Knock-in mice
Measles Virus-Induced Immunosuppression in SLAM Knock-In Mice
... SLAM knock-in mice, in which the V domain of mouse SLAM was replaced by the V domain of human SLAM, crossed with alpha/beta-interferon receptor knockout mice, reproduced many immunological ...
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Live imaging and tracking of genome regions in CRISPR/dCas9 knock in mice
... and mice conditionally deleted for TRF1 [21, ...dCas9-EGFP knock-in mice could be used to study other mechanistic factors regulating the chromatin dynamics of telomeres and other genome regions in ...
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Genetic Contributors to Intergenerational CAG Repeat Instability in Huntington’s Disease Knock-In Mice
... CAG knock-in mouse models also exhibit a propensity for intergenerational repeat size ...HD knock-in mouse model breeding datasets reported to ...the knock-in ...between knock-in mice ...
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Ccdc6 knock-in mice develop thyroid hyperplasia associated to an enhanced CREB1 activity
... Ccdc6-ex2 knock-in ...wt mice to obtain germ line transmission with the generation of mice heterozygous for Ccdc6 gene ...heterozygous mice were then crossed each other to generate homozygous ...
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Genetic background modulates phenotypes of serotonin transporter Ala56 knock-in mice
... Biochemical and behavioral assays displayed a striking divergence of phenotypes between SERT Ala56 knock-in mice on the 129 versus on the B6 genetic background. Whereas a few tests showed quantitative ...
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Conditional activation of the N598R point mutation in the NR1 subunit of the NMDA receptor in knock-in mice
... The exact signal transduction mechanism by which the resulting rise in intracellular Ca^"^ triggers the enhancement of synaptic strength to express LTP remains to be established. Among the few molecules which are ...
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Tafenoquine and NPC 1161B require CYP 2D metabolism for anti malarial activity: implications for the 8 aminoquinoline class of anti malarial compounds
... 2D6 knock-in mice could largely be attributed to intrinsic differences between the two CYP 2D enzymes (human ...2D6 knock-in animals ver- sus those from the wild-type ...2D6 knock- in ...
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Recombinase mediated cassette exchange reveals the selective use of Gq/G11 dependent and independent endothelin 1/endothelin type A receptor signaling in pharyngeal arch development
... RMCE-mediated knock-in recombination was very high in our screening (44 to 87%), compared with conventional homologous ...with knock-in sequences containing mutant lox sites and an FRT-flanked ...Ednrb ...
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Hearing impairments caused by genetic and environmental factors
... in mice in which Y1062 in c-Ret was replaced with phenylalanine (c-Ret-KI Y1062F/Y1062F -mice), while hetero- zygous c-Ret Y1062F knock-in mice (c-Ret-KI Y1062F/? - mice) are reported ...
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Cloning free CRISPR/Cas system facilitates functional cassette knock in in mice
... of knock-in mice carrying gene cassettes in the endogenous ...any knock-in mice by the direct delivery of Cas9 protein, sgRNA, and targeting vector into the pronuclei of zygotes, the high ...
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Transgenic animal models for study of the pathogenesis of Huntington’s disease and therapy
... transgenic mice, which express N-terminal or full-length mutant huntingtin ubiquitously or selectively in different cell types, and knock-in mice that express full-length mutant Htt at the endogenous ...
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Targeting endothelial connexin40 inhibits tumor growth by reducing angiogenesis and improving vessel perfusion
... The mechanism(s) underlying the connexin effects remain(s) to be fully unraveled. Knocking down of Cx40 in HUVECs using specific siRNA, was already shown to decrease the formation of capillary-like structures [27]. We ...
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P-glycoprotein at the blood-brain barrier: kinetic modeling of 11C-desmethylloperamide in mice using a 18F-FDG μPET scan to determine the input function
... The determination of percent parent compound ( 11 C- dLop) in plasma over time was performed in WT (pre- treated with saline or 50 mg cyclosporine/kilogram body weight, n = 3 per group and per time point) and KO ...
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Development of autophagy inducers in clinical medicine
... in mice infected with West Nile virus or chikungunya ...in mice partially deficient in beclin 1, suggesting that the peptide acts through enhancement of ...
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Effect of hyperlipidemia on the expression of circadian genes in apolipoprotein E knock-out atherosclerotic mice
... postpartum) mice were purchased from Beijing Labora- tory Animal Research Center (Beijing, ...ApoE-/- mice were randomly divided into two groups: half of these mice (n = 18) were fed with regular ...
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Paeoniflorin attenuates incipient diabetic nephropathy in streptozotocin-induced mice by the suppression of the Toll-like receptor-2 signaling pathway
... diabetic mice. Our experiment showed that in the kidneys of WT + STZ mice, TLR2 and NF- κ B p65 signaling was acti- vated, along with a large number of infiltrating ...
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Original Article NLRP3 inflammasome is essential for the development of chronic obstructive pulmonary disease
... JAX Labs (Bar Harbor, ME) [18]. C57Bl/6 mice (Charles River, Boston, MA) were used as controls. For all the following experiments, 8 week old female mice, at least 6 in each group, were used. All animal ...
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β Catenin–regulated myeloid cell adhesion and migration determine wound healing
... lineage. Mice harboring a macrophage-specific deletion of the gene encoding β-catenin exhibited insufficient skin wound healing due to macrophage-specific defects in migration, adhesion to fibroblasts, and ability ...
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Absence of mitochondrial uncoupling protein 3: Effect on thymus and spleen in the fed and fasted mice
... UCP3 knock-out mice ...wild-type mice starvation results in an increase in the proportion the smaller subsets namely CD4SP, CD8SP and DN cells which in turn are offset by a 6% decrease in the ...
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Reduced synaptic function of Kainate receptors in the insular cortex of Fmr1 Knock-out mice
... KO mice, indicating that KARs may also participate in the pathology of the ...KO mice inhibit the expression of the cingulate ...KO mice alter catalytic and regulatory parts of PKA in the ACC ...
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