light sheet fluorescence microscopy (LSFM)
Three photon light sheet fluorescence microscopy
... in light-sheet fluorescence microscopy (LSFM) has revolutionized the field of imaging by using a thin sheet of light to optically section samples which are typically ...
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Light sheet fluorescence microscopy for neuroscience
... sub cellular resolution [47]. A more in depth discussion about optical clearing methods and their use in imaging mammalian brains with LSFM will be given in section 3.2. These methods are useful to image fixed samples, ...
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Image analysis in light sheet fluorescence microscopy images of transgenic zebrafish vascular development
... Chen et al. performed the only study to our knowledge addressing 3D ze- brafish neurovascular quantification using transgenic lines (visualized with con- focal microscopy) [27]. This quantified vessel length, ...
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PubMedCentral-PMC5752350.pdf
... Chagas disease is a parasitic infection caused by Trypanosoma cruzi, whose motility is not only important for localization, but also for cellular binding and invasion. Current animal models for the study of T. cruzi ...
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Integrating light-sheet imaging with virtual reality to recapitulate developmental cardiac mechanics
... Figure 2. Endocardial trabecular network in a transgenic Tg(cmlc2-gfp) zebrafish ventricle at 60 dpf. (A) VR accentuates the invaginating muscu- lar ridges in the apical region. (B–D) VR-LSFM enables navigation through ...
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Macro optical trapping for sample confinement in light sheet microscopy
... Light sheet fluorescence microscopy (LSFM) or selective plane illumination microscopy (SPIM) uses a thin sheet of light to illuminate a sample, whilst fluorescent images ...
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Multimode fibre : light sheet microscopy at the tip of a needle
... Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life ...the light-sheet through a multimode optical fibre – an optical ...
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Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications
... Light sheet microscopy: In contrast to the above-mentioned optical sectioning microscopy technologies, in light sheet microscopy, the illumination and detection paths are ...
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Greiss, Ferdinand (2017): Probing single molecules and binding networks: detection of single molecules on the multi-cellular scale with reflected light-sheet microscopy and probing the cooperativity of binding networks with high-throughput thermophoresis. Dissertation, LMU München: Fakultät für Physik
... In this study, we developed an automatized measurement platform to quantitatively investigate binding constants in reduced sample volumes of 500 nl and in high throughput with standardized 1,536-well plates. We first ...
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Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy
... of light and then detecting the fluorescence perpendic- ular to the direction of sheet propagation (139, 140) ...two-photon microscopy). Indeed, light-sheet mi- croscopy was ...
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Wavefront corrected light sheet microscopy in turbid media
... diffraction-limited performance deep within turbid tissue phantom samples at depths of up to six times the MFP. Opti- cal memory effects, resulting from the individual sample properties, were shown to allow isotropic ...
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Quantitative imaging of cell dynamics in mouse embryos using light sheet microscopy
... theta microscopy (Lindek et ...illumination microscopy (SPIM) (Huisken et ...plane fluorescence optical sectioning microscopy (Voie et ...a sheet of light to illuminate the ...
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Q&A: How can advances in tissue clearing and optogenetics contribute to our understanding of normal and diseased biology?
... Single-molecule RNA FISH is another labeling option in cleared tissue, first demonstrated in Yang et al. [10]. The ability to label RNAs is crucial to providing a ‘snap-shot’ of cell state at a precise time point. Recent ...
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Dynamic light sheet generation and fluorescence imaging behind turbid media
... the fluorescence signal could reach the camera directly without being ...the light sheet using its image on the ...of light sheet microscopes can be implemented as well ...scattered ...
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Selective plane illumination microscopy techniques in developmental biology
... the light-sheet and a loss in ...the light- sheet as it penetrates dense ...the light-sheet (and of the optical section) might increase with depth from ~5 μ m to 10-15 μ m in a ...
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Probing neural tissue with airy light sheet microscopy : investigation of imaging performance at depth within turbid media
... Adult female wild type mice were anaesthetised as described above. Fluorescent beads (Duke Scientific R600, 600nm diameter polystyrene, red fluorescence), diluted 1:50 in PBS, were stereotaxically injected with a ...
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Fluorescence Microscopy of Nicotinic Acetylcholine Receptors
... α5-mEGFP fluorescence is seen. In addition to reduced fluorescence, a cell death rate of over 50% is ...intensity fluorescence that is confined to the cell bodies (see figure ...detected ...
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Applications of Oxygen-Carrying Micro/Nanobubbles: a Potential Approach to Enhance Photodynamic Therapy and Photoacoustic Imaging
... Figure 3 shows an MNB containing oxygen with a photosensitizer drug incorporated in the shell. These MNBs can be used for PAI and PDT, and are more beneficial owing to the oxygen in the core. This yields the benefit of a ...
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4 Dimensional light sheet microscopy to elucidate shear stress modulation of cardiac trabeculation
... laser light-sheet approach known as selective plane illumination microscopy (SPIM) coupled with nongated 4D synchronization algorithm enabled us to capture live 3D cardiac ...of light and ...
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Anisotropic Diffusion in Fluorescence Microscopy
... FRAP (Fluorescence Recovery After Photobleaching) techniques can be used to measure the effective diffusion coefficients of tracer molecules within biological mate- rials like articular cartilage [1]. Because of ...
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