Inverse Subbytes transformation is a method of inverse ...
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On the use of hot-spot stresses, effective notch stresses and the Point Method to estimate lifetime of inclined welds subjected to uniaxial fatigue loading
... ,∆ , fatigue constants for the MWCM’s calibration curves negative inverse slope of the uniaxial fatigue curve negative inverse slope of the torsional fatigue curve negative inverse slope[r] ...
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