laser microdissection
Comparison in gene expression of secretory human endometrium using laser microdissection
... developed laser microdissection method has gained widespread use throughout the research ...using laser microdissection and differential display ...
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Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
... utilizing the HOPE-technique, which were then success- fully applied to in situ hybridization targeting mRNA or immunocytochemistry with excellent preservation of morphological details [10]. In this study we describe the ...
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A rapid preparation procedure for laser microdissection-mediated harvest of plant tissues for gene expression analysis
... were placed in a specimen holder and cut in 100 µm thick cross-sections using a vibratome (Fig. 1a, b). Taka- hashi et al. [20] reported that lowering the temperature at which sections are dehydrated from 42 to 4 °C, ...
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Original Article Laser microdissection-based analysis of cytokine balance in the kidneys of patients with ANCA-associated glomerulonephritis
... Abstract: Antineutrophil cytoplasmic autoantibody (ANCA)-associated systemic vasculitis (AASV) includes a group of disorders characterized by autoimmune inflammation affecting small- to medium-sized vessels, which leads ...
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Simple preparation of plant epidermal tissue for laser microdissection and downstream quantitative proteome and carbohydrate analysis
... complete, laser-microdissected epidermal defense structures, have not been ...by laser microdissection (LM) coupled to laser pressure ...
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A protocol for laser microdissection (LMD) followed by transcriptome analysis of plant reproductive tissue in phylogenetically distant angiosperms
... We optimized and accelerated a method based on fixa- tion and cryo-sectioning of plant tissue and combined this with the use of LMD film [11] to produce accurate sections of flowers of different species and developmen- ...
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Combination of laser microdissection, 2D-DIGE and MALDI-TOF MS to identify protein biomarkers to predict colorectal cancer spread
... patients. Laser micro-dissection was used to isolate cancer cells from primary colorectal tumors of stage II patients which did or did not metastasize within 5 years after surgical ...of laser ...
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High quality RNA isolation from Aedes aegypti midguts using laser microdissection microscopy
... After fixation with either Bouin’s or Carnoy’s fixative, 5-6 mosquito abdomens were dissected and embedded in the same orientation in O.C.T. Compound (Sakura Tissue- Tek, Torrence, CA) in aluminum foil cryomold (1.5 cm 3 ...
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PAT-H-MS coupled with laser microdissection to study histone post-translational modifications in selected cell populations from pathology samples
... In this study, we show the feasibility of applying tech- niques to reduce sample heterogeneity, including laser microdissection, to the MS-based analysis of hPTM achieved through the PAT-H-MS protocol that ...
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The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow
... UV laser microdissection (UV LMD) and IR laser capture microdissection (IR LCM) on phosphoprotein abundance of key cancer signaling protein targets assessed by reverse-phase protein microarray ...
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PAT-ChIP coupled with laser microdissection allows the study of chromatin in selected cell populations from paraffin-embedded patient samples
... Results: In this study, we tested the use of both core needle biopsies (CNBs) and laser microdissection (LMD), evaluating the compatibility of these methods with the PAT-ChIP procedure. Modifications of the ...
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Laser microdissection-based gene expression analysis in the aleurone layer and starchy endosperm of developing rice caryopses in the early storage phase
... phase. Laser microdissection (LM) is a powerful tool for isolating targeted individual cells from heterogeneous tissue viewed under a microscope, using an intense laser beam (Emmert-Buck et ...
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Optimised laser microdissection of the human ocular surface epithelial regions for microarray studies
... on laser microdissected tissue samples by Ohyama H et ...on laser microdis- sected breast tissue samples by Cowherd et ...in Laser Micro- dissection” (PALM®) microbeam systems (PALM Micro- ...
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Novel markers for differentiation of lobular and ductal invasive breast carcinomas by laser microdissection and microarray analysis
... Microdissection of normal and tumor cell types from the breast and full genome expression analysis by Affymetrix arrays allowed us to provide novel data on breast cancer. Invasive lobular and ductal breast ...
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Sorafenib Acts through VEGFR 2 Inhibition in a Metastatic Clear Cell Sarcoma of the Kidney
... we laser-microdissected CD34-expressing endothelial cells (Figure ...using laser-microdissection, we also included in this study laser-microdissected tumor endothelial cells from lung ...
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New insights into the protein aggregation pathology in myotilinopathy by combined proteomic and immunolocalization analyses
... Results: Skeletal muscle samples from 15 myotilinopathy patients were included in the study. Aggregate and control samples were collected from muscle sections by laser microdissection and subsequently ...
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Laser capture microdissection of gonads from juvenile zebrafish
... for microdissection are used instead of glass slides, the morphology is further ...allows microdissection of cryosections with impaired ...with laser microdissection and yielded RNA of a ...
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A novel procedure for the quantitative analysis of metabolites, storage products and transcripts of laser microdissected seed tissues of Brassica napus
... of laser micro-dissected (LM) tissues in oilseed rape (Brassica ...for laser microdissection of the seed, and the subsequent extraction and quantitative analysis of lipids, starch and metabolites ...
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Laser Capture Microdissection Assessment of Virus Compartmentalization in the Central Nervous Systems of Macaques Infected with Neurovirulent Simian Immunodeficiency Virus
... for laser capture studies, and the opposite hemisphere was used for ...were laser excised and pooled in a single tube, and a similar procedure was followed for the ...
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An improved procedure for isolation of high-quality RNA from nematode-infected Arabidopsis roots through laser capture microdissection
... Compatible interaction is best studied between cyst nematode Heterodera schachtii and the model plant Arabidopsis thaliana. In recent years a lot of work with this particular pathosystem has shown that the develop- ment ...
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