ITS1-5.8S-ITS2 region
Genetic Variation among Fragmented Populations of Atriplex halimus L Using Start Codon Targeted (SCoT) and ITS1 5 8S ITS2 Region Markers
... populations. In the present study, the mean value of Nei’s gene diversity index (h) was 0.197, near to the mini- mum h value (0.174 - 0.328) of nine out-crossing plants summarized by Schoen and Brown [39]. The estimate ...
15
Multigene-Based Analyses of the Phylogenetic Evolution of Oligotrich Ciliates, with Consideration of the Internal Transcribed Spacer 2 Secondary Structure of Three Systematically Ambiguous Genera
... and ITS1-5.8S- ITS2 region sequence data (12, ...sequences, ITS1-5.8S-ITS2 region sequences, and ITS2 secondary structure) in order to infer evolutionary relationships ...
9
Identification to the Species Level and Differentiation between Strains of Aspergillus Clinical Isolates by Automated Repetitive Sequence Based PCR
... the ITS1-5.8S-ITS2 region of each Aspergillus isolate showed both single nucleotide differences and short lengths of sequence variation (data not ...the ITS1 and ITS2 regions was ...
9
Genotyping of Pneumocystis jirovecii by Use of a New Simplified Nomenclature System Based on the Internal Transcribed Spacer Regions and 5 8S rRNA Gene of the rRNA Operon
... the ITS1-5.8S-ITS2 genotypes of 27 ...jirovecii ITS1 and ITS2 se- quences currently available from GenBank, and evaluated the intrastrain variability of multiple homopolymeric tracts within ...
11
Molecular and Pathogenic Study of Guignardia spp Isolates Associated to Different Hosts
... Fungi of Guignardia genus are commonly isolated from different plant species and most of the time, they are characterized as endophytes. However, some species of this genus, like G. citricarpa and G. psidii are known as ...
10
Update on Pneumocystis carinii f sp hominis Typing Based on Nucleotide Sequence Variations in Internal Transcribed Spacer Regions of rRNA Genes
... ITS region I (ITS1) is located between the 18S and ...and ITS2 is located between the 5.8S and 26S rRNA genes. ITS1 was found to contain 157 bp, and ITS2 was deter- mined to be 177 bp ...
8
Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5 8S Ribosomal DNA Typing in Ocular Infections
... and ITS2 and ...this region were carried out to evaluate the sensitivity and specificity of the ...the ITS2/5.8S region of all the fungal strains by this PCR ...
7
Multilocus DNA Sequence Comparisons Rapidly Identify Pathogenic Molds
... and ITS1/ITS2 DNA sequence-based phylogenetic trees of clinically significant ...complete ITS1, ...hypervariable region of the 28S rRNA ...
10
The genetic variation of Angiostrongylus cantonensis in the People’s Republic of China
... Results: We characterised the complete mitochondrial genes cox1 and nad1 of 130 specimens and obtained 357 nuclear sequences containing two complete ITS (ITS1 and ITS2) and 5.8S rRNA of the same samples. ...
11
DNA Microarray Based on Arrayed Primer Extension Technique for Identification of Pathogenic Fungi Responsible for Invasive and Superficial Mycoses
... available ITS1 and/or ITS2 sequences (for accession numbers, see Table ...the region carrying ITS1, the 5.8S rRNA gene, and ITS2 and varied in size from 375 bp (Candida pulcherrima) to ...
7
Malassezia arunalokei sp nov , a Novel Yeast Species Isolated from Seborrheic Dermatitis Patients and Healthy Individuals from India
... the ITS2 region (273 bp), with 8 deletions and 16 ...and ITS2 regions), nontranscribed spacer regions (IGS regions) have been successfully used to identify fungal spe- cies and to discriminate ...
9
Heligmosomoides neopolygyrus Asakawa & Ohbayashi, 1986, a cryptic Asian nematode infecting the striped field mouse Apodemus agrarius in Central Europe
... rDNA, ITS1, 5.8S rDNA and ITS2) loci make it clear that this worm is quite distinct to ...the region of Novosibirsk [10], sug- gesting that this taxon is widespread in eastern Europe and western ...
10
Phylogeny and PCR identification of the human pathogenic fungus Penicillium marneffei
... Knowledge of the phylogenetic position of P. marneffei facilitated the design of unique oligonucleotide primers for use in the PCR to identify P. marneffei. Nucleotide sequence com- parisons of the ...
5
Multiplex PCR Using Internal Transcribed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains
... and ITS2 regions and the 5.8S ribosomal DNA (rDNA) region of the fungi were amplified by using universal primers ITS1 and ...The ITS2 region was simultaneously amplified by using ...
6
Genetic Characterization of Fasciola Isolates from West Azerbaijan Province Iran Based on ITS1 and ITS2 Sequence of Ribosomal DNA
... as ITS1, 5.8s, ITS2, COI, and ND1 (2, 8, 28, 42, ...of ITS1 region revealed that both ...of ITS1, 5.8s, and ITS2) construct- ed in the current study showed 100% similari- ty with ...
13
Rapid Identification of Yeasts in Positive Blood Cultures by a Multiplex PCR Method
... primers ITS1 and ITS2 to am- plify a conserved portion of the 18S rDNA region, the adjacent ITS1 region, and a small portion of the ...rDNA region, yielding products with ...
6
Genetic Diversity of Pneumocystis carinii f sp hominis Based on Variations in Nucleotide Sequences of Internal Transcribed Spacers of rRNA Genes
... The ITS2 sequences from the same speci- mens were also aligned and compared to the prototype se- ...13 ITS2 sequences reported previously ...the ITS1 sequences, sporadic ITS2 sequence ...
6
Phylogenetic analysis of Endophytes from Bitter Melon (Momordica charantia) in Guangdong Province
... The endophytic fungi associated with bitter melon comprise a number of cosmopolitan species such as Alter- naria alternata, Aspergillus fumigatus, Colletotrichum gloeosporioides and Curvularia lunata. All these genera ...
13
Genotypic Study of Pneumocystis jirovecii in Human Immunodeficiency Virus Positive Patients in Thailand
... To examine the genetic relationship of all 23 types of P. jirovecii found in Thailand, inferred trees under molecular phylogenetic methods were constructed from 498 aligned nu- cleotide positions of ...
7
Molecular phylogenetic history of eastern Mediterranean Alopiinae, a group of morphologically indeterminate land snails
... In order to evaluate the possibility of human-aided dispersal, divergence time can only be tentatively estimated, bearing in mind that these estimations rest on uncorroborated assumptions and may therefore be flawed. We ...
128